Expression of Recombinant Capsid Proteins of Chitta Virus, a Genogroup II Norwalk Virus, and Development of an ELISA to Detect the Viral Antigen

Kobayashi, Shinichi; Sakae, Kenji; Suzuki, Yoshio; Ishiko, Hiroaki; Kamata, Kunio; Suzuki, Kenji; Natori, Katsuro; Miyamura, Tatsuo; Takeda, Naokazu

doi:10.1111/j.1348-0421.2000.tb02550.x

Cited by 35 publications

(20 citation statements)

References 29 publications

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“…Hyperimmune antiserum produced to VLPs is often highly specific to the homologous strain or viruses within the same genotype [80,[89][90][91][92][93]. For example, the ELISA using hyperimmune serum against NoV GII-3/Mexico strain did not detect NoV GII-4/Grimsby virus, and vice versa [90].…”

Section: Immunoassaysmentioning

confidence: 99%

Porcine enteric caliciviruses: Genetic and antigenic relatedness to human caliciviruses, diagnosis and epidemiology

Wang

Costantini

Saif

2007

Vaccine

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Porcine enteric caliciviruses include sapoviruses and noroviruses. Porcine sapoviruses infect pigs of all ages and cause diarrhea in young pigs, whereas porcine noroviruses were detected exclusively from adult pigs without clinical signs. Importantly, certain porcine norovirus strains were genetically and antigenically related to human noroviruses. This raises public health concerns that pigs may be reservoirs for emergence of epidemic human norovirus strains. This article reviews the discovery of porcine noroviruses and sapoviruses, their classification, diagnosis, epidemiology and genetic and antigenic relatedness to human caliciviruses.

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Section: Immunoassaysmentioning

confidence: 99%

Porcine enteric caliciviruses: Genetic and antigenic relatedness to human caliciviruses, diagnosis and epidemiology

Wang

Costantini

Saif

2007

Vaccine

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“…For the rNV strain 00-013, a different cloning strategy was used. PCR consisted of 30 cycles of denaturation (94°C for 30 s), primer annealing (55°C for 30 s), and extension (72°C for 60 s), using primers SRSVI-L1 BamHI (5Ј-CGGGATCCA TGATGATGGCGTCTAAGGAC-3Ј) and GI F2R SalI (5Ј-ACGCGTCGACA TCACCGGGTGTATTGTTAGG-3Ј) with the template (a PCR product amplified with primers G1F1 and G1R1 [6]). The PCR products of 00-013 were inserted into pT7 Blue, followed by sequence confirmation as described previously (10).…”

Section: Methodsmentioning

confidence: 99%

Precise Characterization of Norovirus (Norwalk-Like Virus)-Specific Monoclonal Antibodies with Broad Reactivity

Yoda¹,

Suzuki²,

Terano

et al. 2003

J Clin Microbiol

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We have been characterizing monoclonal antibodies against Norovirus (Norwalk-like virus). In the course of our study, two monoclonal antibodies generated against Norovirus genogroup II capsid protein were found to react not only to genogroup II but also to genogroup I recombinant capsid proteins. In addition, we showed that these two monoclonal antibodies reacted to a 40-amino-acid-fragment located close to the N-terminal region of genogroup II Norovirus. Similar reactivity was observed with the equivalent region of genogroup I Norovirus. In this study, we confirmed that the epitopes of the two monoclonal antibodies existed within an 11-amino-acid peptide. To obtain an idea of the reactive ranges of the two monoclonal antibodies toward different strains of Norovirus, their reactivities were investigated using 16 types of peptide constructed according to the data in GenBank and 8 recombinant capsid proteins (7 whole capsid proteins and 1 short [80-amino-acid] protein fragment). A characteristic broad reactivity of the two monoclonal antibodies is clearly shown by the results of this study. Thus, these monoclonal antibodies could be useful tools for detecting a broad range of Norovirus strains.Norwalk virus (NV) is a member of the family Caliciviridae, genus Norovirus, formerly called Norwalk-like virus and possessing a single-stranded RNA genome. NV has been a significant cause of nonbacterial infectious gastroenteritis and foodborne diseases all over the world. The virus is highly infectious and spreads through contaminated food as well as water in food-borne disease cases. In infectious gastroenteritis cases, this virus is spread from person to person within semiclosed communities such as schools, nursing homes, and hospitals. According to national food-borne disease statistics in Japan, the number of patients involved in incidents caused by NV is likely to be large. Thus, diagnosis of NV infection is extremely important for public health, since strategies for treatment of patients and control of diseases will be carried out effectively when the causative agent is diagnosed. NV has been diagnosed using electron microscopy (EM), reverse transcription-PCR (RT-PCR), and enzyme-linked immunosorbent assay (ELISA). These methods have worked efficiently in most cases. However, due to the great diversity of nucleotide sequences throughout the whole genome of NV and the capsid protein, neither ELISA nor RT-PCR detects all types of NV (3,4,5,9). In addition, the very limited numbers of particles shed in patient fecal material makes detection by EM difficult (2, 9). In cases of NV infections with homologous strains, genomic RNA detection by RT-PCR and antigen-antibody detection by ELISA can yield excellent results (1,3,4,7,8). Regardless of its variation, NV has been divided into two large genogroups, genogroup I (GI) and genogroup II (GII).We expressed a large amount of several strains of NV capsid protein in an Escherichia coli system and generated monoclonal antibodies (MAbs) against GI capsid protein (12) as well as G...

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“…A recently developed reverse transcription-PCR (RT-PCR) assay that targets the RdRp (1,2,10,16,26,27) or capsid gene (4,7,9,11,21,22,28,31,33) and phylogenetic analysis revealed that NV is classified into two genogroups, genogroup I (GI) and genogroup II (GII). In a previous study, we proposed a genotyping scheme for NV based on diversity in the capsid N terminus/shell (N/S) gene and reported nine genotypes in GI and 10 genotypes in GII (19).…”

mentioning

confidence: 99%

Coexistence of Multiple Genotypes, Including Newly Identified Genotypes, in Outbreaks of Gastroenteritis Due to Norovirus in Japan

Kageyama

Shinohara²,

Uchida³

et al. 2004

J Clin Microbiol

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Expression of Recombinant Capsid Proteins of Chitta Virus, a Genogroup II Norwalk Virus, and Development of an ELISA to Detect the Viral Antigen

Cited by 35 publications

References 29 publications

Porcine enteric caliciviruses: Genetic and antigenic relatedness to human caliciviruses, diagnosis and epidemiology

Porcine enteric caliciviruses: Genetic and antigenic relatedness to human caliciviruses, diagnosis and epidemiology

Precise Characterization of Norovirus (Norwalk-Like Virus)-Specific Monoclonal Antibodies with Broad Reactivity

Coexistence of Multiple Genotypes, Including Newly Identified Genotypes, in Outbreaks of Gastroenteritis Due to Norovirus in Japan

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