Expression of Perilipin 2 (<scp>PLIN</scp>2) in Porcine Oocytes During Maturation

Zhang, R N; Fu, Xiangwei; Jia, Baoyu; Liu, C; Cheng, Keren; Zhu, Shi-En

doi:10.1111/rda.12386

Cited by 19 publications

(13 citation statements)

References 42 publications

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“…This suggests that higher FA concentrations, including saturated FAs in prepubertal FF, may have a regulatory effect on the expression of genes related solely to lipid metabolism during embryo development. Our results showed similar levels of PLIN2 expression in oocytes of P and C pigs and no change following in vitro maturation, consistent with other results in porcine and bovine oocytes 53 . Perilipins were found in various tissues, while only PLIN2 was present in oocytes of different species showing lipid droplet coating distribution.…”

Section: Discussionsupporting

confidence: 92%

The consequences of porcine IVM medium supplementation with follicular fluid become reflected in embryo quality, yield and gene expression patterns

Pawlak

Warzych

Cieślak

et al. 2018

Sci Rep

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Oocyte and embryo developmental competence are shaped by multiple extrinsic and intrinsic factors. One of the most extensive research areas in the last decade is the regulation of lipid metabolism in oocytes and embryos of different species. We hypothesized that differences in developmental competence of oocytes and embryos between prepubertal and cyclic gilts may arise due to distinct fatty acid profiles in follicular fluid. We found that supplementation of oocyte maturation media with follicular fluid from prepubertal pigs affected quality and development of embryos from prepubertal pigs while embryos of cyclic pigs were not affected. PLIN2, SCD and ACACA transcripts involved in lipid metabolism were upregulated in embryos originating from oocytes of prepubertal pigs matured with autologous follicular fluid. The surface occupied by lipid droplets tend to increase in oocytes matured with follicular fluid from prepubertal pigs regardless oocyte origin. The change into follicular fluid of cyclic pigs increased the efficiency of embryo culture and improved quality, while gene expression was similar to embryos obtained from cyclic gilts. We assume that the follicular fluids of prepubertal and cyclic pigs influenced the quality of oocytes and embryos obtained from prepubertal pigs which are more susceptible to suboptimal in vitro culture conditions.

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Section: Discussionsupporting

confidence: 92%

The consequences of porcine IVM medium supplementation with follicular fluid become reflected in embryo quality, yield and gene expression patterns

Pawlak

Warzych

Cieślak

et al. 2018

Sci Rep

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“…PLIN1, PLIN2 and PLIN3 are members of the PAT family of proteins [ 27 ], which controls the interaction between lipases and LDs; indeed, PLIN2 has been shown to be associated with lipid accumulation [ 28 ]. PLIN2 has been identified in mouse [ 29 ], porcine [ 30 ] and bovine oocytes [ 31 ] and bovine embryos [ 32 ]. In these studies, PLIN2 was shown to co-localize with LDs, indicating its role as a major LD protein in these cells.…”

Section: Introductionmentioning

confidence: 99%

The role of cGMP as a mediator of lipolysis in bovine oocytes and its effects on embryo development and cryopreservation

et al. 2018

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This study aimed to determine the influence of cyclic guanosine 3’5’-monophosphate (cGMP) and cGMP-dependent kinase (PKG) during in vitro maturation (IVM) on lipolysis-related parameters in bovine cumulus-oocyte complexes (COCs), and on embryo development and cryosurvival. COCs were matured with cGMP/PKG modulators and assessed for metaphase II rates (MII), cGMP levels, lipid content in oocytes (OO), transcript abundance for genes involved in lipolysis (ATGL) and lipid droplets (PLIN2) in cumulus cells (CC) and OO, and presence of phosphorylated (active) hormone sensitive lipase (HSLser563) in OO. Embryo development, lipid contents and survival to vitrification were also assessed. Phosphodiesterase 5 inhibition (PDE5; cGMP-hydrolyzing enzyme) with 10-5M sildenafil (SDF) during 24 h IVM increased cGMP in COCs (56.9 vs 9.5 fMol/COC in untreated controls, p<0.05) and did not affect on maturation rate (84.3±6.4% MII). Fetal calf serum (FCS) in IVM medium decreased cGMP in COCs compared to bovine serum albumin (BSA) + SDF (19.6 vs 66.5 fMol/COC, respectively, p<0.05). FCS increased lipid content in OO (40.1 FI, p<0.05) compared to BSA (34.6 FI), while SDF decreased (29.8 and 29.6 FI, with BSA or FCS, respectively p<0.05). PKG inhibitor (KT5823) reversed this effect (38.9 FI, p<0.05). ATGL and PLIN2 transcripts were detected in CC and OO, but were affected by cGMP and PKG only in CC. HSLser563 was detected in OO matured with or without modulators. Reduced lipid content in embryos were observed only when SDF was added during IVM and IVC (27.6 FI) compared to its use in either or none of the culture periods (34.2 FI, p<0.05). Survival to vitrification was unaffected by SDF. In conclusion, cGMP and PKG are involved in lipolysis in OO and possibly in CC and embryos; serum negatively affects this pathway, contributing to lipid accumulation, and cGMP modulation may reduce lipid contents in oocytes and embryos, but without improving embryo cryotolerance.

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“…Among them, Lep, a key factor in appetite, can inhibit feed intake 18, 19 and Lep mRNA was significantly decreased by the HS diet. Genes related to lipogenesis were significantly decreased in mice fed the HS diet, including those involved in fatty acid and lipid biosynthesis and transport (Scd2, Fabp5, Thrsp and Hmgcr) 20–23 , lipid endocytosis (Ldlr) 24 , and lipid esterification (Dgat2, Mogat2 and Plin2) 25–27 . Acsl6 is a negative regulator of lipid metabolism 28 .…”

Section: Discussionmentioning

confidence: 99%

High-salt intake negatively regulates fat deposition in mouse

Cui

Yang

Zheng

et al. 2017

Sci Rep

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High-salt (HS) intake contributes to hypertension and cardiopathy, but the effect of HS on fat deposition is controversial. Feed intake, fat mass, the percentage of abdominal fat, heat production, rate of oxygen consumption and the respiratory exchange ratio of mice on a HS diet were significantly decreased (P < 0.01 or 0.05) compared with mice on a normal-salt (NS) diet. An in vitro experiment with differentiating pre-adipocytes showed reduced fat deposition in the presence of high concentrations of NaCl (>0.05 M). Abdominal fat mRNA profiles and protein measurements showed that 5 known genes involved in lipolysis were up-regulated significantly and 9 genes related to lipogenesis were down-regulated in HS mice. Abundant genes and some proteins (ATP2a1, AGT, and ANGPTL4) related to calcium ion metabolism or the renin-angiotensin system (RAS) were differentially expressed between HS and NS mice. Of special interest, CREB1 phosphorylation (S133 and S142), a key factor involved in calcium signaling and other pathways, was up-regulated in HS mice. By IPA analysis, a network mediated by calcium was established providing the molecular mechanisms underlying the negative effect of HS on fat deposition.

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Expression of Perilipin 2 (PLIN2) in Porcine Oocytes During Maturation

Cited by 19 publications

References 42 publications

The consequences of porcine IVM medium supplementation with follicular fluid become reflected in embryo quality, yield and gene expression patterns

The consequences of porcine IVM medium supplementation with follicular fluid become reflected in embryo quality, yield and gene expression patterns

The role of cGMP as a mediator of lipolysis in bovine oocytes and its effects on embryo development and cryopreservation

High-salt intake negatively regulates fat deposition in mouse

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