Expression of Parathyroid Hormone-Related Protein (PTHrP) mRNA in Mammary Gland of Periparturient Cows

Onda, Ken; Matsuki, Naoaki; Ono, Kenichiro; Wada, Yasunori

doi:10.1292/jvms.68.9

Cited by 5 publications

(6 citation statements)

References 16 publications

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“…In milk goat, PTHrP was found to be highly expressed in the mammary gland (Yang et al, 2010). Past studies in cow showed that PTHrP was expressed in the mammary gland during late pregnancy and increased subsequently with lactation, suggesting that PTHrP is involved in regulating local Ca homeostasis (Wojcik et al, 1998;Onda et al, 2006b). The physiological function of a protein may indicate its tissue distribution.…”

Section: Discussionmentioning

confidence: 99%

Identification, molecular characterization, and tissue expression of parathyroid hormone-related protein gene (PTHrP) from water buffalo (Bubalus bubalis)

Liu¹,

Qian²,

Huo³

et al. 2015

Genet. Mol. Res.

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ABSTRACT. Parathyroid hormone-related protein (PTHrP) is involved in the deposition of milk calcium in mammal lactation, but its role in buffalo is unclear. In this study, the full-length coding sequence of the water buffalo PTHrP gene was first isolated using reverse transcriptionpolymerase chain reaction. The protein was then subjected to molecular characterization using bioinformatic methods, and the tissue expression pattern was further assayed by semi-quantitative reverse-transcription polymerase chain reaction. The water buffalo PTHrP gene contains an open reading frame of 534 base pairs encoding a polypeptide of 177 amino acid residues, a theoretical molecular weight of 20.32 kDa, and an isoelectric point of 10.00. In addition, water buffalo PTHrP was predicted to contain a signal peptide, a typical hydrophobic region with no hydrophobic transmembrane regions, and to exert its function in the cell nucleus. A conserved domain of parathyroid superfamily from amino acids 34-114 was observed in the polypeptide. Sequence comparison and the phylogenetic analysis showed that the sequence of the water buffalo PTHrP protein shared high homology with that of other mammals, particularly cattle and goat. Among the 16 tissues examined, the PTHrP gene was only expressed in adipose tissue, placenta, uterine wall, hypophysis, and mammary gland tissue, but gene expression levels were higher in the uterus wall and adipose tissue. The results of this study suggest that the PTHrP gene plays an important role in the deposition of milk calcium of water buffalo.

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Section: Discussionmentioning

confidence: 99%

Identification, molecular characterization, and tissue expression of parathyroid hormone-related protein gene (PTHrP) from water buffalo (Bubalus bubalis)

Liu¹,

Qian²,

Huo³

et al. 2015

Genet. Mol. Res.

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“…Later studies have shown that PTHrP is produced not only in neoplastic tissues but also by many healthy human and animal tissues: thyroid gland, parathyroid gland, pituitary gland, salivary gland, urinary bladder, kidney, mammary gland, testis, epididymis, cartilage, osteoblasts, vascular smooth muscle cells [2,[8][9][10][11][12][13][14][15][16][17][18][19][20][21][22][23][24][25][26]. PTHrP is a very conservative peptide.…”

Section: Introductionmentioning

confidence: 99%

Immunolocalization of PTHrP in the parotid glands of three rodents species: Clethrionomys glareoulus, Microtus arvalis and white Swiss mice.

Czykier

Zabel²,

Seidel³

2010

Folia Histochem Cytobiol.

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Abstract:The current study was inspired by the fact that since 2004 no report had appeared on the occurrence of this peptide in healthy parotid glands of humans and animals. The objective of the current study was to investigate the immunolocalization of PTHrP in the parotid gland of three male rodents: 6 common voles (Microtus arvalis, Pallas, 1779), 6 bank voles (Clethrionomys glareoulus, Schreber, 1780) and 6 white Swiss mice, as well as to find out any species differences in the distribution of this peptide in various types of cells of the parotid gland. Immunocytochemical reactions were performed using the ABC technique with specific rabbit antibodies against human PTHrP (34-53) (CALBIOCHEM), diluted 1:70 and 1:50. We observed positive PTHrP expression in the epithelial cells of the striated duct in all the three animal species. The expression was strong in white mouse and very strong in common vole and bank vole. In all the rodent species studied, the reaction for PTHrP was granular in nature and irregularly distributed in the cytoplasm, being definitely stronger at the base and weaker at the apex of the cells. The PTHrP expression was negative in the epithelium of the intercalated duct, interlobular duct, main excretory duct, as well as in the myoepithelial cells surrounding the excretory ducts or serous acini.

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“…The expression of parathyroid hormone-related protein (PTHrP) mRNA in the mammary gland was reported to increase dramatically at the onset of the lactation in rats and cows [12,20]. On the immunohistochemical findings, PTHrP was detected in the lactating mammary gland, especially in epithelial cells and in the lumen, suggesting that mammary PTHrP regulates Ca uptake and/or release in the mammary gland [12,13].…”

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confidence: 99%

Parathyroid Hormone-Related Protein (PTHrP) and Ca Levels in the Milk of Lactating Cows

Onda

Sato

Yamaguchi

et al. 2006

The Journal of Veterinary Medical Science

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ABSTRACT. To investigate the roles of mammary PTHrP in calcium uptake and/or release in the mammary gland of cows, plasma PTHrP and Ca levels, and their arterial-venous differences were examined in a Jersey cow during the periparturient period. Levels of Ca in both abdominal aorta and abdominal subcutaneous vein blood slightly decreased around the parturition and at 24 days after the parturition, however, no remarkable arterial-venous differences were observed. Plasma PTHrP levels in both arterial and venous samples were below the detection limit (0.57 pmol/l) during the experimental period. Milk PTHrP and Ca levels were measured in 9 Holstein dairy cows. Although plasma PTHrP levels in all arterial and venous samples were also below the detection limit, milk PTHrP and Ca levels were remarkably high, ranging from 14,900 pmol/l to 41,200 pmol/l and from 772 mg/l to 1,200 mg/l, respectively. In addition, a significant positive correlation (P<0.01) was observed between milk PTHrP and Ca levels. These results suggested that mammary PTHrP is closely related to Ca concentration in the milk.

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Expression of Parathyroid Hormone-Related Protein (PTHrP) mRNA in Mammary Gland of Periparturient Cows

Cited by 5 publications

References 16 publications

Identification, molecular characterization, and tissue expression of parathyroid hormone-related protein gene (PTHrP) from water buffalo (Bubalus bubalis)

Identification, molecular characterization, and tissue expression of parathyroid hormone-related protein gene (PTHrP) from water buffalo (Bubalus bubalis)

Immunolocalization of PTHrP in the parotid glands of three rodents species: Clethrionomys glareoulus, Microtus arvalis and white Swiss mice.

Parathyroid Hormone-Related Protein (PTHrP) and Ca Levels in the Milk of Lactating Cows

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