Expression of RCK2 MAPKAP (MAPK-activated protein kinase) rescues yeast cells sensitivity to osmotic stress

Kumar, Vinod; Hart, A.; Wimalasena, Tithira T.; Tucker, Gregory A.; Greetham, Darren

doi:10.1186/s12934-015-0276-7

Cited by 12 publications

(9 citation statements)

References 23 publications

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“…Results here revealed that use of tetracycline-regulated system in a fermentation correlated expression of a desired gene with a phenotype; this work follows up a similar study from our previous research group [ 31 ]. Cytochrome C oxidase activity under acetic acid stress has been highlighted previously as pre-cursors for programmed cell death in yeast; however expression of COX20 correlating with acetic acid or oxidative tolerance has not been shown previously.…”

Section: Discussionsupporting

confidence: 84%

Expression of Mitochondrial Cytochrome C Oxidase Chaperone Gene (COX20) Improves Tolerance to Weak Acid and Oxidative Stress during Yeast Fermentation

et al. 2015

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Introduction Saccharomyces cerevisiae is the micro-organism of choice for the conversion of fermentable sugars released by the pre-treatment of lignocellulosic material into bioethanol. Pre-treatment of lignocellulosic material releases acetic acid and previous work identified a cytochrome oxidase chaperone gene (COX20) which was significantly up-regulated in yeast cells in the presence of acetic acid.ResultsA Δcox20 strain was sensitive to the presence of acetic acid compared with the background strain. Overexpressing COX20 using a tetracycline-regulatable expression vector system in a Δcox20 strain, resulted in tolerance to the presence of acetic acid and tolerance could be ablated with addition of tetracycline. Assays also revealed that overexpression improved tolerance to the presence of hydrogen peroxide-induced oxidative stress.ConclusionThis is a study which has utilised tetracycline-regulated protein expression in a fermentation system, which was characterised by improved (or enhanced) tolerance to acetic acid and oxidative stress.

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Section: Discussionsupporting

confidence: 84%

Expression of Mitochondrial Cytochrome C Oxidase Chaperone Gene (COX20) Improves Tolerance to Weak Acid and Oxidative Stress during Yeast Fermentation

et al. 2015

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“…Likewise, yeast eEF2 is phosphorylated by the kinase Rck2 during osmotic stress (Teige, Scheikl, Reiser, Ruis, & Ammerer, 2001). Rck2 is essential for tolerance to osmotic stress, underlining the importance of elongation regulation under these conditions (Kumar, Hart, Wimalasena, Tucker, & Greetham, 2015). Furthermore, the master regulator Hog1, which activates Rck2, is also activated by independent mechanisms during both endoplasmic reticulum (ER) stress (Bicknell, Tourtellotte, & Niwa, 2010) and oxidative stress (Lee et al, 2017), implicating elongation control by eEF2 phosphorylation under these conditions.…”

Section: Elongationmentioning

confidence: 99%

Translational regulation in response to stress in Saccharomyces cerevisiae

Crawford

Pavitt

2018

Yeast

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The budding yeast Saccharomyces cerevisiae must dynamically alter the composition of its proteome in order to respond to diverse stresses. The reprogramming of gene expression during stress typically involves initial global repression of protein synthesis, accompanied by the activation of stress-responsive mRNAs through both translational and transcriptional responses. The ability of specific mRNAs to counter the global translational repression is therefore crucial to the overall response to stress. Here we summarize the major repressive mechanisms and discuss mechanisms of translational activation in response to different stresses in S. cerevisiae. Taken together, a wide range of studies indicate that multiple elements act in concert to bring about appropriate translational responses. These include regulatory elements within mRNAs, altered mRNA interactions with RNA-binding proteins and the specialization of ribosomes that each contribute towards regulating protein expression to suit the changing environmental conditions.

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“…Another report has been obtained for radiation-induced chromosome damage by ethyl acetate fraction of A. rohituka [13]. A bioactive compound rohitukine extracted from A. rohituka possesses immune-modulatory, anti-inflammatory and anti-cancer properties, and also serves as a precursor for the synthesis of P-276-00 (Piramal Healthcare Limited, Mumbai, India) and flavopiridol, an anticancer drug [14,15]. The first study for isolation of rohitukine and its analog, rohitukine N-oxide has been demonstrated in endophytic fungi Gibberella fujikuroi MTCC 11382 obtained from A. rohituka [16].…”

Section: Introductionmentioning

confidence: 99%

Assessment of Biological Activities of Fungal Endophytes Derived Bioactive Compounds Isolated from Amoora rohituka

Verma

Gupta

Rai

et al. 2022

JoF

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Fungal endophytes have remarkable potential to produce bioactive compounds with numerous pharmacological significance that are used in various disease management and human welfare. In the current study, a total of eight fungal endophytes were isolated from the leaf tissue of Amoora rohituka, and out of which ethyl acetate (EA) extract of Penicillium oxalicum was found to exhibit potential antioxidant activity against DPPH, nitric oxide, superoxide anion and hydroxyl free radicals with EC50 values of 178.30 ± 1.446, 75.79 ± 0.692, 169.28 ± 0.402 and 126.12 ± 0.636 µg/mL, respectively. The significant antioxidant activity of EA extract of P. oxalicum is validated through highest phenolic and flavonoid content, and the presence of unique bioactive components observed through high-performance thin layer chromatography (HPTLC) fingerprinting. Moreover, EA extract of P. oxalicum also displayed substantial anti-proliferative activity with IC50 values of 56.81 ± 0.617, 37.24 ± 1.26 and 260.627 ± 5.415 µg/mL against three cancer cells HuT-78, MDA-MB-231 and MCF-7, respectively. Furthermore, comparative HPTLC fingerprint analysis and antioxidant activity of P. oxalicum revealed that fungal endophyte P. oxalicum produces bioactive compounds in a host-dependent manner. Therefore, the present study signifies that fungal endophyte P. oxalicum associated with the leaf of A. rohituka could be a potential source of bioactive compounds with antioxidant and anticancer activity.

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Expression of RCK2 MAPKAP (MAPK-activated protein kinase) rescues yeast cells sensitivity to osmotic stress

Cited by 12 publications

References 23 publications

Expression of Mitochondrial Cytochrome C Oxidase Chaperone Gene (COX20) Improves Tolerance to Weak Acid and Oxidative Stress during Yeast Fermentation

Expression of Mitochondrial Cytochrome C Oxidase Chaperone Gene (COX20) Improves Tolerance to Weak Acid and Oxidative Stress during Yeast Fermentation

Translational regulation in response to stress in Saccharomyces cerevisiae

Assessment of Biological Activities of Fungal Endophytes Derived Bioactive Compounds Isolated from Amoora rohituka

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