Expression of RECK and MMP-2 in salivary adenoid cystic carcinoma: Correlation with tumor progression and patient prognosis

Zhou, Xiaoqing; Huang, Shengyun; Jiang, Licheng; Li, Wengang; Chen, Zhanwei; Zhang, Dongsheng

doi:10.3892/ol.2014.1906

Cited by 23 publications

(21 citation statements)

References 26 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In a previous study, the RECK expression level was inversely correlated with the MMP9 expression level in nasopharyngeal carcinoma (27). Furthermore, there was a significant association between the positive expression of RECK and that of MMP2 in adenoid cystic carcinoma (28). These findings suggest that RECK has an important role in regulating the proliferation and migration of normal epithelial cells and carcinoma cells (29).…”

Section: Discussionmentioning

confidence: 52%

Promoter hypermethylation of the RECK gene is associated with its low expression and poor survival of esophageal squamous cell carcinoma

Zhu

Yang

et al. 2017

Oncology Letters

View full text Add to dashboard Cite

Abstract. The present study aimed to investigate the association between the methylation status of the reversion-inducing cysteine-rich protein with kazal motifs (RECK) gene and its mRNA expression levels in patients with esophageal squamous cell carcinoma (ESCC). The methylation status of RECK was analyzed by methylation-specific polymerase chain reaction (PCR), and RECK mRNA expression levels were analyzed by quantitative PCR, in 310 paired ESCC tissues. The mean RECK methylation index (MI) was 0.65 in ESCCs and 0.49 in non-tumor samples. There was a significant association between RECK methylation and the American Joint Committee on Cancer stage and lymph node metastasis in ESCC (P<0.0001; P=0.001). The mRNA expression level of RECK was lower in ESCC tissues (mean -∆Cq =-4.66) compared with non-tumor tissues (mean -∆Cq =-2.79), and decreased RECK mRNA expression levels were associated with lymph node metastasis in ESCC. In addition, RECK mRNA levels were decreased in ESCC patients with hypermethylation of the RECK gene (∆MI >0.16; mean -∆∆Cq =-2.85) compared with those with hypomethylation of the RECK gene (∆MI ≤0.16; mean -∆∆Ct =-0.83), and there was a significant difference in the mRNA expression levels of RECK between those with N 0-1 and N 2-3 lymph node metastasis (P<0.0001). A significant correlation was observed between RECK mRNA expression levels, the MI of RECK and poor postoperative survival (P=0.0003; P<0.0001). The results of the present study suggested that promoter hypermethylation may be an important factor for loss of RECK mRNA expression and may be an indicator of poor survival in ESCC.

show abstract

Section: Discussionmentioning

confidence: 52%

Promoter hypermethylation of the RECK gene is associated with its low expression and poor survival of esophageal squamous cell carcinoma

Zhu

Yang

et al. 2017

Oncology Letters

View full text Add to dashboard Cite

show abstract

“…RECK is a newly discovered tumor-suppressor gene. RECK protein has a unique inhibitory effect on the expression and activity of MMPs, as a matrix metalloproteinase inhibitor, and RECK gene expression is closely related to tumor invasion, metastasis and angiogenesis (12). Studies have shown that RECK gene expression in liver (13), pancreatic (14), breast (15) and lung cancer (16) is negatively correlated with tumor invasiveness, and patients with high RECK gene expression have a significantly more favorable prognosis than those with low expression.…”

Section: Introductionmentioning

confidence: 99%

Icariin regulates the proliferation and apoptosis of human ovarian cancer cells through microRNA-21 by targeting PTEN, RECK and Bcl-2

