Expression of reproduction and antioxidant-related genes in crayfish Cherax quadricarinatus fed with dietary feed additives

Esfahani

Annals of Animal Science

et al. 2023

The combined effects of Spirulina platensis (SP) and Pediococcus acidilactici (PA) on growth performance, digestive enzyme activity, antioxidative status, and immune genes in zebrafish were investigated in the present study. Four experimental diets were designed: control and three test diets mixed with SP at 2.5%, PA at 107 CFU/g, or a combination of 2.5% SP and 107 CFU/g PA. After 56 days, fish treated with PA and SP mixture had higher final weight, weight gain, SGR, and lower FCR than fish fed the control and SP diets (P<0.05). The results also illustrated that fish fed PA, SP, and their mixture had higher (P<0.05) protease and amylase activities than the control. The lipase activity was significantly higher in fish treated with PA or the mixture of PA and SP than in the control (P<0.05). The alternative complement pathway (ACH50) and lysozyme activity in the mucus samples of fish treated with PA or both PA and SP were significantly higher (P<0.05) than in fish treated with the control and SP diets. The total immunoglobulin level in the skin mucus was significantly higher (P<0.05) in fish fed PA than in control. In the body homogenates samples, the lysozyme activity and immunoglobulin levels were markedly higher (P<0.05) in fish treated with the mixture of PA and SP than in the control. The dietary PA and SP diet mixture improved the glutathione peroxidase, superoxide dismutase, and total antioxidative capacity. The expression of IL-1β and IL-8 genes in fish treated with PA or the mixture of PA and SP was significantly higher (P<0.05) than in fish treated with the control or SP diets. Fish treated with PA, SP, or both PA and SP had marked (P<0.05) upregulation of the lysozyme gene expression. In conclusion, the mixture of S. platensis and P. acidilactici is more effective than using each individually for improving the growth performance, digestive enzyme activity, and the immune and antioxidative capacity of zebrafish.

Section: Discussionmentioning

confidence: 99%

Combined Effects of Spirulina platensis and Pediococcus acidilactici on the Growth Performance, Digestive Enzyme Activity, Antioxidative Status, and Immune Genes in Zebrafish

Esfahani

Annals of Animal Science

et al. 2023

“…The orthogonal array design, theory, and method is a modern time-and cost-effective research technique constructing an orthogonal array to classify regions where varieties may be successfully reduced (Ross, 1996), like the previously experiments have done in plants (Elmardy et al, 2021;Liang et al, 2021;Hamedalla et al, 2022) and animals (Shehata et al, 2020(Shehata et al, , 2022. Furthermore, the interaction effects between factors can be treated as autonomous factors and evaluated using the triangular table in relation to the associated orthogonal array preserving the advantages of the traditional fractional factor design (Cortés-Jacinto et al, 2005;Shehata et al, 2022).…”

Section: Introductionmentioning

confidence: 99%

Enhancement of photosynthesis efficiency and yield of strawberry (Fragaria ananassa Duch.) plants via LED systems

et al. 2022

Due to advances in the industrial development of light-emitting diodes (LEDs), much research has been conducted in recent years to get a better understanding of how plants respond to these light sources. This study investigated the effects of different LED-based light regimes on strawberry plant development and performance. The photosynthetic pigment content, biochemical constituents, and growth characteristics of strawberry plants were investigated using a combination of different light intensities (150, 200, and 250 μmol m−2 s−1), qualities (red, green, and blue LEDs), and photoperiods (14/10 h, 16/8 h, and 12/12 h light/dark cycles) compared to the same treatment with white fluorescent light. Plant height, root length, shoot fresh and dry weight, chlorophyll a, total chlorophyll/carotenoid content, and most plant yield parameters were highest when illuminated with LM7 [intensity (250 μmol m−2 s−1) + quality (70% red/30% blue LED light combination) + photoperiod (16/8 h light/dark cycles)]. The best results for the effective quantum yield of PSII photochemistry Y(II), photochemical quenching coefficient (qP), and electron transport ratio (ETR) were obtained with LM8 illumination [intensity (250 μmol m−2 s−1) + quality (50% red/20% green/30% blue LED light combination) + photoperiod (12 h/12 h light/dark cycles)]. We conclude that strawberry plants require prolonged and high light intensities with a high red-light component for maximum performance and biomass production.

Marine and Life Sciences

“…Especially the feeds formulated for crustaceans, which, unlike fish, are known to be unable to develop acquired immunity and therefore their innate immunity should constantly be stimulated with immunostimulants (Kaya et al, 2020). In addition, feeds supplemented with such feed additives improve growth, reproduction, egg quality, and shrimp welfare (Shehata et al, 2022). Some of the functional feed additives used in aquaculture are probiotics and prebiotics, both of which are applied in this study.…”

Section: Introductionmentioning

confidence: 99%

Symbiotic effect of Bacillus clausii and Galacto-oligosaccharide on growth and survival rates in red cherry shrimp (Neocaridina davidi)

Kaya

Hersi

Genc

2022

In this 30-day experiment, the effect of probiotics (Bacillus clausii, commercial probiotics: Enterogermina) and prebiotics (Galactooligosaccharides, GOS) on the growth of red cherry shrimp (Neocaridina davidi) has been investigated for 30 days. The trial was conducted with 270 red cherry shrimps (initial weight: 0.24±0.03 g) in 27 plastic tanks (volume 0.01 m3 and area of 0.05 m2) representing 9 groups. Except for the control group, diets were supplemented with either GOS or synbiotics per kg of feed as G1 (1g GOS), G2 (2g GOS), G3 (3g GOS), G4 (4g GOS), G1P (1g GOS+ 1 ml probiotic), G2P (2 g GOS+ 1 ml probiotic), G3P (3 g GOS+ 1 ml probiotic) and G4P (4 g GOS+ 1 ml probiotic). This study showed significantly (P< 0.05) higher growth rate indices of male and female shrimps fed with feed additives groups compared to the control group. The highest final body weight, weight gain, and specific growth rate in male shrimps were recorded from the 2 g GOS+ 1 ml probiotic and 3 g GOS+ 1 ml probiotic groups. Similarly, the highest final body weight, weight gain, and specific growth rate in the female shrimps were found in the G1P and G2P synbiotic groups. Conversely, the lowest final body weight, weight gain, and specific growth rate of both the female and male groups were noted in the control groups. Regarding feed conversion ratio and survival rates of both female and male shrimps, G2P and G3P groups showed significant improvements. Regarding our study's findings, 2 g GOS and 1 ml of Bacillus clausii (commercial probiotic: Enterogermina) dietary synbiotics can be used to improve red cherry shrimp growth rates.