Expression of PIN Genes in Rice ( Oryza sativa L.): Tissue Specificity and Regulation by Hormones

Abstract

Twelve genes of the PIN family in rice were analyzed for gene and protein structures and an evolutionary relationship with reported AtPINs in Arabidopsis. Four members of PIN1 (designated as OsPIN1a-d), one gene paired with AtPIN2 (OsPIN2), three members of PIN5 (OsPIN5a-c), one gene paired with AtPIN8 (OsPIN8), and three monocot-specific PINs (OsPIN9, OsPIN10a, and b) were identified from the phylogenetic analysis. Tissue-specific expression patterns of nine PIN genes among them were investigated using RT-PCR and GUS reporter. The wide variations in the expression domain in different tissues of the PIN genes were observed. In general, PIN genes are up-regulated by exogenous auxin, while different responses of different PIN genes to other hormones were found.

Osmotic stress stimulates shoot organogenesis in callus of rice (Oryza sativa L.) via auxin signaling and carbohydrate metabolism regulation

“…The auxin efflux carrier gene family, PIN-formed (PINs), is the key factor for auxin polar transport (Petrásek et al 2006;Wang et al 2009). PIN gene expression may represent auxin accumulation level (Xu et al 2006;Huang et al 2010).…”

“…PIN gene expression may represent auxin accumulation level (Xu et al 2006;Huang et al 2010). OsPIN1 is detected in rice calli (Xu et al 2006) and is related to organogenesis Wang et al 2009). Similarly, B-type response regulator (B-RR) proteins are positive signal regulators for cytokinin signaling (Müller and Sheen 2007) and the expression level of B-RR gene can be representive of endogenous cytokinin level (Mason et al 2005).…”

Microarray analysis reveals overlapping and specific transcriptional responses to different plant hormones in rice

“…The transcriptional responses of plant hormones have been analyzed at individual gene, gene family and whole genome levels in many plant species. [2][3][4][5][6][7][8][9][10][11] Based on gene expression and genetic studies in loss-of-function mutants, several genes involved in common signaling pathways of two or more hormones have also been identified and extensive crosstalk and signal integration has been uncovered. [12][13][14][15] Various genetic analyses suggested that hormones work via distinct pathways to elicit their responses.…”

Understanding the role of Oryza sativa OsPILS (PIN Like) genes in auxin signaling

“…There are twelve PIN genes present in rice and eight PIN genes in found Arabidopsis thaliana (Blilou et al 2005;Wang et al 2009a). PIN genes shows distinct pattern of cellular and sub-cellular localization in root and shoot and shows tissue specific expression Blilou et al 2005;Paponov et al 2005).…”

“…AtPIN1 localizes polarly in plasma membrane, and upon pharmacological disruption, it immediately relocalizes, suggesting conceptual basis of auxin flux affects tropic response and patterning (Estelle 1998;Friml and Palme 2002;Ikeda et al 2009;Forestan and Varotto 2012). It has reported that rice OsPIN1 gene expressed in root cap, OsPIN1b, OsPIN1c and OsPIN9 predominantly expressed in stele and OsPIN1b, OsPIN1c, OsPIN5a and OsPIN5b found to be expressed in meristem tissues (Wang et al 2009b). …”