Expression of scinderin in megakaryoblastic leukemia cells induces differentiation, maturation, and apoptosis with release of plateletlike particles and inhibits proliferation and tumorigenesis

Global Effects of BCR/ABL and TEL/PDGFRβ Expression on the Proteome and Phosphoproteome

“…Their activity can be modulated by tropomyosin, which anneals gelsolin-capped actin and forms long actin filaments (35). The expression of adseverin in a megakaryoblastic leukemia model induces differentiation and inhibits proliferation and transforming ability (55). Arp2 also has a role in the formation of short, branched actin filaments in motile cells (34).…”

Identification of Stage-Specific Gene Modulation during Early Thymocyte Development by Whole-Genome Profiling Analysis after Aryl Hydrocarbon Receptor Activation

“…The elevated thymus expression of scinderin after AHR activation identifies this protein as an interesting candidate potentially involved in mediating TCDD-induced changes in T-cell development and cell cycle regulation. Indeed, loss of scinderin expression has been associated with megakaryoblastic leukemia cells, and the forced expression of scinderin within these oncogenic cells dramatically reduced cell proliferation (Zunino et al, 2001). The decrease in the megakaryocyte proliferation was attributed, at least in part, to a loss of filamentous actin, which is important for cell division (Zunino et al, 2001).…”

“…Indeed, loss of scinderin expression has been associated with megakaryoblastic leukemia cells, and the forced expression of scinderin within these oncogenic cells dramatically reduced cell proliferation (Zunino et al, 2001). The decrease in the megakaryocyte proliferation was attributed, at least in part, to a loss of filamentous actin, which is important for cell division (Zunino et al, 2001). Given this potential cell-cycle regulatory role of scinderin, the earlier identification and our confirmation of scinderin expression in immature thymocytes after AHR activation raises the hypothesis that AHR activation in stage TN3 and TN4 thymocytes induces scinderin, leading to a decrease in filamentous actin and a rapid loss of cell proliferation.…”

Gene Expression Analysis during Platelet-Like Particle Production in Phorbol Myristate Acetate-Treated MEG-01 Cells

“…MEG-01 cells, a human megakaryocytic leukemia cell line, 13) is a popular cell line that has been used in megakaryocyte differentiation studies, in gene function validation studies in megakaryocytopoiesis, in studies of PLT-like particle production, ploidy, and for the study of in vivo tumorigenesis. 14) It has been confirmed that MEG-01 cells are committed to the megakaryocytic lineage and can produce PLT-like particles. 15) In addition, it was reported that treatment with phorbol myristate acetate (PMA) enhanced the differentiation of MEG-01 cells, 16) leading to the possibility that PMA-treatment could enhance the production of PLT-like particles from the MEG-01 cells.…”