Expression of phosphoinositide-specific phospholipase C enzymes in normal endometrium and in endometriosis

Vasco, Vincenza Rita Lo; Leopizzi, Martina; Chiappetta, Caterina; Businaro, Rita; Polonia, Patrizia; Rocca, Carlo Della; Litta, Pietro

doi:10.1016/j.fertnstert.2012.04.020

Cited by 23 publications

(25 citation statements)

References 17 publications

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“…Beside the observed effects upon cell viability, previous evidences indicated that U-73122 might act upon the gene expression of PLC isoforms (Lo Vasco et al 2010a , b , 2011 , 2012 , 2013 ). In the present experiments, molecular biology analyses indicated that a rearrangement of the panel of expression of PLC enzymes occurred after 1, 3, 6 and 24 h after U-73122 adding.…”

Section: Discussionmentioning

confidence: 94%

“…PLC δ isozymes, the most primitive and evolutionary conserved, are known to be the most sensitive to calcium (Suh et al 2008 ). Our previous studies described the variation of PLC δ1 and PLC δ3 expression in LPS-induced inflammation and inflammatory diseases, such as endometriosis (Lo Vasco et al 2010a , b , 2011 , 2012 ). That suggested that PLC δ3 might be involved in the fine tuning and regulation of the inflammation cascade.…”

Section: Discussionmentioning

confidence: 99%

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U-73122 reduces the cell growth in cultured MG-63 ostesarcoma cell line involving Phosphoinositide-specific Phospholipases C

et al. 2016

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The definition of the number and nature of the signal transduction pathways involved in the pathogenesis and the identification of the molecules promoting metastasis spread might improve the knowledge of the natural history of osteosarcoma, also allowing refine the prognosis and opening the way to novel therapeutic strategies. Phosphatydil inositol (4,5) bisphosphate (PIP2), belonging to the Phosphoinositide (PI) signal transduction pathway, was related to the regulation of ezrin, an ezrin–radixin–moesin protein involved in metastatic osteosarcoma spread. The levels of PIP2 are regulated by means of the PI-specific Phospholipase C (PLC) enzymes. Recent literature data suggested that in osteosarcoma the panel of expression of PLC isoforms varies in a complex and unclear manner and is related to ezrin, probably networking with Ras GTPases, such as RhoA and Rac1. We analyzed the expression and the subcellular localization of PLC enzymes in cultured human osteosarcoma MG-63 cells, commonly used as an experimental model for human osteoblasts, using U-73122 PLC inhibitor, U-73343 inactive analogue, and by silencing ezrin. The treatment with U-73122 significantly reduces the number of MG-63 viable cells and contemporarily modifies the expression and the subcellular localization of selected PLC isoforms. U-73122 reduces the cell growth in cultured MG-63 ostesarcoma cell line involving PI-specific Phospholipases C.

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Section: Discussionmentioning

confidence: 94%

Section: Discussionmentioning

confidence: 99%

U-73122 reduces the cell growth in cultured MG-63 ostesarcoma cell line involving Phosphoinositide-specific Phospholipases C

et al. 2016

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“…PLC enzymes are described as highly tissue-specific in normal tissues (18,19,21). However, the PLC expression panels in tissues vary under abnormal conditions compared to normal counterparts (20,21,23,24,(26)(27)(28). Moreover, the subcellular distribution of PLC enzymes seems to play Avicenna J Med Biochem.…”

Section: Discussionmentioning

confidence: 99%

“…Knowledge of the expression panels of PI-PLC enzymes is a necessary and preliminary tool to address studies about their role in quiescent cells. Moreover, the expression panels of PI-PLC enzymes have been demonstrated to differ in quiescent cells with respect to the pathological or activated counterpart (18)(19)(20)(21)(22)(23)(24)(25)(26)(27)(28)(29)(30).…”

Section: Introductionmentioning

confidence: 99%

Comparison of Phosphoinositide-Specific Phospholipase C Expression Panels of Human Osteoblasts Versus MG-63 and Saos Osteoblast-Like Cells

Vasco¹,

Leopizzi²,

d’Abusco³

et al. 2016

Avicenna J Med Biochem

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Background: A large number of phospholipase C (PLC) enzymes, both mRNA transcripts and proteins, have been detected in osteoblasts, corroborating the importance of calcium regulation in bone tissue. MG-63 and SaOS-2 human osteosarcoma cell lines are actually considered osteoblast-like cells, and are therefore widely used as experimental models for osteoblasts. Objectives: Our aim was to verify whether MG-63 or SaOS-2 cells might also represent appropriate experimental osteoblast models for signal transduction studies, with special regard to the phosphoinositide (PI) pathway. We analyzed the expression and the subcellular distribution of enzymes related to calcium signal transduction (the PI-specific PLC family), which are known to possess high cell/tissue specificity. Materials and Methods:The expression of PLC genes was analyzed by performing RT-PCR experiments. The presence of PLC enzymes and their subcellular distribution within the cells was analyzed with immunofluorescence experiments. Results: Osteoblasts, MG-63 cells, and SaOS-2 cells have expression panels similar to those of PLC enzymes. However, slight differences were found in the expression of enzymes belonging to the PLCη subfamily. Conclusions: MG-63 and SaOS-2 osteosarcoma cell lines might not represent appropriate experimental models for studies that aim to analyze signal transduction in osteoblasts.

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“…Altered folliculogenesis, poor oocyte/embryo quality, luteal phase defect, proinflammatory change of peritoneal fluid and endometrial tissue have been proposed as possible theories [5][6][7][8][9][10]. Moreover, pelvic adhesions may impair ovarian oocyte release and tubal ovum capture and transport [11].…”

Section: Introductionmentioning

confidence: 99%

Could surgeon’s expertise resolve the debate about surgery effectiveness in treatment of endometriosis-related infertility?

Gizzo

Conte

Gangi

et al. 2014

Arch Gynecol Obstet

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In patients affected by endometriosis, the choice between expectant management versus intervention should be personalized: when the estimated probability of natural conception is low, surgery may be considered as a second-line treatment. Conversely, in all other cases surgery should be offered early (as a first-line approach) as it improves the chance of spontaneous conception. The laparoscopic treatment of infertility due to endometriosis must be performed by a skilled specialized surgeon to ensure a complete "pelvic cleanout" while respecting the anatomical structures and reducing the risk of fertility impairment due to surgical procedures.

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Expression of phosphoinositide-specific phospholipase C enzymes in normal endometrium and in endometriosis

Cited by 23 publications

References 17 publications

U-73122 reduces the cell growth in cultured MG-63 ostesarcoma cell line involving Phosphoinositide-specific Phospholipases C

U-73122 reduces the cell growth in cultured MG-63 ostesarcoma cell line involving Phosphoinositide-specific Phospholipases C

Comparison of Phosphoinositide-Specific Phospholipase C Expression Panels of Human Osteoblasts Versus MG-63 and Saos Osteoblast-Like Cells

Could surgeon’s expertise resolve the debate about surgery effectiveness in treatment of endometriosis-related infertility?

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