Expression of osteogenic proteins during the intrasplenic transplantation of Meckel's chondrocytes: A histochemical and immunohistochemical study

Ishizeki, Kiyoto; Kagiya, Tadayoshi; Fujiwara, Naoki; Otsu, Keishi; Harada, Hidemitsu

doi:10.1679/aohc.72.1

Cited by 11 publications

(7 citation statements)

References 40 publications

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“…2 ). Following toluidine blue staining, red/purple particles in the cytoplasm were observed under a light microscope, which indicated the staining of proteoglycans ( 23 ) ( Fig. 2 ).…”

Section: Resultsmentioning

confidence: 99%

Bushen Zhuangjin decoction inhibits TM-induced chondrocyte apoptosis mediated by endoplasmic reticulum stress

Lin

Weng

Liu

et al. 2015

International Journal of Molecular Medicine

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Chondrocyte apoptosis triggered by endoplasmic reticulum (ER) stress plays a vital role in the pathogenesis of osteoarthritis (OA). Bushen Zhuangjin decoction (BZD) has been widely used in the treatment of OA. However, the cellular and molecular mechanisms responsible for the inhibitory effects of BZD on chondrocyte apoptosis remain to be elucidated. In the present study, we investigated the effects of BZD on ER stress-induced chondrocyte apoptosis using a chondrocyte in vitro model of OA. Chondrocytes obtained from the articular cartilage of the knee joints of Sprague Dawley (SD) rats were detected by immunohistochemical staining for type II collagen. The ER stress-mediated apoptosis of tunicamycin (TM)-stimulated chondrocytes was detected using 4-phenylbutyric acid (4-PBA). We found that 4-PBA inhibited TM-induced chondrocyte apoptosis, which confirmed the successful induction of chondrocyte apoptosis. BZD enhanced the viability of the TM-stimulated chondrocytes in a dose- and time-dependent manner, as shown by MTT assay. The apoptotic rate and the loss of mitochondrial membrane potential (ΔΨm) of the TM-stimulated chondrocytes treated with BZD was markedly decreased compared with those of chondrocytes not treated with BZD, as shown by 4′,6-diamidino-2-phenylindole (DAPI) staining, Annexin V-FITC binding assay and JC-1 assay. To further elucidate the mechanisms responsible for the inhibitory effects of BZD on TM-induced chondrocyte apoptosis mediated by ER stress, the mRNA and protein expression levels of binding immunoglobulin protein (Bip), X-box binding protein 1 (Xbp1), activating transcription factor 4 (Atf4), C/EBP-homologous protein (Chop), caspase-9, caspase-3, B-cell lymphoma 2 (Bcl-2) and Bcl-2-associated X protein (Bax) were measured by reverse transcription-polymerase chain reaction (RT-PCR) and western blot analysis. In the TM-stimulated chondrocytes treated with BZD, the mRNA and protein expression levels of Bip, Atf4, Chop, caspase-9, caspase-3 and Bax were significantly decreased, whereas the mRNA and protein expression levels of Xbp1 and Bcl-2 were significantly increased compared with the TM-stimulated chondrocytes not treated with BZD. Additionally, all our findings demonstrated that there was no significant difference between the TM-stimulated chondrocytes treated with BZD and those treated with 4-PBA. Taken together, our results indicate that BZD inhibits TM-induced chondrocyte apoptosis mediated by ER stress. Thus, BZD may be a potential therapeutic agent for use in the treatment of OA.

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“…2 ). Following toluidine blue staining, red/purple particles in the cytoplasm were observed under a light microscope, which indicated the staining of proteoglycans ( 23 ) ( Fig. 2 ).…”

Section: Resultsmentioning

confidence: 99%

Bushen Zhuangjin decoction inhibits TM-induced chondrocyte apoptosis mediated by endoplasmic reticulum stress

Lin

Weng

Liu

et al. 2015

International Journal of Molecular Medicine

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“…However, greater formation of new bone was observed in MZG (Fig. 3) suggesting that the C. ambrosioides cataplasm promotes osteogenesis and may serve as a stimulator of bone regeneration (Wong and Rabie, 2006;Pereira-Junior et al, 2007;Filho et al, 2009;Ishizeki et al, 2009).…”

