Expression of <scp>MSTN</scp>/Smad signaling pathway genes and its association with meat quality in Tibetan sheep (<i>Ovis aries</i>)

He, Na; Xia, Lang; Wang, Cailian; Lv, Cailing; Li, Mingming; Sun, Ruizhe; Zhang, Junxia

doi:10.1002/fsn3.3216

Cited by 3 publications

(2 citation statements)

References 50 publications

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“…We speculated that the miR-10 family is tightly regulated by muscle fiber transformation. The target genes of the miR-10 family in the ceRNA network included DES [ 20 ], MYBPH [ 21 ] and SMAD3 [ 22 ]. To verify this hypothesis, we constructed MYBPH wild-type (WT) plasmids and mutantion (MUT) plasmids for co-transfection with miR-10a-5p mimics, and dual luciferase reporter genes determined that miR-10a-5p and MYBPH had strong binding ability (Fig.…”

Section: Resultsmentioning

confidence: 99%

Identification of different myofiber types in pigs muscles and construction of regulatory networks

Li,

Wang,

Sun

et al. 2024

BMC Genomics

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Background Skeletal muscle is composed of muscle fibers with different physiological characteristics, which plays an important role in regulating skeletal muscle metabolism, movement and body homeostasis. The type of skeletal muscle fiber directly affects meat quality. However, the transcriptome and gene interactions between different types of muscle fibers are not well understood. Results In this paper, we selected 180-days-old Large White pigs and found that longissimus dorsi (LD) muscle was dominated by fast-fermenting myofibrils and soleus (SOL) muscle was dominated by slow-oxidizing myofibrils by frozen sections and related mRNA and protein assays. Here, we selected LD muscle and SOL muscle for transcriptomic sequencing, and identified 312 differentially expressed mRNA (DEmRs), 30 differentially expressed miRNA (DEmiRs), 183 differentially expressed lncRNA (DElRs), and 3417 differentially expressed circRNA (DEcRs). The ceRNA network included ssc-miR-378, ssc-miR-378b-3p, ssc-miR-24-3p, XR_308817, XR_308823, SMIM8, MAVS and FOS as multiple core nodes that play important roles in muscle development. Moreover, we found that different members of the miR-10 family expressed differently in oxidized and glycolytic muscle fibers, among which miR-10a-5p was highly expressed in glycolytic muscle fibers (LD) and could target MYBPH gene mRNA. Therefore, we speculate that miR-10a-5p may be involved in the transformation of muscle fiber types by targeting the MYHBP gene. In addition, PPI analysis of differentially expressed mRNA genes showed that ACTC1, ACTG2 and ACTN2 gene had the highest node degree, suggesting that this gene may play a key role in the regulatory network of muscle fiber type determination. Conclusions We can conclude that these genes play a key role in regulating muscle fiber type transformation. Our study provides transcriptomic profiles and ceRNA interaction networks for different muscle fiber types in pigs, providing reference for the transformation of pig muscle fiber types and the improvement of meat quality.

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Section: Resultsmentioning

confidence: 99%

Identification of different myofiber types in pigs muscles and construction of regulatory networks

Li,

Wang,

Sun

et al. 2024

BMC Genomics

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“…Polyunsaturated fatty acids have been shown to affect energy metabolism in liver and fat cells, leading to reduced fat accumulation [ 35 ]. Similarly, according to He et al, it was found that reduced MSTN resulted in increased levels of polyunsaturated fatty acids, especially arachidonic acid, in rams [ 41 ]. Consequently, the increased content of polyunsaturated fatty acids such as arachidonic acid and linoleic acid in our study may contribute to the observed decrease in fat deposition resulting from MSTN mutation.…”

Section: Discussionmentioning

confidence: 99%

The Effect of MSTN Mutation on Bile Acid Metabolism and Lipid Metabolism in Cattle

Wang

Hai

et al. 2023

Metabolites

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Myostatin (MSTN) is a negative regulator of skeletal muscle genesis during development. MSTN mutation leads to increased lean meat production and reduced fat deposition in livestock. However, the mechanism by which MSTN promotes myogenesis by regulating metabolism is not clear. In this study, we compared the metabolomics of the livers of wild-type (WT) and MSTN mutation cattle (MT), and found changes in the content and proportion of fatty acids and bile acids in MT cattle. The differential metabolites were enriched in sterol synthesis and primary bile acid synthesis. We further analyzed the expression of genes involved in the regulation of lipid and bile acid metabolism, and found that the loss of MSTN may alter lipid synthesis and bile acid metabolism. This study provides new basic data for MSTN mutations in beef cattle breeding.

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Integration of transcriptome and machine learning to identify the potential key genes and regulatory networks affecting drip loss in pork

Yang,

Hou,

Wang

et al. 2024

Journal of Animal Science

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Low level of drip loss (DL) is an important quality characteristic of meat with high economic value. However, the key genes and regulatory networks contributing to DL in pork remain largely unknown. To accurately identify the key genes affecting DL in muscles postmortem, 12 Duroc × (Landrace × Yorkshire) pigs with extremely high (n = 6, H group) and low (n = 6, L group) DL at both 24 and 48 h postmortem were selected for transcriptome sequencing. The analysis of differentially expressed genes and weighted gene co-expression network analysis (WGCNA) were performed to find the overlapping genes using the transcriptome data, and functional enrichment and protein–protein interaction (PPI) network analysis were conducted using the overlapping genes. Moreover, we used machine learning to identify the key genes and regulatory networks related to DL based on the interactive genes of the PPI network. Finally, nine potential key genes (IRS1, ESR1, HSPA6, INSR, SPOP, MSTN, LGALS4, MYLK2, and FRMD4B) mainly associated with the MAPK signaling pathway, the insulin signaling pathway, and the calcium signaling pathway were identified, and a single-gene set enrichment analysis (GSEA) was performed to further annotate the functions of these potential key genes. The GSEA results showed that these genes are mainly related to ubiquitin-mediated proteolysis and oxidative reactions. Taken together, our results indicate that the potential key genes influencing DL are mainly related to insulin signaling mediated differences in glycolysis and ubiquitin-mediated changes in muscle structure and improve the understanding of gene expression and regulation related to DL and contribute to future molecular breeding for improving pork quality.

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Expression of MSTN/Smad signaling pathway genes and its association with meat quality in Tibetan sheep (Ovis aries)

Cited by 3 publications

References 50 publications

Identification of different myofiber types in pigs muscles and construction of regulatory networks

Identification of different myofiber types in pigs muscles and construction of regulatory networks

The Effect of MSTN Mutation on Bile Acid Metabolism and Lipid Metabolism in Cattle

Integration of transcriptome and machine learning to identify the potential key genes and regulatory networks affecting drip loss in pork

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