Expression of Serum Amyloid A, in Normal, Dysplastic, and Neoplastic Human Colonic Mucosa: Implication for a Role in Colonic Tumorigenesis

Gutfeld, Orit; Prus, Diana; Ackerman, Zvi; Dishon, Shira; Linke, Reinhold P.; Levin, Mark; Urieli-Shoval, Simcha

doi:10.1369/jhc.5a6645.2005

Cited by 80 publications

(91 citation statements)

References 43 publications

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“…Using in situ hybridisation and IHC, they demonstrated the local and the differential expressions of SAA in human colon cancer tissues when compared with normal colonic mucosa. Furthermore they showed progressively higher SAA positivity through the different stages of dysplasia to overt carcinoma (Gutfeld et al, 2006). These findings in human colon carcinoma combined with our results in USPC seem to suggest a novel role for SAA autocrine production in colonic and endometrial tumorigenesis.…”

Section: Healthysupporting

confidence: 80%

“…The anti-SAA monoclonal antibody used (i.e., clone mcl, DAKO), was directed against SAA 1. The preparation and specificity of this antibody has been previously described and demonstrated (Gutfeld et al, 2006). Colour was developed using AEC substrate (Zymed Laboratories Inc., South San Francisco, CA, USA) for 10 min followed by counterstaining with haematoxylin.…”

Section: Saa Immunostaining Of Formalin-fixed Tumour Tissuesmentioning

confidence: 99%

“…More importantly, these results support our hypothesis that, in USPC patients, SAA is not only a liver-secreted protein but is also a USPC cell product. Of interest, Gutfeld et al (2006) have recently reported on SAA expression in normal, dysplastic, and neoplastic colonic mucosa. Using in situ hybridisation and IHC, they demonstrated the local and the differential expressions of SAA in human colon cancer tissues when compared with normal colonic mucosa.…”

Section: Healthymentioning

confidence: 99%

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Serum amyloid A (SAA): a novel biomarker for uterine serous papillary cancer

et al. 2009

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BACKGROUND: Uterine serous papillary carcinoma (USPC) is a biologically aggressive variant of endometrial cancer. We investigated the expression of Serum Amyloid A (SAA) and evaluated its potential as a serum biomarker in USPC patients. METHODS: SAA gene and protein expression levels were evaluated in USPC and normal endometrial tissues (NEC) by real-time PCR, immunohistochemistry (IHC), flow cytometry and by a sensitive bead-based immunoassay. SAA concentration in 123 serum samples from 51 healthy women, 42 women with benign diseases, and 30 USPC patients were also studied. RESULTS: SAA gene expression levels were significantly higher in USPC when compared with NEC (mean copy number by RT -PCR ¼ 162 vs 2.21; P ¼ 0.0002). IHC revealed diffuse cytoplasmic SAA protein staining in USPC tissues. High intracellular levels of SAA were identified in primary USPC cell lines evaluated by flow cytometry and SAA was found to be actively secreted in vitro. SAA concentrations (mg ml À1 ) had a median (95% CIs) of 6.0 (4.0 -8.9) in normal healthy females and 6.0 (4.2 -8.1) in patients with benign disease (P ¼ 0.92). In contrast, SAA values in the serum of USPC patients had a median (95% CI) of 15.6 (9.2 -56.2), significantly higher than those in the healthy group (P ¼ 0.0005) and benign group (P ¼ 0.0006). Receiver operating characteristics (ROC) analysis of serum SAA to classify advanced-and early-stage USPC yielded an area under the ROC curve of 0.837 (P ¼ 0.0024). CONCLUSION: SAA is not only a liver-secreted protein but is also a USPC cell product. SAA may represent a novel biomarker for USPC to assist in staging patients preoperatively, and to monitor early-disease recurrence and response to therapy.

