Expression of receptors in cow’s oviductal epithelial cells to prostaglandins E₂, D₂ and F_2α

Abstract

The significance of studying a condition of the reproductive system in productive animals, especially in dairy cattle, is directly proportional to the population’s needs in dairy, lactic acid, and other livestock products. The article presents the results of studies conducted at the Agricultural university of Hohhot city, China. The aim of the study was to identify the expression of receptors for commercial prostaglandins. The object of research was the biological material obtained from slaughter cattle, namely, the section of the cow’s oviducts. The subject of research was isolated epithelial cells. Expression was assessed by the level of intracellular Ca2+ and cAMP when exposed to PGE2, PGD2 and PGF2 of various concentrations from 10−5 to 10−9, also was using an agonist Butaprost. As a result, the concentration of PGE2 at 10−5 mol/L, PGF2α at 10−5 mol/L and 10−6 mol/L significantly contributed to an increase in the level of intracellular calcium (Ca2+) in the epithelial cells of the cow’s oviducts. The increase in the concentration of PGE2, PGD2, and Butaprost led to an increase in the level of cAMP concentration in epithelial cells of cow’s oviducts. EP1, EP2, EP4, FP, DP, TP receptors are available on cow’s oviductal epithelial cells. EP3 and IP receptors were not found on cow’s oviductal epithelial cells. Expression of the EP2, EP4, DP, FP receptor genes is well expressed, unlike EP1 and TP.