Expression of paraoxonase types 1, 2 and 3 in reproductive tissues and activity of paraoxonase type 1 in the serum and seminal plasma of bulls

Ferreira, C. E. R.; Haas, Cristina; Goularte, Karina Lemos; Rovani, Monique Tomazele; Cardoso, F. F.; Schneider, Augusto; Gasperin, Bernardo Garziera; Lucia, Thomaz

doi:10.1111/and.12923

Cited by 6 publications

(7 citation statements)

References 34 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Our findings indicate that, among all tested sources, the PON1 activity in the sperm-rich portion of ejaculates may be a candidate biomarker for boar fertility, as indicated elsewhere (Barranco et al, 2015b;Ferreira et al, 2018), since ejaculates with increased PON1 activity in the sperm-rich portion would be more likely to have increased sperm concentration and greater frequency of spermatozoa with DNA integrity. In the present study, the two boars presenting the greatest PON1 activity in the sperm-rich portion of their ejaculates (Boars 2 and 3) were also those presenting the greatest sperm concentration, reflecting the fact that the sperm-rich portion presents greater sperm concentration (Peña et al, 2004).…”

Section: Discussionsupporting

confidence: 56%

“…Reduced PON1 activity was observed in the seminal plasma of men presenting teratozoospermia, azoospermia, subfertility and infertility (Verit et al, 2009;Gulum et al, 2017;Ozer et al, 2019), whereas increased PON1 activity was positively associated with sperm concentration (Ozer et al, 2019). In bulls, PON1 activity in the seminal plasma presented positive correlations with a subjective indicator of sperm concentration and with several parameters of sperm quality (Ferreira et al, 2018). In boars, greater PON1 activity in the seminal plasma was positively correlated with sperm concentration (Barranco et al, 2015b) and associated with increased motility, reduced production of ROS (Barranco et al, 2015a), improved cryotolerance (Li et al, 2018) and increased farrowing rates after AI (Barranco et al, 2015b).…”

Section: Introductionmentioning

confidence: 91%

“…In the serum, PON1 is associated with highdensity lipoprotein (HDL-C) (Mackness et al, 1998). The PON1 has been identified in the seminal plasma of men (Verit et al, 2009), roosters (Khan et al, 2012), bulls (Ferreira et al, 2018) and boars (Barranco et al, 2015a). In boars, the PON1 is expressed in the epididymis epithelium and in the secretory epithelium of the accessory sex glands (Barranco et al, 2017).…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Paraoxonase 1 activity in the sperm-rich portion of boar ejaculates is positively associated with sperm quality

et al. 2022

Self Cite

View full text Add to dashboard Cite

Associations of the activity of the paraoxonase 1 (PON1) enzyme with boar sperm quality still needs to be characterized, since boar ejaculates present distinct portions with differences in sperm concentration and quality. This study evaluated PON1 activity in the serum, in the distinct portions of boar ejaculates and estimated correlations with sperm quality parameters. Ejaculates and blood samples were collected from six boars for three weeks (two per week per boar; n = 36). Serum and post-spermatic portion PON1 activities were positively correlated (P = 0.01) but were both uncorrelated with the PON1 activity in the sperm-rich portion and in the whole ejaculate (P > 0.05). Differences in PON1 activity among boars were only observed in the sperm-rich portion of the ejaculate (P < 0.05). The PON1 activity in the serum and in the post-spermatic portion was generally negatively correlated with parameters of spermatozoa kinetics (P < 0.05). In the sperm-rich portion, PON1 activity was positively correlated with sperm concentration (P < 0.0001), curvilinear distance and velocity (both P < 0.05) and DNA integrity (P < 0.05), but negatively correlated with straightness and linearity (P < 0.05). Thus, boar ejaculates with increased PON1 activity in the sperm-rich portion may present increased concentration and spermatozoa with acceptable curvilinear velocity and distance and DNA integrity, which suggests that PON1 activity may be a biomarker for potential fertility.

show abstract

Section: Discussionsupporting

confidence: 56%

Section: Introductionmentioning

confidence: 91%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Paraoxonase 1 activity in the sperm-rich portion of boar ejaculates is positively associated with sperm quality

et al. 2022

Self Cite

View full text Add to dashboard Cite

show abstract

“…Indeed, several imprinted genes were related to behavioral functions. The second group, which involved the imprinted gene Ube3a , has been reported to regulate sleep homeostasis 57 ; the fifth group, which involved the imprinted gene Ntm , has been reported to be involved in motor function 58 ; the thirteenth group involved four imprinted genes ( Gpr1 , Nnat , Htra3 , and Pon2) , which regulate reproduction 59–62 ; and the fourteenth group involved four imprinted genes ( Dcd , 63 Ppo1r9a , 64 Slc22a2 , 65 and Pde4d 66 ) reported to mediate learning and memory.…”

