2006
DOI: 10.1016/j.biocel.2005.10.007
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Expression of procollagen C-proteinase enhancer-1 in the remodeling rat heart is stimulated by aldosterone

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Cited by 39 publications
(37 citation statements)
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“…Kessler et al, 1990) and its expression increases in fibrosis in parallel to the characteristic increase in collagen expression (Ogata et al, 1997;Shalitin et al, 2003;Kessler-Icekson et al, 2006;Yu et al, 2013;Beaumont et al, 2014). Thus, PCPE-1 seems to function as a specific positive regulator of collagen deposition.…”
Section: Introductionmentioning
confidence: 85%
“…Kessler et al, 1990) and its expression increases in fibrosis in parallel to the characteristic increase in collagen expression (Ogata et al, 1997;Shalitin et al, 2003;Kessler-Icekson et al, 2006;Yu et al, 2013;Beaumont et al, 2014). Thus, PCPE-1 seems to function as a specific positive regulator of collagen deposition.…”
Section: Introductionmentioning
confidence: 85%
“…In the rat heart, aldosterone induces reactive fibrosis in parallel to increased reactive oxygen species (ROS) generation [43,49] possibly by enhanced procollagen-C-proteinase enhancer protein expression [17]. In addition, AT 1 or EGFRs are involved in aldosterone-induced cardiac fibrosis [29,37].…”
Section: Introductionmentioning
confidence: 99%
“…A tissue sample was homogenized and the proteins were subjected to SDS-PAGE and Western immunoblotting as previously described, loading 10 μg protein per lane (16). POPDC1 was detected by the above anti-BVES antibodies (diluted 1:200) and HRP-bovine antigoat IgG (Santa Cruz).…”
Section: Methodsmentioning
confidence: 99%
“…Total RNA was isolated (Trireagent, Sigma) and subjected to reverse transcription and quantitative polymerase chain reaction (RT-qPCR). Firststrand cDNA was synthesized in 20 μl reactions containing 1 μg total RNA, 100 U M-MLVRT (H-) reverse transcriptase (Promega, Madison, WI, USA) and 10 μM oligo(dT) [12][13][14][15][16][17][18] (17). Aliquots (0.02-1.5 μl) of each cDNA were taken for qPCR analysis (TaqMan technology, Applied Biosystems, Foster City, CA, USA) performed in the Prism 7700 Sequence Detection System.…”
Section: Methodsmentioning
confidence: 99%