Expression of P-glycoprotein is Positively Correlated with p53 in Human Papilloma Virus Induced Squamous Intraepithelial Lesions of Uterine Cervix: Poor Prognosis Association

Singh, Madhulika; Singh, Uma; Mathur, Neeraj; Shukla, Yogeshwer

doi:10.7314/apjcp.2012.13.12.6039

Cited by 4 publications

(1 citation statement)

References 28 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Multi drug resistance is considered to be a major cause of failure of conventional chemotherapy (Dong and Mumper, 2010). Some membrane proteins like P-glycoprotein 1 (Pgp1), multidrug resistance protein 1 (MRP1) belonging to ATP binding cassette (ABC) transporter family play important roles in the development of multidrug resistance (Kuo, 2009;Singh et al, 2012). Therefore overcoming drug resistance is a novel approach in cancer research.…”

Section: Introductionmentioning

confidence: 99%

Reversal of Resistance towards Cisplatin by Curcumin in Cervical Cancer Cells

Roy¹,

Mukherjee²

2014

Asian Pacific Journal of Cancer Prevention

View full text Add to dashboard Cite

Epigenetic regulators like histone deacetylases (1 and 2), and viral onco-proteins (E6/E7) are known to be overexpressed in cervical cancer cells. The present study was designed to investigate the effect of curcumin on HDACs (1 and 2) and HPV E6/E7 in the cervical cancer cell line SiHa and a drug resistant clone SiHa R (derived from SiHa). It was further intended to investigate whether curcumin could sensitize the cells towards cisplatin induced cell killing by modulation of multi drug resistant proteins like MRP1 and Pgp1. Curcumin inhibited HDACs, HPV expression and differentially increased acetylation and up-regulation of p53 in SiHa and SiHa R , leading to cell cycle arrest at G1-S phase. Up-regulation of pRb, p21, p27 and corresponding inhibition of cyclin D1 and CDK4 were observed. Cisplatin resistance in SiHa R due to over-expression of MRP1 and Pgp1 was overcome by curcumin. Curcumin also sensitized both the cervical cancer cells towards cisplatin induced cell killing. Inhibition of HDACs and HPVs led to cell cycle arrest at G1/S phase by alteration of cell cycle regulatory proteins. Suppression of MRP1 and Pgp1 by curcumin resulted in sensitization of cervical cancer cells, lowering the chemotherapeutic dose of the drug cisplatin.

show abstract

Section: Introductionmentioning

confidence: 99%