Expression of p16 in oral cancer and premalignant lesions

Buajeeb, Waranun; Poomsawat, Sopee; Punyasingh, Jirapa; Sanguansin, Sirima

doi:10.1111/j.1600-0714.2008.00710.x

Cited by 55 publications

(47 citation statements)

References 35 publications

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“…p16 Ink4a expression has been evaluated in several tumor types with very different results, ranging from its loss or downregulation (Brambilla et al, 1999a, b;Schneider-Stock et al, 2005;Ayhan et al, 2010) to its clear overexpression (Milde-Langosch et al, 2001;Armes et al, 2005;Zhao et al, 2006;Angiero et al, 2008;Buajeeb et al, 2009). The main objective of this review is to clarify these apparently contrasting expression patterns and to reveal their implications in cancer management.…”

Section: Introductionmentioning

confidence: 99%

p16Ink4a overexpression in cancer: a tumor suppressor gene associated with senescence and high-grade tumors

et al. 2011

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p16 Ink4a is a protein involved in regulation of the cell cycle. Currently, p16 Ink4a is considered a tumor suppressor protein because of its physiological role and downregulated expression in a large number of tumors. Intriguingly, overexpression of p16 Ink4a has also been described in several tumors. This review attempts to elucidate when and why p16 Ink4a overexpression occurs, and to suggest possible implications of p16 Ink4a in the diagnosis, prognosis and treatment of cancer.

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Section: Introductionmentioning

confidence: 99%

p16Ink4a overexpression in cancer: a tumor suppressor gene associated with senescence and high-grade tumors

et al. 2011

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“…Overexpression of p16 in HNSCC has also been shown [20,21]. This is still debated as other studies have proposed that there is a reduction in p16 expression [22,23]. …”

Section: Discussionmentioning

confidence: 86%

β-Catenin Expression in Oropharyngeal Squamous Cell Carcinomas: Comparison and Correlation with p16 and Human Papillomavirus in situ Hybridization

Nwanze

Cohen²,

Schmitt³

et al. 2015

Acta Cytologica

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Background: The Wnt/β-catenin signaling pathway has been noted to be upregulated in head and neck cancers, including oropharyngeal squamous cell carcinoma (OSCC). This study compared the efficacy of β-catenin immunohistochemistry (IHC), p16 IHC and automated human papillomavirus (HPV) in situ hybridization (ISH) in OSCC. Methods: Sixty-eight OSCCs (48 surgical specimens and 20 fine-needle aspirations) were evaluated. Nuclear staining only of β-catenin was assessed as 0-3+ intensity (relative to controls of benign squamous mucosa). p16 was interpreted as positive if 70% of tumor cells showed brown nuclear and cytoplasmic staining. HPV ISH was interpreted as positive if a minimum of one tumor cell showed brown punctate dot-like nuclear positivity. p16 IHC and HPV ISH were then correlated with β-catenin staining. HPV ISH was used as the gold standard. Results: Twenty-five of 48 surgical specimens (52.1%) and 11 of 20 cell blocks (55%) stained positively for β-catenin, making a total of 36 of 68 (52.9%) staining positively for β-catenin, as compared to 61.7% positive for p16 IHC and 70.6% positive by automated HPV ISH, the gold standard method for OSCC diagnosis. χ² analysis revealed no significant correlation between β-catenin and HPV ISH (p > 0.05) and demonstrated a strong correlation between p16 and HPV ISH (p < 0.05). Conclusion: β-Catenin IHC is not a sensitive or specific marker of HPV and is unlikely to be a useful adjunct to p16 IHC or HPV ISH in the setting of advanced OSCC. However, as this study focused on samples of advanced OSCC, β-catenin IHC may still find some use in the diagnosis of early-stage OSCC.

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“…Nuclear p16 expression has been described in adult but not fetal tissues in many organs (5) and in cells in culture reaching their limit of population doubling (15). Buajeeb et al (16) have reported that p16 is not reliable for oral mucosal dysplasia and malignant transformation, although it is possible that p16 plays a role in cell senescence in normal oral mucosa since the protein is detectable by Western blotting in normal oral mucosa (3). Sousa et al (17) have reported that no significant statistical difference of the cell proliferation rate between oral lichen planus and epithelial dysplasia (p>0.05) and between the epithelial dysplasia and oral squamous cell carcinoma (p>0.05) was observed.…”

Section: Discussionmentioning

confidence: 99%

An immunohistochemical study of the distribution of p 16 protein in oral mucosa in smokers, non-smokers and in frictional keratosis

Tarakji

Kujan²,

Nassani³

2010

Med Oral

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Objective: Our study aimed to characterize alteration in the immunohistochemical p16 expression in normal oral mucosa and non-neoplastic hyperproliferative disorders (i.e. frictional keratosis and mucosa from smokers). Study design: 43 specimen of oral mucosa were examined using immunohistochemistry. Results: In normal mucosa, there was strong positive nuclear staining in a proportion of fibroblasts and endothelial cells in the lamina propria, with variable expression in nuclei of the epithelial layer. However, when the patient's tobacco smoking was examined, p16 nuclear staining in oral epithelium was seen in 4/20 (20%) of smokers and 0/23 (0%) of non-smokers. In every case of frictional keratosis (n=11), there was strong nuclear staining of some basal and supra-basal cells. In addition, there was strong nuclear staining of parakeratinizing squamous in a band above the spinous layer. In clinically normal oral epithelium of smokers and in frictional keratosis, basal and supra-basal cells expressed strong p16 nuclear staining which was absent in the control tissue examined. Conclusion: Our data suggest that p16 expression may be involved in the long-term loss of proliferation in cell senescence of oral mucosa.

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Expression of p16 in oral cancer and premalignant lesions

Abstract: As a marker, p16 is not reliable for oral mucosal dysplasia and malignant transformation.

Cited by 55 publications

References 35 publications

p16Ink4a overexpression in cancer: a tumor suppressor gene associated with senescence and high-grade tumors

p16Ink4a overexpression in cancer: a tumor suppressor gene associated with senescence and high-grade tumors

β-Catenin Expression in Oropharyngeal Squamous Cell Carcinomas: Comparison and Correlation with p16 and Human Papillomavirus in situ Hybridization

An immunohistochemical study of the distribution of p 16 protein in oral mucosa in smokers, non-smokers and in frictional keratosis

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