Expression of reelin in the dorsal cochlear nucleus of the mouse

Takaoka, Yuka; Setsu, Tomiyoshi; Misaki, Kazuyo; Yamauchi, Takashi; Terashima, Toshio

doi:10.1016/j.devbrainres.2005.07.014

Cited by 11 publications

(15 citation statements)

References 23 publications

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“…The pattern of reelin expression described here was similar to that found in mice [17]. Furthermore, we demonstrated direct morphological evidence for reelin-disabled-1 signaling in mature rat cochlear nucleus by investigating the distribution of disabled-1 in rat cochlear nucleus.…”

Section: Discussionsupporting

confidence: 81%

Reelin-Disabled1 signaling in the mature rat cochlear nucleus

Inagaki¹,

Ugawa

Safwat³

et al. 2009

Acta Oto-Laryngologica

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Conclusion: Immunohistochemical detection of Reelin in granular cells and disabled-1 in cochlear nucleus suggests a possible Reelin signaling pathway in mature rat cochlear nucleus. Materials and Methods: Six-week-old Wister rats were used throughout this study. The expression of reelin and disabled-1 were studied by using in situ hybridization technique and immunohistochemistry. Results: Reelin mRNA expression was observed in granular cell layer of dorsal cochlear nucleus. Immunohistochemistry using anti-reelin monoclonal antibodies confirmed reelin expression in granule cells at protein level. We also examined disabled-1 expression in cochlear nucleus and observed positive immunoreactivity in both ventricular and dorsal cochlear nucleus. In the dorsal cochlear nucleus, fusiform and cartwheel cells were labeled. In the ventricular cochlear nucleus, relatively large cells were labeled with anti-disabled-1 polyclonal antibody but the subtypes of disabled-1 positive cells could not be identified.

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Section: Discussionsupporting

confidence: 81%

Reelin-Disabled1 signaling in the mature rat cochlear nucleus

Inagaki¹,

Ugawa

Safwat³

et al. 2009

Acta Oto-Laryngologica

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“…1994). In the dorsal cochlear nucleus of reeler , neurons can migrate normally form the rhombic lip, but the lamination was not clear and the cartwheel cells and fusiform cells disperse within the nucleus and the density of each neuronal subpopulation became lower than counterparts of the wild‐type (Martin 1981; Takaoka et al. 2005).…”

Section: Discussionmentioning

confidence: 99%

Dispersion of the neurons expressing layer specific markers in the reeler brain

Dekimoto¹,

Terashima²,

Katsuyama³

2010

Dev Growth Differ

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Neurons with similar functions including neuronal connectivity and gene expression form discrete condensed structures within the vertebrate brain. This is exemplified within the circuitry formed by the cortical layers and the neuronal nuclei. It is well known that the Reelin protein is required for development of these neuronal structures in rodents and human, but the function of Reelin remains controversial. In this report, we used ''layer-specific markers'' of the cerebral cortex to carry out detailed observations of spatial distribution of the neuronal subpopulations in the brain of the Reelin deficient mouse, reeler. We observed a spatially dispersed expression of the markers in the reeler cerebral cortex. These markers are expressed also in other laminated and non-laminated structures of brain, in which we observed similar abnormal gene expression. Our observations suggest that neurons within the brain structures (such as the layers and the nuclei), which normally exhibit condensed distribution of marker expressions, loosen their segregation or scatter by a lack of Reelin.

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“…In the FCL of the DCN, many round β-gal-positive cells express CaMKIIα (Fig. 2E, arrowheads), indicating that cartwheel cells are also in the Ptf1a-lineage (Ochiishi et al, 1998;Takaoka et al, 2005). In the ML, some relatively small or mid-sized cells were double stained with β-gal and parvalbumin (Fig.…”

Section: Cn Neurons Derived From Ptf1a Neuroepithelial Domainmentioning

confidence: 99%

“…2P). We therefore retrogradely labeled giant and fusiform cells by injecting dye into the inferior colliculus (Ryugo and Willard, 1985;Ivanova and Yuasa, 1998;Takaoka et al, 2005). Fluoro-Gold was injected into the inferior colliculus of adult Ptf1a cre/+ ; R26R mice, which were sacrificed 3 days after injection, followed by immunostaining with anti-Fluoro-Gold and β-gal antibodies.…”

Section: Cn Neurons Derived From Ptf1a Neuroepithelial Domainmentioning

confidence: 99%

Inhibitory and excitatory subtypes of cochlear nucleus neurons are defined by distinct bHLH transcription factors, Ptf1a and Atoh1

et al. 2009

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The cochlear nucleus (CN), which consists of dorsal and ventral cochlear nuclei (DCN and VCN), plays pivotal roles in processing and relaying auditory information to the brain. Although it contains various types of neurons, the origins of the distinct subtypes and their developmental molecular machinery are still elusive. Here we reveal that two basic helix-loop-helix transcription factors play crucial roles in specifying neuron subtypes in the CN. Pancreatic transcription factor 1a (Ptf1a) and atonal homolog 1 (Atoh1) were found to be expressed in discrete dorsolateral regions of the embryonic neuroepithelia of the middle hindbrain (rhombomeres 2-5). Genetic lineage tracing using mice that express Cre recombinase from the Ptf1a locus or under the control of the Atoh1 promoter revealed that inhibitory (GABAergic and glycinergic) or excitatory (glutamatergic) neurons of both DCN and VCN are derived from the Ptf1a-and Atoh1-expressing neuroepithelial regions, respectively. In the Ptf1a or Atoh1 null embryos, production of inhibitory or excitatory neurons, respectively, was severely inhibited in the CN. These findings suggest that inhibitory and excitatory subtypes of CN neurons are defined by Ptf1a and Atoh1, respectively and, furthermore, provide important insights into understanding the machinery of neuron subtype specification in the dorsal hindbrain.

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Expression of reelin in the dorsal cochlear nucleus of the mouse

Cited by 11 publications

References 23 publications

Reelin-Disabled1 signaling in the mature rat cochlear nucleus

Reelin-Disabled1 signaling in the mature rat cochlear nucleus

Dispersion of the neurons expressing layer specific markers in the reeler brain

Inhibitory and excitatory subtypes of cochlear nucleus neurons are defined by distinct bHLH transcription factors, Ptf1a and Atoh1

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