Section: Discussionmentioning

confidence: 99%

Mouse hybrid genome mediates diverse brain phenotypes with the specificity of reciprocal crosses

et al. 2022

View full text Add to dashboard Cite

Genome hybridization serves as an evolutionary force for diversification, species modification, and adaption. 1 Genome hybridization contributes to novel patterns of gene expression through the interaction of two divergent genomes or transcriptional networks in plants and drosophila. 2 Genome hybridization in cichlid fish showed that phenotypic novelty was associated with increased genetic distance between species. 3 Gene expression in offspring is affected by parental origin through transgenerational epigenetic modification. Complex traits such as

show abstract

“…BSP5 presented a positive correlation with abnormal sperm in fresh semen, but negative with abnormal sperm in frozen-thawed semen (Menezes et al 2017). Spermadhesin 1 and paraoxonase (PON1) were negatively correlated with sperm vigor, whereas CLU exhibited a positive association with this parameter (Menezes et al 2017;Ferreira et al 2017). BSP5 and CLU had positive correlations with the viability of bovine sperm (Menezes et al 2017), and seminal gamma glutamyl transferase (GGT) activity showed positive associations with in vitro embryo development (Pero et al 2017).…”

Section: Tablementioning

confidence: 99%

Proteomic applications for studies of bull fertility

Viana¹

View full text Add to dashboard Cite

During spermatogenesis, the immature spermatozoon undergoes a series of morphological changes, and the nucleus is highly condensed to protect the DNA. Thus, the apparently transcriptionally silent sperm exclusively depends on the extracellular factors for survival and functionality. The seminal plasma (SP) is a fluid produced by accessory sexual glands, epididymides, and testes, and plays crucial roles in the ejaculated sperm. Understanding the complexity of the SP proteins is still a challenge and proteomic approaches have been widely used to uncover SP proteins and, consequently, potential protein biomarkers of bull fertility. To verify the state of the art of the SP proteome and its relationship with sperm fertility in bulls, we applied an extensive and systematic literature search to select 71 and 29 studies for systematic review and meta-analysis research, respectively. Qualitatively, the exposure to a single protein, such as osteopontin (OPN), binder of sperm proteins (BSPs), and heparin- binding proteins (HBPs), positively associates with sperm motility, capacitation, and fertilizing ability. Quantitatively, our meta-analysis revealed that seminal proteins ameliorate sperm parameters. BSPs and beta-defensin 126 highly favored sperm protection when cells were collected from the epididymis by retrograde flux and analyzed under room temperature conditions. Total SP proteins improved the motility and quality of Bos taurus sperm collected by artificial vagina, mainly in the presence of HBPs. Due to the diversity of methodologies applied in the eligible studies, the translational aspects of SP research should be taken into consideration to precisely define how seminal proteins can be harnessed to advance reproductive biotechnology. Then, we suggested that future studies should evaluate intact proteins to ascertain their biological functions. To address this question, we evaluated the associations between seminal proteoforms in bulls with contrasting freezability statuses. We employed top-down mass spectrometry to generate sperm and seminal plasma proteoform atlas of bulls with low (LF; n = 6 ) and high (HF; n = 9) semen freezability. Sperm and seminal plasma proteins were fractionated by tandem size exclusion chromatography (< 30 kDa) and analyzed by mass spectrometry (operated in protein intact mode). We characterized 299 and 267 bull SP and sperm proteoforms, respectively; most of them, truncated. Interestingly, the two proteins that presented the greatest number of proteoforms play opposite roles in the regulation of calcium intake, the c-type natriuretic peptide and the caltrin. A truncated proteoform of beta-defensin 10 with pyrrolidone carboxylic acid in the N-terminal was exclusively identified in the SP of HF bulls and may be further investigated to be pointed out as a potential biomarker. Eight truncations of OPN, including one with a serine phosphorylation site, were uniquely expressed in the SP of LF bulls. Regarding sperm proteoforms, we described the acetylation of histone H2A type 1 and c-Myc protein binding proteoform in LF sires, which may indicate a deficiency in DNA packing and/or nuclear activity. This first sperm and seminal plasma proteoform atlas of any species, showed that post-translational processing appears to define bio-attributes of proteins and their empirical associations with sperm cryoresistance. Keywords: Bull. Fertility. Seminal plasma. Proteins. Proteoforms. Systematic review. Meta- analysis.

show abstract

Expression of paraoxonase types 1, 2 and 3 in reproductive tissues and activity of paraoxonase type 1 in the serum and seminal plasma of bulls

Cited by 6 publications

References 34 publications

Paraoxonase 1 activity in the sperm-rich portion of boar ejaculates is positively associated with sperm quality

Paraoxonase 1 activity in the sperm-rich portion of boar ejaculates is positively associated with sperm quality

Mouse hybrid genome mediates diverse brain phenotypes with the specificity of reciprocal crosses

Proteomic applications for studies of bull fertility

Contact Info

Product

Resources

About