Expression of RING Finger Protein 38 in Serotonergic Neurons in the Brain of Nile Tilapia, Oreochromis niloticus

Cham, Kai Lin; Soga, Tomoko; Parhar, Ishwar S.

doi:10.3389/fnana.2018.00109

Cited by 9 publications

(6 citation statements)

References 74 publications

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“…Genetic variants in TDRP have been associated with attention deficit hyperactivity disorder [ 46 ]. Another of the differentially methylated genes, RNF38 , has been shown in animal studies to be regulated by the serotonin system in the brain [ 47 , 48 ], which modulates behaviors and psychological functions. Finally, the gene TTC23 , which was highlighted in our differential methylation analysis and in our analysis linking methylation to nearby gene expression, was linked to schizophrenia in a recent EWAS [ 49 ].…”

Section: Discussionmentioning

confidence: 99%

Opioid Medication Use and Blood Dna Methylation: Epigenome-Wide Association Meta-Analysis

et al. 2022

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Aim: To identify differential methylation related to prescribed opioid use. Methods: This study examined whether blood DNA methylation, measured using Illumina arrays, differs by recent opioid medication use in four population-based cohorts. We meta-analyzed results (282 users; 10,560 nonusers) using inverse-variance weighting. Results: Differential methylation (false discovery rate <0.05) was observed at six CpGs annotated to the following genes: KIAA0226, CPLX2, TDRP, RNF38, TTC23 and GPR179. Integrative epigenomic analyses linked implicated loci to regulatory elements in blood and/or brain. Additionally, 74 CpGs were differentially methylated in males or females. Methylation at significant CpGs correlated with gene expression in blood and/or brain. Conclusion: This study identified DNA methylation related to opioid medication use in general populations. The results could inform the development of blood methylation biomarkers of opioid use.

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Section: Discussionmentioning

confidence: 99%

Opioid Medication Use and Blood Dna Methylation: Epigenome-Wide Association Meta-Analysis

et al. 2022

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“…The total number of 5-HT neurons and the percentage of REST -positive 5-HT neurons were calculated. Nomenclature for the brain area was adopted from Ogawa et al ( 2016 ) and Cham et al ( 2017 , 2018 ).…”

Section: Methodsmentioning

confidence: 99%

“…In particular, social behavior in the Nile tilapia is well studied; the change in body color, relative to social rank, triggered by dynamic social interactions is an excellent feature in understanding brain chemistry changes in different social statuses (Ogawa et al, 2006 ; Lim et al, 2020 ). In tilapia brain, there are three 5-HT neuronal populations: the paraventricular organ (PVO), the dorsal and ventral periventricular pretectal nuclei (PPd and PPv), and the superior and inferior raphe (SR and IR; Cham et al, 2018 ; Higuchi et al, 2018 ).…”

Section: Introductionmentioning

confidence: 99%

Expression of Repressor Element 1 Silencing Transcription Factor (REST) in Serotonin Neurons in the Adult Male Nile Tilapia (Oreochromis niloticus)

2021

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Repressor element-1 silencing transcription factor (REST) is highly expressed in the dorsal raphe where serotonin (5-hydroxytryptamine, 5-HT) neurons are located. REST works as a transcription factor for the 5-HT receptor and tryptophan hydroxylase two-gene expression. We hypothesized that REST is co-expressed in 5-HT neurons, which, if demonstrated, would be useful to understand the mechanism of 5-HT dysfunction-related disorders such as negative emotions and depression. Therefore, the present study was designed to examine the expression of the REST gene in the brain (forebrain, midbrain, and hindbrain) of adult male Nile tilapia (Oreochromis niloticus) using rt-PCR. Besides, using immunocytochemistry, co-localization of the REST gene was examined in 5-HT neurons and with neuronal-/glial-cell markers. We found a high expression of the REST gene in the midbrain region of the dorsal raphe, an area of 5-HT neurons. Double-label immunocytochemistry showed neuron-specific expression of REST co-localized in 5-HT neurons in the dorsal and ventral parts of the periventricular pretectal nucleus, paraventricular organ, and dorsal and medial raphe nucleus. Since midbrain 5-HT neurons express REST, we speculate that REST may control 5-HT neuronal activity related to negative emotions, including depression.

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“…RNF proteins are a group of transmembrane proteins containing a unique three-dimensional domain consisting of C3HC4 amino acid residues and eight conserved cysteine and histidine residues that bind two zinc cations ( Cham et al, 2018 ). The RING domain is an important factor that enables the members of the RNF family to function as ubiquitin ligases ( Zheng and Gao, 2019 ).…”

Section: Introductionmentioning

confidence: 99%

E3 ligase RNF5 inhibits type I interferon response in herpes simplex virus keratitis through the STING/IRF3 signaling pathway

Xia

2022

Front. Microbiol.

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Herpes simplex keratitis (HSK), caused by the herpes simplex virus 1 (HSV-1), is a major blinding disease in developed countries. HSV-1 can remain latent in the host for life and cannot be eradicated. The infection causes the secretion of various cytokines and aggregation of inflammatory cells. In the early stage of inflammation, mainly neutrophils infiltrate the cornea, and CD4+ T cells mediate the immunopathological changes in herpetic stromal keratitis in the subsequent progression. The STING/IRF3-mediated type I interferon (IFN) response can effectively inhibit viral replication and control infection, but the activity of STING is affected by various ubiquitination modifications. In this study, we found that the expression of RNF5 was elevated in corneal tissues and corneal epithelial cells after infection with HSV-1. Immunofluorescence staining confirmed that RNF5 was mainly expressed in the corneal epithelial layer. We silenced and overexpressed RNF5 expression in corneal epithelial cells and then inoculated them with HSV-1. We found that the expressions of STING, p-IRF3, p-TBK1, and IFN-β mRNA increased after RNF5 silencing. The opposite results were obtained after RNF5 overexpression. We also used siRNA to silence RNF5 in the mouse cornea and then established the HSK model. Compared with the siRNA-control group, the siRNA-RNF5 group showed significantly improved corneal inflammation, reduced clinical scores and tear virus titers, and significantly increased corneal IFN-β expression. In addition, the expressions of the proinflammatory cytokines IL-6 and TNF-α in the corneal tissue were significantly decreased, indicating that RNF5 silencing could effectively promote IFN-I expression, inhibit virus replication, alleviate inflammation, and reduce corneal inflammatory damage. In summary, our results suggest that RNF5 limits the type I IFN antiviral response in HSV corneal epithelitis by inhibiting STING/IRF3 signaling.

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Expression of RING Finger Protein 38 in Serotonergic Neurons in the Brain of Nile Tilapia, Oreochromis niloticus

Cited by 9 publications

References 74 publications

Opioid Medication Use and Blood Dna Methylation: Epigenome-Wide Association Meta-Analysis

Opioid Medication Use and Blood Dna Methylation: Epigenome-Wide Association Meta-Analysis

Expression of Repressor Element 1 Silencing Transcription Factor (REST) in Serotonin Neurons in the Adult Male Nile Tilapia (Oreochromis niloticus)

E3 ligase RNF5 inhibits type I interferon response in herpes simplex virus keratitis through the STING/IRF3 signaling pathway

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