Expression of P450-aromatase in the goat placenta throughout pregnancy

Mondragón, J.A.; Ocadiz-Delgado, Rodolfo; Miranda, Carolina Abreu; Valencia, Javier; Rosales, Ana M.; Gariglio, Patricio; Romano, Marta

doi:10.1016/j.theriogenology.2007.03.030

Cited by 11 publications

(5 citation statements)

References 29 publications

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“…Direct in situ RT‐PCR was performed as described by Nuovo (1996) with some modifications (Mondragón et al. 2007; Ocadiz‐Delgado et al.…”

Section: Methodsmentioning

confidence: 99%

“…In vitro expression of P450-Aro mRNA in the goat corpus luteum The tissues were processed following the procedure described previously in Mondrago´n et al (2007). The presence of aromatase and actin mRNAs was examined by RT-PCR.…”

Section: Methodsmentioning

confidence: 99%

“…In situ expression of P450-Aro mRNA in the sheep and goat corpus luteum Direct in situ RT-PCR was performed as described by Nuovo (1996) with some modifications (Mondrago´n et al 2007;Ocadiz-Delgado et al 2008, 2009). Briefly, dried dewaxed sections on silane-coated slides were incubated with protein lysis buffer (0.1 M Tris-HCl pH 8.0, 50 mM EDTA pH 8.0) containing 0.5 lg ⁄ ml Proteinase K for 30 min at room temperature.…”

Section: Methodsmentioning

confidence: 99%

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P450‐aromatase mRNA is Expressed in the Corpus Luteum (CL) of the Non‐Pregnant Sheep and Goat: The Expression of the Enzyme is Present Throughout Pregnancy in the Goat CL

Mondragón

Miranda

García‐Mena

et al. 2012

Reprod Domestic Animals

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In most mammals, the corpus luteum (CL) and placenta are the major sources of progesterone. The goat pregnancy depends on the presence of CL after mid-gestation, while sheep pregnancy does not. The expression and distribution of P450-aromatase (P450-Aro) mRNA throughout gestation has not been investigated in the goat CL and partially in the sheep CL. The present research was designed to characterize the expression of P450-Aro mRNA in small ruminant CL with emphasis in the goat. For this purpose, ovaries from Criollo goats and Pelibuey sheeps were analysed using in situ reverse transcription-polymerase chain reaction (RT-PCR) for the histological detection of P450-Aro transcripts. In addition, P450-Aro expression was determined by in vitro RT-PCR. In situ RT-PCR studies showed that the goat and sheep CL were rich in cells positive for P450-Aro mRNA. We have also found in vitro RT-PCR expression of P450-Aro mRNA in goat CL at 1, 3 and 4 months of gestation. This study shows that the goat CL expresses P450-Aro mRNA along gestation, suggesting that this structure is capable to produce oestrogens up to the end of gestation.

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“…Direct in situ RT‐PCR was performed as described by Nuovo (1996) with some modifications (Mondragón et al. 2007; Ocadiz‐Delgado et al.…”

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

P450‐aromatase mRNA is Expressed in the Corpus Luteum (CL) of the Non‐Pregnant Sheep and Goat: The Expression of the Enzyme is Present Throughout Pregnancy in the Goat CL

Mondragón

Miranda

García‐Mena

et al. 2012

Reprod Domestic Animals

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“…The activity of some drug metabolizing enzymes may be affected, either enhanced or decreased, by the action of the sexual steroids progesterone and oestradiol. During pregnancy, the expression of genes encoding hepatic CYP450 superfamily of microsomal enzymes may be decreased [16, 17, 32]. …”

Section: Pharmacokinetics In Pregnancymentioning

confidence: 99%

“…The placenta is able to metabolize xenobiotics since early pregnancy as this organ expresses various functionally active drug metabolizing enzymes [12, 14, 16, 17, 59–65]. Phase I metabolizing enzymes, such as CYP1A1, CYP4B1, and CYP19 (steroid aromatase, responsible of the placental conversion of androgens into estrogens) contribute to the oxidation of some xenobiotics in placenta [60, 61].…”

Section: Placental Functions and Drug Transfermentioning

confidence: 99%

Antibiotic Treatment of Dogs and Cats during Pregnancy

Rebuelto

Loza

2010

Veterinary Medicine International

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The use of pharmacological agents in pregnant females poses a major clinical challenge due to the marked physiological changes that may modify the pharmacokinetics of drugs and to the potential effects on the fetus. The purpose of this paper is to review briefly our knowledge on the use of antibacterial drugs during pregnancy and to provide information for the judicious selection of an antimicrobial treatment for use in pregnant bitches and queens. The risk to the fetus is a result of the ability of a drug to reach the fetal circulation and to produce toxic effects. The placenta functions as a barrier that protects the fetus due to the presence of transporters and metabolising enzymes; however, during pregnancy, the presence and activity of both enzymes and transporters may change. Antimicrobial agents that have been shown to be safe for use during pregnancy include betalactams, macrolides, and lincosamides. Pharmacotherapy during pregnancy in all species may affect adversely the developing fetus; therefore, it should be avoided when possible.

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Considerations in Pregnant or Lactating Patients

Kutzler¹

2013

Small Animal Toxicology

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Expression of P450-aromatase in the goat placenta throughout pregnancy

Cited by 11 publications

References 29 publications

P450‐aromatase mRNA is Expressed in the Corpus Luteum (CL) of the Non‐Pregnant Sheep and Goat: The Expression of the Enzyme is Present Throughout Pregnancy in the Goat CL

P450‐aromatase mRNA is Expressed in the Corpus Luteum (CL) of the Non‐Pregnant Sheep and Goat: The Expression of the Enzyme is Present Throughout Pregnancy in the Goat CL

Antibiotic Treatment of Dogs and Cats during Pregnancy

Considerations in Pregnant or Lactating Patients

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