Expression of S-100 protein in renal cell neoplasms

Lin, Fan; Yang, Wannian; Betten, Mark W.; Teh, Bin Tean; Yang, Ximing J.

doi:10.1016/j.humpath.2005.12.008

Cited by 52 publications

(42 citation statements)

References 37 publications

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“…[28][29][30][31][32][33][34][35][36][37][38] Renal cell carcinoma marker antibody, CD10, glutathione S-transferase-a and others have shown specificity for clear cell renal cell carcinoma, 28,29 a-Methylacyl CoA racemase is expressed in papillary renal cell carcinoma, 30 whereas parvalbumin, b-defensin 1 and most recently KIT are used as markers for chromophobe renal cell carcinoma and oncocytoma. [31][32][33] However a marker with sufficient specificity to differentiate between chromophobe renal cell carcinoma and oncocytomas has not yet been unequivocally established.…”

Section: Discussionmentioning

confidence: 99%

“…36 Inversely, RON proto-oncogene product and S100 protein were found preferentially expressed in oncocytoma warranting further investigation. 37,38 Preclinical studies have demonstrated a developmental expression of claudin isoforms in the renal tissue, 39 and have identified a distinct pattern of expression of isoforms 7 and 8 along the mouse distal nephron. 10 Gene expression profiling studies in humans have proposed in recent years that chromophobe renal cell carcinoma and oncocytoma are distinguishable by mRNA expression profiles and have indicated claudin 7 as one of the candidate markers for chromophobe renal cell carcinoma.…”

Section: Discussionmentioning

confidence: 99%

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The diagnostic and prognostic utility of claudin expression in renal cell neoplasms

et al. 2008

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This study evaluated the expression patterns of claudins 1, 3, 4, 7, and 8 in human renal cell carcinomas and oncocytomas and correlated expression with patient prognosis. Tissue microarrays were created from paraffinembedded tissue samples from 141 patients with renal cell carcinomas or oncocytoma (90 clear cell, 22 papillary, 17 chromophobe renal cell carcinomas, and 12 oncocytomas). The staining pattern for claudins 3, 4, 7, and 8 was membranous and/or cytoplasmic, whereas claudin 1 was predominantly membranous in both nonneoplastic renal tissue and tumors. Negative to weak claudin 3 staining was predominantly detected in Fuhrman's grade 1 and 2 clear cell renal cell carcinomas (78%; P ¼ 0.016), suggesting that upregulation of claudin 3 potentially occurs concomitantly with increasing grade of clear cell renal cell carcinomas. In addition, Kaplan-Meier univariate analysis showed a significant inverse correlation between moderate to strong claudin 3 and 4 expression with overall survival in clear cell renal cell carcinomas (P ¼ 0.038 and P ¼ 0.031). Moderate to strong claudin 7 expression was significantly more common in chromophobe renal cell carcinomas (94%) than in oncocytomas (55%; P ¼ 0.041). Claudin 8 staining was moderate to strong in 92% of oncocytomas, which differentiated them from papillary and clear cell renal cell carcinomas (14 and 12%; both Po0.0001). Only negative to weak claudin 8 staining was detected in all chromophobe renal cell carcinomas, whereas there were no claudin 8 negative oncocytomas and 8% exhibited a weak staining pattern (Po0.0001). Due to their distinctive expression patterns, claudins 7 and 8 can be used as useful immunohistochemical markers for the separation of chromophobe renal cell carcinomas from oncocytomas, whereas claudins 3 and 4 may serve as indicators of prognosis in clear cell renal cell carcinomas.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

The diagnostic and prognostic utility of claudin expression in renal cell neoplasms

et al. 2008

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“…This parallels the observation that chromophobe renal cell carcinoma, in which the level of S100A1 mRNA expression is extremely low, showed immunohistochemical negativity for S100A1. Lin et al 17 reported the immunohistochemical detection of S100 protein in 87% (13/15) of oncocytomas, with strong and diffuse staining in 60% of cases, whereas only one (6%) of 16 chromophobe renal cell carcinomas showed focal positivity. They used a rabbit polyclonal antibody for S100 protein that has been shown to react with both human and cow S100 protein, with a stronger reaction to S100B, but a weak reaction to S100A1 and S100A6.…”

Section: Discussionmentioning

confidence: 99%

“…They used a rabbit polyclonal antibody for S100 protein that has been shown to react with both human and cow S100 protein, with a stronger reaction to S100B, but a weak reaction to S100A1 and S100A6. 17 In our study, we used a monoclonal mouse antihuman antibody specifically recognizing S100A1 protein.…”

Section: Discussionmentioning

confidence: 99%

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Diagnostic utility of S100A1 expression in renal cell neoplasms: an immunohistochemical and quantitative RT-PCR study

et al. 2007

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S100A1 is a calcium-binding protein, which has been recently found in renal cell neoplasms. We evaluated the diagnostic utility of immunohistochemical detection of S100A1 in 164 renal cell neoplasms. Forty-one clear cell, 32 papillary, and 51 chromophobe renal cell carcinomas, and 40 oncocytomas, 164 samples of normal renal parenchyma adjacent to the tumors and 13 fetal kidneys were analyzed. The levels of S100A1 mRNA detected by quantitative RT-PCR analysis of frozen tissues from seven clear cell, five papillary, and six chromophobe renal cell carcinomas, four oncocytomas, and nine samples of normal renal tissues adjacent to neoplasms were compared with the immunohistochemical detection of protein expression. Clear cell and papillary renal cell carcinomas showed positive reactions for S100A1 in 30 out of 41 tumors (73%) and in 30 out of 32 (94%) tumors, respectively. Thirty-seven renal oncocytomas out of 40 (93%) were positive for S100A1, whereas 48 of 51 (94%) chromophobe renal cell carcinomas were negative. S100A1 protein was detected in all samples of unaffected and fetal kidneys. S100A1 mRNA was detected by RT-PCR in all normal kidneys and renal cell neoplasms, although at very different levels. Statistical analyses comparing the different expression of S100A1 in clear cell and chromophobe renal cell carcinomas observed by immunohistochemical and RT-PCR methods showed significant values (Po0.001), such as when comparing by both techniques the different levels of S100A1 expression in chromophobe renal cell carcinomas and oncocytomas (Po0.001). Our study shows that S100A1 protein is expressed in oncocytomas, clear cell and papillary renal cell carcinomas but not in chromophobe renal cell carcinomas. Its immunodetection is potentially useful for the differential diagnosis between chromophobe renal cell carcinoma and oncocytoma. Further, S100A1 protein expression is constantly detected in the normal parenchyma of the adult and fetal kidney.

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Renal Manifestations of Tuberous Sclerosis Complex

Bissler

Henske

2010

Tuberous Sclerosis Complex

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Expression of S-100 protein in renal cell neoplasms

Cited by 52 publications

References 37 publications

The diagnostic and prognostic utility of claudin expression in renal cell neoplasms

The diagnostic and prognostic utility of claudin expression in renal cell neoplasms

Diagnostic utility of S100A1 expression in renal cell neoplasms: an immunohistochemical and quantitative RT-PCR study

Renal Manifestations of Tuberous Sclerosis Complex

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