Expression of p53 and its mechanism in prostate cancer

Wan, Jiukai; Zhang, Jun; Zhang, Junqiang

doi:10.3892/ol.2018.8680

Cited by 8 publications

(5 citation statements)

References 16 publications

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“…VIM3 and TP53 expression was highest in PC3 cells. TP53 is known to be overexpressed in prostate cancer and knockdown of TP53 results in a slower migration of prostate cancer cells (31). Our research group also showed that VIM3 protein levels increased significantly with increasing biological aggressiveness of human prostate cancer specimens from prostate biopsies/radical prostatectomy specimens (10).…”

Section: Discussionsupporting

confidence: 57%

Improved Analysis of Prostate Cancer: VIM3, ATG7 and P53 Form a Complex and Activate miRNA 371a-3p

NOHL,

BEHRING,

KAMERI

et al. 2023

Anticancer Res

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Background/Aim: It is not possible to differentiate prostate carcinomas sufficiently to ensure that every patient receives the right therapy. New molecular markers are needed. Our objective was to identify a complex consisting of vimentin variant 3 (VIM3), autophagy-related protein 7 (ATG7) and tumor protein p53 (TP53) in prostate cancer cells and its effect on microRNA (miR)-371a-3p. Materials and Methods: Prostate cancer cell lines (PC3, DU145, LNCaP) and the benign prostatic hyperplasia cell line BPH-1 were cultured in growth medium for 24 h, then stimulated with endothelin 1 (EDN1) (50 nM) and withaferin A (2 nM) for 24 h. Cell extracts were then analyzed by western blot. The localization of VIM3, ATG7 and TP53 in the nucleus was demonstrated with immunofluorescence staining and complex formation was demonstrated by immunoprecipitation. Cancer cell migration was analyzed with a scratch assay and agarose drop analysis. The binding of the complex to the promoter of pri-miR-371a-3p was analyzed with a non-radioactive electrophoretic mobility shift assay. VIM3 knockdown using small interfering RNA and quantitative real-time polymerase chain reaction for miR-371a-3p were performed. Results: The complex was present in the nucleus of prostate cancer cells and in the BPH-1 cell line. EDN1 increased the levels of the complex partners and cell migration, whereas withaferin A reduced the levels of the complex partners and migration. The complex bound to the promoter of pri-miR-371a-3p and might be involved in its transcription. Transfection with miR-371a-3p increased migration of prostate cancer cells. VIM3 knockdown reduced miR-371a-3p expression. Conclusion: The VIM3-ATG7-P53 complex, with its stimulatory effect on miR-371a-3p, may have the potential to be a marker for improved differentiation between prostate carcinomas, allowing tailored therapy. Recently, vimentin variant 3 (VIM3; PubMed: ACA06103.1) was discovered as a potential surrogate marker for prostate cancer (10). VIM3 is related to vimentin, an intermediate filament in mesenchymal and muscle cells, but with the important difference that it lacks the C-terminal ending of full-2407

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Section: Discussionsupporting

confidence: 57%

Improved Analysis of Prostate Cancer: VIM3, ATG7 and P53 Form a Complex and Activate miRNA 371a-3p

NOHL,

BEHRING,

KAMERI

et al. 2023

Anticancer Res

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“…While there is no speci c study focusing on NLRP1 protein expression in lung adenocarcinoma and prostate adenocarcinoma compared to normal tissues, Liang et al reported a substantial increase in NLRP1 protein level in patients with prostate cancer compared to those with prostate hyperplasia. Notably, the protein expression levels of NLRP1 showed a positive correlation with the Gleason score of prostate cancer [43]. Although the NLRP1 RNA expression was downregulated in LUAD and PRAD, the decrease in protein levels in LUAD was anticipated, while the increase in protein levels in PRAD was unexpected.…”

Section: Discussionmentioning

confidence: 91%

“…Furthermore, the downregulation of NLRP1 is correlated with unfavorable DFS in CHOL. Several studies have explored the potential of NLRP1 as a prognostic biomarker in various cancers, including lung adenocarcinoma [39,40,44], gastric cancer [45], prostate cancer [43], pancreatic cancer [46,47], head and neck squamous cell carcinoma [48], and laryngeal carcinoma [49]. Shen et al con rmed that decreased expression of NLRP1 was signi cantly associated with a poor prognosis of LUAD [41].…”

Section: Discussionmentioning

confidence: 99%

The NLRP1 inflammasome emerges as a promising therapeutic target and prognostic biomarker across multiple cancer types: A comprehensive pan-cancer analysis