Jiang

Zhao

2015

Oncology Reports

View full text Add to dashboard Cite

Icariin is the main active ingredient found in the traditional Chinese medicinal plant Epimedium, and exhibits various pharmacological effects such as enhanced immune function, anticancer activity, improved cardiovascular function and endocrine adjustment. However, the effect of icariin on ovarian cancer and the related mechanism have never been investigated. In the present study, we aimed to verify whether icariin inhibits the proliferation and increases the apoptosis of human ovarian cancer cells, and its molecular mechanism in order to establish an association and identify potential therapeutic targets. In the present study, ovarian cancer A2780 cells were treated with various concentrations of icariin, and the cell viability was evaluated by 3,3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Flow cytometry and caspase-3 colorimetric assay were performed to observe apoptotic changes in the A2780 cells. qPCR analysis was used to analyze miR-21 expression in the A2780 cells. Western blot analysis was used to assess PTEN, RECK and Bcl-2 protein expression. Transfection of microRNA-21 (miR-21) and anti-miR-21 was used to investigate expression of its target genes associated with cell proliferation and apoptosis. Icariin concomitantly suppressed cell proliferation, accelerated apoptosis and increased caspase-3 activity in the A2780 cells. In the ovarian cancer A2780 cells, icariin substantially decreased the miR-21 expression level, increased PTEN and RECK protein expression levels and decreased the Bcl-2 protein expression level. Notably, miR-21 regulated the potential anticancer effects of icariin on cell proliferation and apoptosis by targeting PTEN, RECK and Bcl-2 in the ovarian cancer A2780 cells. Our results demonstrated that icariin is an excellent candidate antitumor agent which exhibits an anticancer curative effect on ovarian cancer cells. miR-21 and its target genes may play a vital role in the molecular mechanism of the anticancer effects of icariin.

show abstract

“…All ECM components can be degraded by MMPs and each element is cleaved by a specific member of the MMP family. The MMP family plays a critical role in the process of tumor cell invasion P-value Total score P-value Intensity P-value Percentage of positive cells [28][29][30]. The MMP activity is regulated at different levels, such as cellular receptors, presence of inhibitors, compartmentalization and gene expression.…”

Section: Discussionmentioning

confidence: 99%

Clinicopathologic Significance of P63, Maspin and MMP-2 Expression in Mucoepidermoid Carcinoma

Taghavi

Khozestani

Baghban

2017

J Islam Dent Assoc Iran

View full text Add to dashboard Cite

Background and Aim: Mucoepidermoid carcinoma (MEC) is the most common malignant neoplasm of the salivary glands with complex histopathologic features and clinical behavior. The aim of this study was to evaluate the clinicopathologic significance of p63, Maspin and matrix metalloproteinase (MMP)-2 expression in MEC. Materials and Methods: In this retrospective study, immunohistochemical staining was performed to detect the expression of p63, Maspin and MMP-2 in 35 cases of MEC. In addition, to detect the presence of myoepithelial differentiation, smooth muscle actin (SMA) staining was performed. Data were analyzed using non-parametric tests. Results: P63 expression, intensity of staining for p63 and MMP-2 total score showed significant correlations with histologic grade (P=0.008, P=0.035 and P=0.003, respectively). The association between p63 expression and MMP-2 total score was significant (P=0.033). P63 positive cases did not express SMA. Conclusion: Findings suggest that p63 and MMP-2 may be useful prognostic indicators in MEC. MEC is devoid of myoepithelial cells.

show abstract

Expression of RECK and MMP-2 in salivary adenoid cystic carcinoma: Correlation with tumor progression and patient prognosis

Cited by 23 publications

References 26 publications

Promoter hypermethylation of the RECK gene is associated with its low expression and poor survival of esophageal squamous cell carcinoma

Promoter hypermethylation of the RECK gene is associated with its low expression and poor survival of esophageal squamous cell carcinoma

Icariin regulates the proliferation and apoptosis of human ovarian cancer cells through microRNA-21 by targeting PTEN, RECK and Bcl-2

Clinicopathologic Significance of P63, Maspin and MMP-2 Expression in Mucoepidermoid Carcinoma

Contact Info

Product

Resources

About