Expression of rice gall dwarf virus outer coat protein gene (S8) in insect cells

Fan, Guihong; Gao, Fangluan; Wèi, Tàiyún; Huang, Meiying; Wu, Zujian; Lin, Qian; Xie, Liji

doi:10.1007/s12250-010-3152-y

Cited by 4 publications

(2 citation statements)

References 15 publications

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“…In recent years, RGDV has spread to Guangdong, Hainan and Guangxi Provinces of southern China 26 . RGDV is mainly transmitted in a persistent-propagative manner by the leafhopper vector with high efficiency, and the virus has evolved to adapt to the vector 26 27 28 . Both leafhopper R. dorsalis and its cultured cells support the multiplication of RGDV to a high titer and in both, a persistent and nonlethal infection develops 26 29 30 .…”

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confidence: 99%

Small interfering RNA pathway modulates persistent infection of a plant virus in its insect vector

Lan

Wang

Chen

et al. 2016

Sci Rep

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Plant reoviruses, rhabdoviruses, tospoviruses, and tenuiviruses are transmitted by insect vectors in a persistent-propagative manner. How such persistent infection of plant viruses in insect vectors is established and maintained remains poorly understood. In this study, we used rice gall dwarf virus (RGDV), a plant reovirus, and its main vector leafhopper Recilia dorsalis as a virus–insect system to determine how the small interference (siRNA) pathway modulates persistent infection of a plant virus in its insect vector. We showed that a conserved siRNA antiviral response was triggered by the persistent replication of RGDV in cultured leafhopper cells and in intact insects, by appearance of virus-specific siRNAs, primarily 21-nt long, and the increased expression of siRNA pathway core components Dicer-2 and Argonaute-2. Silencing of Dicer-2 using RNA interference strongly suppressed production of virus-specific siRNAs, promoted viral accumulation, and caused cytopathological changes in vitro and in vivo. When the viral accumulation level rose above a certain threshold of viral genome copy (1.32 × 1014 copies/μg insect RNA), the infection of the leafhopper by RGDV was lethal rather than persistent. Taken together, our results revealed a new finding that the siRNA pathway in insect vector can modulate persistent infection of plant viruses.

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confidence: 99%

Small interfering RNA pathway modulates persistent infection of a plant virus in its insect vector

Lan

Wang

Chen

et al. 2016

Sci Rep

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“…The immunofluorescence analysis showed that P8 protein of RGDV formed punctuate structures in the cytoplasm of Sf9 cells. This system was used to overcome the insolubility of the expression products using prokaryotic expression system (Fan et al 2010). Scanning electron microscope of infected insect cells using the recombinant vAc-polh-TYLCV-CP showed geminated, icosahedral virus-like particles (VLP) typical to Begomovirus particles (20 9 30 nm).…”

Section: Discussionmentioning

confidence: 99%

Expression of tomato yellow leaf curl virus coat protein using baculovirus expression system and evaluation of its utility as a viral antigen

2017

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DNA encoding the coat protein (CP) of an Egyptian isolate of tomato yellow leaf curl virus (TYLCV) was inserted into the genome of Autographa californica nucleopolyhedrovirus (AcNPV) under the control of polyhedrin promoter. The generated recombinant baculovirus construct harboring the coat protein gene was characterized using PCR analysis. The recombinant coat protein expressed in infected insect cells was used as a coating antigen in an indirect Enzyme-linked immunosorbent assay (ELISA) and dot blot to test its utility for the detection of antibody generated against TYLCV virus particles. The results of ELISA and dot blot showed that the TYLCV-antibodies reacted positively with extracts of infected cells using the recombinant virus as a coating antigen with strong signals as well as the TYLCV infected tomato and beat plant extracts as positive samples. Scanning electron microscope examination showed that the expressed TYLCV coat protein was self-assembled into virus-like particles (VLPs) similar in size and morphology to TYLCV virus particles. These results concluded that, the expressed coat protein of TYLCV using baculovirus vector system is a reliable candidate for generation of anti-CP antibody for inexpensive detection of TYLCV-infected plants using indirect CP-ELISA or dot blot with high specificity.

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Transgenic Approaches to Develop Virus Resistance in Rice

Kumar

Dasgupta

2021

Concepts and Strategies in Plant Sciences

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Expression of rice gall dwarf virus outer coat protein gene (S8) in insect cells

Cited by 4 publications

References 15 publications

Small interfering RNA pathway modulates persistent infection of a plant virus in its insect vector

Small interfering RNA pathway modulates persistent infection of a plant virus in its insect vector

Expression of tomato yellow leaf curl virus coat protein using baculovirus expression system and evaluation of its utility as a viral antigen

Transgenic Approaches to Develop Virus Resistance in Rice

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