Expression of orexin B and its receptor 2 in rat testis

Liguori, Giovanna; Pavone, Luigi Michele; Assisi, Loredana; Langella, Emilia; Tafuri, Simona; Mirabella, Nicola; Costagliola, Anna; Vittoria, Alfredo

doi:10.1016/j.ygcen.2015.11.015

Cited by 23 publications

(32 citation statements)

References 41 publications

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“…OX 2 ‐IR was found both in the interstitial and the tubular compartments. These results also demonstrate the wide distribution of the two peptides in the male gonad in line with a previous study showing the localization of OxB and OX 2 in rat testis (Liguori et al., ). Our research also highlights a similarity between the orexinergic system of the alpaca testis and that described in the rat testis and suggests that this system could play a role in the regulation of spermatogenesis.…”

Section: Discussionsupporting

confidence: 93%

“…The consequent stimulation of testosterone production was experimentally assessed through the sequential in vitro addition of OxA and MIS, and vice versa, to slices of rat and alpaca testis (Assisi et al., ; Liguori et al., ). To test a potential steroidogenic effect of OxB in the male gonad, an experimental study was performed on in vitro cultured rat testis slices (Liguori et al., ) in which the steroidogenic activity of OxA was confirmed, but, conversely, OxB was found to be ineffective on testosterone production.…”

Section: Discussionmentioning

confidence: 99%

“…To test a potential steroidogenic effect of OxB in the male gonad, an experimental study was performed on in vitro cultured rat testis slices (Liguori et al, 2017) in which the steroidogenic activity of OxA was confirmed, but, conversely, OxB was found to be ineffective on testosterone production.…”

Section: Discussionmentioning

confidence: 99%

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Localization of orexin B and receptor 2 for orexins in testicular cytotypes of the camelid alpaca (Vicugna pacos)

Liguori

Squillacioti

Assisi

et al. 2017

Reprod Domestic Animals

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Contents The orexins A (OxA) and B (OxB) are two hypothalamic peptides involved in many physiological functions of the mammalian body. They act through the binding of two G‐coupled receptors named receptor 1 (OX1) and receptor 2 (OX2) for orexins. The first receptor is specific for OxA, while the second binds both the substances with equal affinity. The orexins and the relative receptors have been traced by means of different techniques also at the periphery of the body and particularly in the adrenals, and in gastrointestinal and genital organs. Aim of this work was to investigate the presence of OxB and OX2 by means of immunohistochemistry and Western blotting analysis in the testis of the South American camelid alpaca, a species primarily breed in Chile and Ecuador and recently diffused in Europe where the quality of its wool is particularly appreciated. OxB immunoreactivity (IR) was found in the tubular compartment of the testis where spermatogonia (resting), zygotene and pachytene spermatocytes, and spermatids clearly showed differently sized and shaped cytoplasmic positive structures. OX2‐IR was found both in the interstitial and tubular compartments of the testis and particularly in Leydig cells and round and elongated spermatids. Western blotting analysis of testis lysates showed the presence of a protein band whose molecular weight corresponded to that currently assigned to OX2. Such findings easily translate the hypothesis that OxB and its receptor 2 play a functional role both in the interstitial and tubular compartments of the alpaca testis.

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Section: Discussionsupporting

confidence: 93%

Section: Discussionmentioning

confidence: 99%

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Localization of orexin B and receptor 2 for orexins in testicular cytotypes of the camelid alpaca (Vicugna pacos)

Liguori

Squillacioti

Assisi

et al. 2017

Reprod Domestic Animals

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“…Primary antisera were the same as described in the previous section, 1:1,000 diluted. The specificity of the primary immunoreactions was tested by replacing each antibody with a buffer or preabsorbing the antibody with an excess (100 μg antigen/ml antiserum as the final dilution) of the relative antigen (Liguori et al, , ). No immunoreaction was detected in control tests.…”

Section: Methodsmentioning

confidence: 99%

Urocortinergic system in the epididymis of the normal and cryptorchid dogs

Squillacioti

Pelagalli

Luca

et al. 2019

Reprod Domestic Animals

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Cryptorchidism is associated with changes in the gonads and the spermatic duct system, which may cause infertility problems. Urocortin (UCN) is a corticotrophin‐releasing hormone (CRH)‐related peptide, which affects several functions of male genital organs. The aim of the present study was to investigate the expression of UCN and its receptors CRHR1 and CRHR2 using immunohistochemistry, western blotting and real‐time reverse transcription polymerase chain reaction in tissues collected from the epididymis of normal and cryptorchid dogs. The lumen of the cryptic epididymal duct was found to be relatively smaller than that of the normal one, and interstitial tissue was abundant in the cryptic epididymis. In addition, only a few spermatids were observed in the lumen of the epididymal duct. Results showed that UCN, CRHR2 and CRHR1 were expressed in tissues collected from normal and cryptic epididymal ducts. Urocortin‐ and CRHR2‐immunoreactivities (IRs) were detected in the principal cells of the caput, corpus and cauda of the normal and cryptic epididymides. CRHR1‐IR was detected in vascular smooth muscles and fibromuscular cells surrounding epididymal tubules of the normal and cryptorchid dogs. Expression levels of UCN and CRHR2 mRNA were higher in cryptic epididymal ducts than that in normal epididymal ducts. These results suggest that UCN and its receptors might play a role in regulating the maturation and storage of spermatozoa. These findings indicated that the expression of these proteins could be modulated by the cryptorchidism condition.

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“…Proteins were subjected to SDS-PAGE (12.5% polyacrylamide). After electrophoresis, proteins were transferred to nitrocellulose membranes (Immobilon, Millipore Corp., Bedford, MA) (Fiorito et al 2017b;Liguori et al 2017); complete transfer was assessed using pre-stained protein standards (Bio-Rad, Hercules, CA). After blocking with Tris-buffered saline-BSA [25 mM Tris (pH 7.4), 200 mM NaCl, and 5% BSA], the membrane was incubated with the primary antibodies.…”

Section: Protein Extraction and Western Blot Analysismentioning

confidence: 99%

2,3,7,8-Tetrachlorodibenzo-p-dioxin influences bovine herpesvirus 1 replication through upregulation of SIRT3 and cytoskeletal reorganization

et al. 2017

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Infection of kidney cells (MDBK) with Bovine Herpesvirus 1 (BoHV-1) is affected by 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD), which accelerates BoHV-1-induced apoptosis and increases virus replication. Herein, to elucidate the mechanism through TCDD modifies BoHV-1 infection, we analyzed the modulation of a members of Sirtuin proteins family in MDBK cells. We found that mitochondrial SIRT3 was upregulated during infection. This change was accompanied by cytoskeletal rearrangements and cell extensions. All these trends were drastically modified by TCDD. We hypothesize that, taken together, these results might further clarify the processes responsible for the action of TCDD on the BoHV-1 replication, resulting in enhanced virus production.

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Expression of orexin B and its receptor 2 in rat testis

Cited by 23 publications

References 41 publications

Localization of orexin B and receptor 2 for orexins in testicular cytotypes of the camelid alpaca (Vicugna pacos)

Localization of orexin B and receptor 2 for orexins in testicular cytotypes of the camelid alpaca (Vicugna pacos)

Urocortinergic system in the epididymis of the normal and cryptorchid dogs

2,3,7,8-Tetrachlorodibenzo-p-dioxin influences bovine herpesvirus 1 replication through upregulation of SIRT3 and cytoskeletal reorganization

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