Section: Discussionmentioning

confidence: 96%

Chenopodium ambroisioides in the Repair of Fractures in Rabbits

Neto¹,

Ribeiro²,

Morais³

et al. 2015

International J. of Pharmacology

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Chenopodium ambrosioides L. (Amaranthaceae), popularly known as "mastruz" or "erva-de-santa-maria", is a perennial plant found in Brazil and used in folk medicine for the treatment of contusions and fractures. The objective of this study was to evaluate the topical effect of a cataplasm prepared from fresh C. ambrosioides leaves on the treatment of fractures experimentally induced in rabbits. Thirty rabbits were divided into three groups (n = 10). After anesthesia, a radius fracture was created and the animals received topical applications of C. ambrosioides cataplasm (MZG), diclofenac sodium (DG) and isotonic solution 0.9% NaCl (Control). At 30 days, we evaluated the animal bone regeneration through both qualitative macroscopic analysis of the fracture focus as by determination of serum alkaline phosphatase (ALP). The evolution of bone repair has been verified at 30 and 45 days through the histological analysis. No significant difference in ALP levels was observed between groups. In addition, no allergic reactions or impairment of tissue adjacent to the fracture focus were seen in any of the groups. In contrast, although all groups exhibited similar tissue architecture, the histological analysis revealed greater formation of mature bone tissue in MZG at 30 days, when compared to DG and control group. In addition, higher collagen fiber density was observed in MZG at 45 days. These results indicate Chenopodium ambrosioides as a promising therapeutic agent for bone regeneration. The plant may be useful as a raw material for the production of biomaterials for fracture healing, contributing to its validation in ethnomedicine.

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“…Even though the intermediate part of Meckel’s cartilage initially serves as a template during the development of the lower jaw, it later degenerates, and a dentary bone emerges in its place, also known as the jawbone or the mandible. Although chondrocytes in Meckel’s cartilage have been shown to be able to transdifferentiate into osteogenic cells, evidence for the ossification of the intermediate part of Meckel’s cartilage in vivo is currently limited [ 27 , 28 , 29 , 30 , 31 , 32 ]. Most importantly, the cartilaginous matrix of Meckel’s cartilage is removed during the mandibular development.…”

Section: Anatomy and Fate Of Pharyngeal Archesmentioning

confidence: 99%

The Mandibular and Hyoid Arches—From Molecular Patterning to Shaping Bone and Cartilage

Fabik

Psutkova

Machoň

2021

IJMS

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The mandibular and hyoid arches collectively make up the facial skeleton, also known as the viscerocranium. Although all three germ layers come together to assemble the pharyngeal arches, the majority of tissue within viscerocranial skeletal components differentiates from the neural crest. Since nearly one third of all birth defects in humans affect the craniofacial region, it is important to understand how signalling pathways and transcription factors govern the embryogenesis and skeletogenesis of the viscerocranium. This review focuses on mouse and zebrafish models of craniofacial development. We highlight gene regulatory networks directing the patterning and osteochondrogenesis of the mandibular and hyoid arches that are actually conserved among all gnathostomes. The first part of this review describes the anatomy and development of mandibular and hyoid arches in both species. The second part analyses cell signalling and transcription factors that ensure the specificity of individual structures along the anatomical axes. The third part discusses the genes and molecules that control the formation of bone and cartilage within mandibular and hyoid arches and how dysregulation of molecular signalling influences the development of skeletal components of the viscerocranium. In conclusion, we notice that mandibular malformations in humans and mice often co-occur with hyoid malformations and pinpoint the similar molecular machinery controlling the development of mandibular and hyoid arches.

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Expression of osteogenic proteins during the intrasplenic transplantation of Meckel's chondrocytes: A histochemical and immunohistochemical study

Cited by 11 publications

References 40 publications

Bushen Zhuangjin decoction inhibits TM-induced chondrocyte apoptosis mediated by endoplasmic reticulum stress

Bushen Zhuangjin decoction inhibits TM-induced chondrocyte apoptosis mediated by endoplasmic reticulum stress

Chenopodium ambroisioides in the Repair of Fractures in Rabbits

The Mandibular and Hyoid Arches—From Molecular Patterning to Shaping Bone and Cartilage

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