Expression of<scp>l</scp>-Selectin (CD62L), CD44, and CD25 on Activated Bovine T Cells

Waters, W. Ray; Rahner, T. E.; Palmer, M. V.; Cheng, Danny; Nonnecke, Brian J.; Whipple, Diana L.

doi:10.1128/iai.71.1.317-326.2003

Cited by 70 publications

(66 citation statements)

References 55 publications

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“…In the current study, we show a reduction in CD62L expression that is most pronounced 2 d following infection, followed by a rapid recovery of CD62L expression, similar to what has been reported during bovine leukemia virus (26) or Mycobacterium bovis (27) infection. In contrast to our finding in this viral infection, Walcheck and Jutila (28) found a much slower downregulation of L-selectin following short-term activation of gd T cells in vitro with PMA.…”

Section: Discussionsupporting

confidence: 63%

Rapid and Transient Activation of γδ T Cells to IFN-γ Production, NK Cell-Like Killing, and Antigen Processing during Acute Virus Infection

Toka

Kenney

Golde

2011

The Journal of Immunology

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γδ T cells are the majority peripheral blood T cells in young cattle. The role of γδ T cells in innate responses against infection with foot-and-mouth disease virus was analyzed on consecutive 5 d following infection. Before infection, bovine WC1+ γδ T cells expressed a nonactivated phenotype relative to CD62L, CD45RO, and CD25 expression and did not produce IFN-γ ex vivo. Additionally, CD335 expression was lacking and no spontaneous target cell lysis could be detected in vitro, although perforin was detectable at a very low level. MHC class II and CD13 expression were also lacking. Following infection with foot-and-mouth disease virus, expression of CD62L and CD45RO was greatly reduced on WC1+ γδ T cells, and unexpectedly, CD45RO expression did not recover. A transient increase in expression of CD25 correlated with production of IFN-γ. Expression of CD335 and production of perforin were detected on a subset of γδ T cells, and this correlated with an increased spontaneous killing of xenogeneic target cells. Furthermore, increased MHC class II expression was detected on WC1+ γδ T cells, and these cells processed protein Ags. These activities are rapidly induced, within 3 d, and wane by 5 d following infection. All of these functions, NK-like killing, Ag processing, and IFN-γ production, have been demonstrated for these cells in various species. However, these results are unique in that all these functions are detected in the same samples of WC1+ γδ T cells, suggesting a pivotal role of these cells in controlling virus infection.

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Section: Discussionsupporting

confidence: 63%

Rapid and Transient Activation of γδ T Cells to IFN-γ Production, NK Cell-Like Killing, and Antigen Processing during Acute Virus Infection

Toka

Kenney

Golde

2011

The Journal of Immunology

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“…It is generally accepted that extravasation and trafficking of activated lymphocytes to infected tissues require down regulation of CD62L expression [6]. Accordingly, loss of CD62L expression on T-cell upon activation was demonstrated in cattle infected with Mycobacterium bovis and respiratory syncytial virus both in vivo and in vitro after antigenic stimulation [13,25]. In CBPP, CFSE loading indicated that in most animals both CD62L + and CD62L…”

Section: Discussionmentioning

confidence: 99%

“…Initial experiments focused on the kinetics of the in vitro recall response to MmmSC by analyzing changes in frequencies and numbers of major lymphocyte subsets. In addition, the effect of antigenic stimulation on the expression of CD25 and CD62L, two indicators of pathogendriven lymphocyte activation in cattle [13,25], and on the expression of the memory marker CD45RO, was evaluated. Proliferation among major lymphocyte subsets was analyzed further by the CFSE based method on optimal time-points.…”

Section: Introductionmentioning

confidence: 99%

Analysis of cellular responses toMycoplasma mycoidessubsp.mycoidessmall colony biotype associated with control of contagious bovine pleuropneumonia

Totté

Rodrigues

YaYa³

et al. 2007

Vet. Res.

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-A better understanding of protective immune memory against contagious bovine pleuropneumonia (CBPP) is needed in order to facilitate the development of safer vaccines based on selected components of the pathogen. For this purpose, cells collected from lymph nodes draining the lungs of Mycoplasma mycoides subsp. mycoides small colony biotype (MmmSC)-infected cattle were stimulated with the pathogen in vitro and evaluated concurrently for proliferation (CFSE based method), expression of activation, memory markers and cytokine production. Direct evidence is presented for a major contribution of CD4 + T cells to the vigorous proliferative and T1 biased cytokine recall responses observed in cattle that have recovered from infection but not in animals developing the acute form of the disease. Two different phenotypes of MmmSC-specific memory CD4 were observed based on CD62L expression and proliferative capacities. Furthermore, recall proliferation of B cells also occurred but was strictly dependent on the presence of CD4. The information provided in this study will facilitate the search for MmmSC antigens that have potential for the development of subunit vaccines against CBPP.contagious bovine pleuropneumonia / Mycoplasma mycoides subsp. mycoides SC / vaccine / memory / CD4