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Section: Healthysupporting

confidence: 80%

Section: Saa Immunostaining Of Formalin-fixed Tumour Tissuesmentioning

confidence: 99%

Section: Healthymentioning

confidence: 99%

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Serum amyloid A (SAA): a novel biomarker for uterine serous papillary cancer

et al. 2009

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“…predominantly by epithelial cells and macrophages (27)(28)(29). CCL21, a chemokine expressed mainly in lymph nodes, has a critical role in the homing of T cells to these organs (30,31).…”

Section: Discussionmentioning

confidence: 99%

Gene Expression Patterns in the Human Breast after Pregnancy

Asztalos

Gann

Hayes

et al. 2010

Cancer Prevention Research

118

117

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Epidemiologic studies have established that pregnancy has a bidirectional, time-dependent effect on breast cancer risk; a period of elevated risk is followed by a long-term period of protection. The purpose of the present study was to determine whether pregnancy and involution are associated with gene expression changes in the normal breast, and whether such changes are transient or persistent. We examined the expression of a customized gene set in normal breast tissue from nulliparous, recently pregnant (0-2 years since pregnancy), and distantly pregnant (5-10 years since pregnancy) age-matched premenopausal women. This gene set included breast cancer biomarkers and genes related to immune/inflammation, extracellular matrix remodeling, angiogenesis, and hormone signaling. Laser capture microdissection and RNA extraction were done from formalin-fixed paraffin-embedded reduction mammoplasty and benign biopsy specimens and analyzed using real-time PCR arrays containing 59 pathway-specific and 5 housekeeping genes. We report 14 of 64 (22%) of the selected gene set to be differentially regulated (at P < 0.05 level) in nulliparous versus parous breast tissues. Based on gene set analysis, inflammationassociated genes were significantly upregulated as a group in both parous groups compared with nulliparous women (P = 0.03). Moreover, parous subjects had significantly reduced expression of estrogen receptor α (ERα, ESR1), progesterone receptor (PGR), and ERBB2 (Her2/neu) and 2-fold higher estrogen receptor-β (ESR2) expression compared with nulliparous subjects. These initial data, among the first on gene expression in samples of normal human breast, provide intriguing clues about the mechanisms behind the time-dependent effects of pregnancy on breast cancer risk. Cancer Prev Res; 3(3); 301-11. ©2010 AACR.

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“…There are several accepted screening methods, including fecal occult blood testing, sigmoidoscopy, double contrast barium enema, virtual colonoscopy, and optical colonoscopy [3,4] but none is completely specific, sensitive, or reliable [5]. It has also been demonstrated that inflammatory expression increases gradually as epithelial cells progress through dysplasia to neoplasia [6].…”

Section: Introductionmentioning

confidence: 99%

Fecal Calprotectin Levels in Patients with Colonic Polyposis

et al. 2007

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Context The usefulness of stool calprotectin determination in diagnosis of inflammatory disease of the colon has been reported; information about its usefulness for patients with polyposis are scarce, however. Objective To evaluate the significance of stool calprotectin concentrations for patients affected by colonic polyposis. Patients Sixty-three consecutive patients (35 males, 28 females, mean age 60.3 years, range 39-78 years) were enrolled: 26 patients (41.3%) with polyps, 17 patients (27.0%) with asymptomatic diverticular disease, and 20 subjects (31.7%) with normal endoscopic appearance of the colon. Results Stool calprotectin concentrations were 17.4 ± 24.5 lg g -1 for patients with colonic polyposis, significantly higher than concentrations for patients with diverticulosis (7.1 ± 5.7 lg g -1 ; P = 0.026) or for patients with normal appearance of the colon (calprotectin 6.0 ± 5.8 lg g -1 ; P = 0.003). For patients with a single polyp, stool calprotectin concentrations were similar to those for patients with multiple polyps. Calprotectin fecal concentrations for patients with sessile polyps and those with flat polyps were not significantly different. Calprotectin concentrations were not significantly related to the size of the polyps. Conclusion Our data show that colonic polyposis may cause an increase in stool calprotectin values and that these colonic lesions should be suspected when elevated stool calprotectin concentrations are found.

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Expression of Serum Amyloid A, in Normal, Dysplastic, and Neoplastic Human Colonic Mucosa: Implication for a Role in Colonic Tumorigenesis

Cited by 80 publications

References 43 publications

Serum amyloid A (SAA): a novel biomarker for uterine serous papillary cancer

Serum amyloid A (SAA): a novel biomarker for uterine serous papillary cancer

Gene Expression Patterns in the Human Breast after Pregnancy

Fecal Calprotectin Levels in Patients with Colonic Polyposis

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