Habibipour,

Sadeghi,

Raghibi

et al. 2024

Preprint

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Aims: Nod-like receptor family pyrin domain containing 1 (NLRP1) serves as the central component of the inflammasome complex and has emerged as a potential contributor to cancer development. Despite accumulating evidence, a comprehensive assessment of NLRP1 across various cancer types has yet to be undertaken. Main Methods: Several databases have evaluated NLRP1 expression across various cancer types in The Cancer Genome Atlas (TCGA). Additionally, studies have investigated the correlation between NLRP1 and various survival metrics, infiltration of cancer-associated fibroblasts, genetic alterations, drug sensitivity, and promoter methylation. Furthermore, research has explored the potential roles of NLRP1 and its interactions with other proteins. Key Findings: Our analysis revealed decreased expression of NLRP1 in BLCA, BRCA, KICH, LUAD, LUSC, PRAD, and UCEC tumor tissues compared to normal tissues. We identified a significant correlation between NLRP1 expression and various cancer survival parameters, genetic mutations, and immune infiltration of cancer-associated fibroblasts. Furthermore, we observed that NLRP1 expression is regulated by promoter DNA methylation in ESCA. Abnormal expression of NLRP1 was associated with decreased sensitivity to multiple anti-tumor drugs and small compounds. NLRP1 was found to be involved in pathways associated with T cell receptors and chemokines. Significance: Reduced NLRP1 expression contributes to cancer progression and holds potential as a crucial biomolecular marker for diagnostic, prognostic, and personalized therapeutic interventions across different malignancies.

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“…Mutations in p53 cause the cell cycle to be dysregulated, which leads to aberrant proliferation and neoplastic transformation since p53 is a repressive regulator of cell growth 48 . Researches have shown that aberrant p53 expression is directly related to tumor lymphovascular invasion, clinical stage, and clinicopathology in malignant cells 49,50 . But it is still unknown how aberrant p53 expression influences PC cells' malignant growth, metastasis, and differentiation.…”

Section: Resultsmentioning

confidence: 99%

Comparative Study of Genomic DNA Extraction Protocols from Whole Blood for P53 Gene Polymorphism in Persons with and without Prostate Cancer

Nasif

2024

Baghdad Sci.J

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In latest decades, genetic methods have developed into a potent tool in a number of life-attaching applications. In research looking at demographic genetic diversity, QTL detection, marker-assisted selection, and food traceability, DNA-based technologies like PCR are being employed more and more. These approaches call for extraction procedures that provide efficient nucleic acid extraction and the elimination of PCR inhibitors. The first and most important stage in molecular biology is the extraction of DNA from cells. For a molecular scientist, the high quality and integrity of the isolated DNA as well as the extraction method's ease of use and affordability are crucial factors. The present study was designed to establish a simple, fast and inexpensive method for DNA extraction from human peripheral blood (normal male n=2, age 24 years old, patient male (prostate cancer) n=2, age 65 years old) by comparing between them, and aimed to standardize a protocol of DNA extraction using five extraction protocols. The first method was the modified organic method by using sodium perchlorate instead of organic solvent (phenol, chloroform), sodium perchlorate advantage comes from its cheap price and low storage and shipping requirements, the second was the enzymatic method by using proteinase K, third method was done by using detergent, the fourth used phenol-chloroform; finally fifth one was salting out method. The result showed that the organic method gives a good DNA yield and needs relatively short time while the enzymatic method gives an excellent DNA purity which are more suitable for PCR by comparing five protocols using the spectrophotometer and Nanodrop technetium in addition to electrophoresis. Through the use of the five suggested procedures, the PCR multiplication of the P53 gene with the isolated DNA was effectively carried out. This indicates that, with the exception of the detergent approach, there were no significant inhibiting substances for Taq polymerase in the final solution.

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Expression of p53 and its mechanism in prostate cancer

Cited by 8 publications

References 16 publications

Improved Analysis of Prostate Cancer: VIM3, ATG7 and P53 Form a Complex and Activate miRNA 371a-3p

Improved Analysis of Prostate Cancer: VIM3, ATG7 and P53 Form a Complex and Activate miRNA 371a-3p

The NLRP1 inflammasome emerges as a promising therapeutic target and prognostic biomarker across multiple cancer types: A comprehensive pan-cancer analysis

Comparative Study of Genomic DNA Extraction Protocols from Whole Blood for P53 Gene Polymorphism in Persons with and without Prostate Cancer

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