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“…However, it is downregulated upon T cell activation, resulting in the migration of T cells to the inflammatory site (29). Through rolling and adhesion within the circulatory system, naive T cells are decelerated by CD62L binding with peripheral lymph node vascular addressins (GlyCAM-1 and MAdCAM-1) expressed on the face of the lumen of high endothelial venules.…”

Section: Discussionmentioning

confidence: 99%

Flt3-L Increases CD4⁺CD25⁺Foxp3⁺ICOS⁺Cells in the Lungs of Cockroach-Sensitized and -Challenged Mice

McGee¹,

Edwan²,

Agrawal³

2010

Am J Respir Cell Mol Biol

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We previously reported in an ovalbumin-induced model of allergic asthma that Fms-like tyrosine kinase 3 ligand (Flt3-L) reversed airway hyperresponsiveness (AHR) and airway inflammation, and increased the number of regulatory CD11c high CD8a high CD11b low dendritic cells in the lung. In this study, we investigated the effect of Flt3-L in a clinically relevant aeroallergen-induced asthma on the phenotypic expression of lung T cells. Balb/c mice were sensitized and challenged with cockroach antigen (CRA), and AHR to methacholine was established. These mice received three intraperitoneal injections of anti-CD25 antibody (PC61; 250 mg) and Flt3-L (3 mg) daily for 10 days. Cytokines and Ig levels in the serum were measured and differential bronchoalveolar lavage fluid (BALF) cell counts were examined. Flt3-L reversed AHR to methacholine to the control level. Flt3-L significantly decreased levels of BALF IL-5, IFN-g, eosinophilia and substantially increased IL-10 and the number of CD4 1 CD25 1 Forkhead winged helix transcription factor box P3 (Foxp3 1 ) IL-10 1 T cells in the lung. Administration of PC61 antibody blocked the effect of Flt3-L and substantially increased AHR, eosinophilia, and BALF IL-5 and IFN-g levels, and decreased BALF IL-10 levels and the number of CD4 1 CD25 1 Foxp3 1 IL-10 1 T cells. Flt3-L significantly decreased CD62-L, but increased inducible costimulatory molecule and Foxp3 mRNA expression in the CD4 1 CD25 1 T cells isolated from lungs of Flt3-L-treated, CRA-sensitized mice compared to CRA-sensitized mice without Flt3-L treatment and PBS control group. Flt3-L significantly inhibited the effect of CRA sensitization and challenge to increase GATA3 expression in lung CD4 1 CD25 1 T cells. Collectively, these data suggest that the therapeutic effect of Flt3-L is mediated by increased density of naturally occurring CD4 1 CD25 1 Foxp3 1 IL-10 1 ICOS 1 T-regulatory cells in the lung. Flt3-L could be a therapeutic strategy for the management and prevention of allergic asthma.

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Expression ofl-Selectin (CD62L), CD44, and CD25 on Activated Bovine T Cells

Cited by 70 publications

References 55 publications

Rapid and Transient Activation of γδ T Cells to IFN-γ Production, NK Cell-Like Killing, and Antigen Processing during Acute Virus Infection

Rapid and Transient Activation of γδ T Cells to IFN-γ Production, NK Cell-Like Killing, and Antigen Processing during Acute Virus Infection

Analysis of cellular responses toMycoplasma mycoidessubsp.mycoidessmall colony biotype associated with control of contagious bovine pleuropneumonia

Flt3-L Increases CD4⁺CD25⁺Foxp3⁺ICOS⁺Cells in the Lungs of Cockroach-Sensitized and -Challenged Mice

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Expression ofl-Selectin (CD62L), CD44, and CD25 on Activated Bovine T Cells

Cited by 70 publications

References 55 publications

Rapid and Transient Activation of γδ T Cells to IFN-γ Production, NK Cell-Like Killing, and Antigen Processing during Acute Virus Infection

Rapid and Transient Activation of γδ T Cells to IFN-γ Production, NK Cell-Like Killing, and Antigen Processing during Acute Virus Infection

Analysis of cellular responses toMycoplasma mycoidessubsp.mycoidessmall colony biotype associated with control of contagious bovine pleuropneumonia

Flt3-L Increases CD4+CD25+Foxp3+ICOS+Cells in the Lungs of Cockroach-Sensitized and -Challenged Mice

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Flt3-L Increases CD4⁺CD25⁺Foxp3⁺ICOS⁺Cells in the Lungs of Cockroach-Sensitized and -Challenged Mice