Expression of peroxisome proliferator-activated receptor (PPARγ) mRNA in adipose and muscle tissue of Holstein and Charolais cattle

Abstract

Expression of peroxisome proliferator-activated receptor (PPARγ) mRNA in adipose and muscle tissue of Holstein and Charolais cattle. Can. J. Anim. Sci. 84: 49-55. Peroxisome proliferator-activated receptor γ (PPARγ) regulates adipogenesis and lipid metabolism-related gene transcripts. The role, however, of PPARγ in different adipose depots and muscle in Holstein and Charolais cattle is still unclear. We used 20 animals (10 from each breed) for semiquantitative reverse transcription polymerase chain reaction (RT-PCR) to measure PPARγ mRNA levels in subcutaneous (SC), perirenal (PR), omental (OM), and intramuscular (IM) adipose depots as well as longissimus muscle (MU). IM fat was dissected from muscle tissue in MU. Holstein were characterized by their higher OM (P < 0.01) and PR (P < 0.05) fat weights while the Charolais had a higher body weight (P < 0.001) and a larger longissimus muscle area (P < 0.001). The IM fat content and marbling scores tended to be higher in Holstein. No significant differences in PPARγ mRNA expression were observed between these two breeds for any tissue. In both breeds, MU PPARγ had the lowest expressed mRNA level (P < 0.05). In the IM fat depot, expression was higher (P < 0.05) than MU, but lower than the SC, PR, and OM fat depot PPARγ mRNA levels. Only OM PPARγ mRNA levels were higher (P < 0.05) than SC and PR in Charolais. To characterise the role of PPARγ mRNA in bovine adipogenesis, correlations were performed among PPARγ mRNA, carcass characteristics, and adipogenesis-related genes.

Effect of linseed addition on the expression of some lipid metabolism genes in the adipose tissue of young Italian Simmental and Holstein bulls1

“…Peroxisome proliferatoractivated receptor γ is a member of a subfamily of ligand-activated nuclear receptors that includes PPARα and PPARβ/δ (Fernyhough et al, 2007), and it plays a key role in adipocyte differentiation, fatty acid uptake, de novo fatty acid synthesis, and lipolysis and is the major regulator of lipid storage in the adipose tissue by modulating the expression of several genes involved in fatty acid metabolism (Feige et al, 2006). Results of the present study concur with those of Huff et al (2004), who did not fi nd differences in PPARγ gene expression between Charolais and Holstein cattle slaughtered at the same external fat thickness. Additionally, PPARγ2 is involved in adipose cells differentiation (Fernyhough et al, 2007), but Joseph et al (2010) suggested that the majority of adipocyte differentiation occurs within 8 mo of age in beef cattle; thus, it is plausible that the lack of an effect of bull breed on PPARγ2 expression was because bulls were greater than 8 mo of age at slaughter and the adipocyte differentiation was possibly complete.…”

Expression patterns of PPARγ2, PGC-1α, and MEF2C and their association with intramuscular fat content and skeletal muscle tenderness of crossbred Simmental bulls

“…PPARγ2 is able to promote adipocyte differentiation and lipid storage in white and brown adipose tissues (Vidal-Puig et al 1996). It is detected in buffalo (Huff et al 2004), and a positive relation is noted between mRNA abundance of PPARγ2 and IMF, which is consistent with our finding. Nevertheless, a negative association of PPARγ2 expression and IMF was noted in young sheep (Huang et al 2006) and goat (Li et al 2013) muscles, suggesting that PPARγ2 is likely involved in lipid dissipation in the skeletal muscles.…”

“…Some studies have also shown that PPARγ was an important candidate gene for IMF content in livestock and poultry (Gerbens et al 1998;Soret et al 1999;Gardan et al 2007). Polymorphisms in cattle PPARγ were found to be related to IMF content (Fan et al 2011); moreover, its expression level was positively correlated with muscle fat content in buffalo (Huff et al 2004). These studies indicate that PPARγ plays a key role in fat deposition and metabolism.…”

Functional Role of PPARs in Ruminants: Potential Targets for Fine-Tuning Metabolism during Growth and Lactation

“…In previous studies it has been shown that this PPAR isotype is expressed in bovine aortic endothelial cells [46], beef cattle skeletal muscle (including intramuscular fat) [47], ovine intramuscular fat [48], bovine perimuscular preadipocytes [49], and bovine retinal pericytes [50]. In several beef cattle breeds, PPARG had a similar degree of expression in perirenal and omental adipose depots, followed by intramuscular fat and, in a minor quantity, in the longissimus muscle [47, 51]. …”