Expression of serum miR‑27b and miR‑451 in patients with congenital heart disease associated pulmonary artery hypertension and risk factor analysis

Long, Leiwang; Xiao, Yunbin; Yin, Xiao-Cheng; Shun-li, Gao; Zhou, Leocadia; Liu, Hui

doi:10.3892/etm.2020.9042

Cited by 9 publications

(9 citation statements)

References 27 publications

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“…In a rat model of burn-induced acute lung damage, miR-451 expression is increased in lung endothelial cells, which promotes the increase of permeability of lung endothelial cells and inhibits the angiogenesis of lung endothelial cells, showing potential of burn treating [ 25 ]. Long et al found that decreased expression of miR-451 in serum of CHD-PAH patients, which is associated with BNP, ADMA, and disease severity [ 14 ]. Muscularization of hitherto nonmuscular arteries and remodeling of the pulmonary vasculature are two of PAH's primary hallmarks [ 26 ].…”

Section: Discussionmentioning

confidence: 99%

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Serum miR-204 and miR-451 Expression and Diagnostic Value in Patients with Pulmonary Artery Hypertension Triggered by Congenital Heart Disease

Qiao

Guan

et al. 2022

Computational and Mathematical Methods in Medicine

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Objective. To determine the level of expression and clinical importance of serum miR-204 and miR-451 in patients with pulmonary arterial hypertension caused by congenital heart disease (CHD-PAH). Methods. From July 2019 to January 2021, 114 infants with congenital heart disease (CHD) were hospitalized at Qingdao’s Fuwai Cardiovascular Hospital. They were grouped into categories: CHD (53 cases) and CHD-PAH (61 cases) based on whether they had pulmonary hypertension (PAH). In addition, 60 healthy children were selected as the control group. All children underwent routine biochemical examination, echocardiography, and pulmonary arterial pressure examination. By using an enzyme-linked immunosorbent assay (ELISA), the levels of brain natriuretic peptide (BNP) and asymmetric dimethylarginine (ADMA) in the blood were measured. Additionally, reverse transcription-polymerase chain reaction (RT-PCR) was used to determine the expression levels of miR-204 and miR-451 in peripheral blood. The correlation between miR-204, miR-451, BNP, ADMA, and mPAP was investigated using Pearson correlation analysis. Results. Consequently, the TC, BNP, and ADMA serum levels were considerably higher in the CHD and CHD-PAH groups than in the control group ( P < 0.05 ), whereas BNP and ADMA serum levels were significantly higher in the CHD-PAH group than in the CHD group ( P < 0.05 ). According to RT-PCR data, the expression levels of miR-204 and miR-451 in the peripheral blood of children in the CHD and CHD-PAH groups were significantly lower ( P < 0.05 ) when compared to the control group. The expression levels of miR-204 and miR-451 in the peripheral blood of children in the CHD-PAH group were substantially lower ( P < 0.05 ) than in the CHD group. Significantly, the findings of the ROC curve revealed that the area under the curve (AUC) of CHD-PAH diagnosed by miR-204 and miR-451 alone was 0.737 and 0.725, respectively, and the AUC of joint diagnosis was 0.840, which was greater than that of single diagnosis ( P < 0.05 ). Patients with CHD-PAH had lower levels of miR-204 and miR-451 in their blood, and this was found to be associated with BNP, ADMA, and mPAP, analyzed by Pearson correlation. Conclusions. Children with CHD-PAH can be diagnosed based on aberrant expressions of miR-204 and miR-451 in their peripheral blood serum. Moreover, CHD-PAH can be diagnosed if the combination of miR-204 and miR-451 is detected as a biomarker, which has a higher diagnostic value.

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Section: Discussionmentioning

confidence: 99%

“…MiR-451 is mainly expressed in cardiac myocytes and is upregulated in PHA patients and hypoxia-induced PAH rat models, and changes in its serum content affect the function of pulmonary artery smooth muscle cells [ 13 ]. Long et al found that the miR-451 expression was decreased in serum of patients with CHD-PAH [ 14 ].…”

Section: Introductionmentioning

confidence: 99%

Serum miR-204 and miR-451 Expression and Diagnostic Value in Patients with Pulmonary Artery Hypertension Triggered by Congenital Heart Disease

Qiao

Guan

et al. 2022

Computational and Mathematical Methods in Medicine

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“…Therefore, circulating miRNAs are a useful diagnostic and prognostic biomarker of PAH [ 49 , 50 ]. Previous studies have reported the association of circulating miRNAs, including miR-27b and miR-451 [ 51 ], miR-125a-5p [ 52 ], miR-509-3p [ 53 ], miR-206 [ 54 ], miR-483 [ 55 ], miR-211 [ 56 ], miR-143 [ 57 ], miR-596 [ 58 ], and miR-208-Mef2 [ 59 ] axis with the prognosis of PAH. The miR-29 family members have been reported as anti-fibrotic miRNAs [ 48 ].…”

Section: Discussionmentioning

confidence: 99%

miR-29a-3p/THBS2 Axis Regulates PAH-Induced Cardiac Fibrosis

Hsu

Liu

Kuo

et al. 2021

IJMS

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Pulmonary artery hypertension (PAH) pathology involves extracellular matrix (ECM) remodeling in cardiac tissues, thus promoting cardiac fibrosis progression. miR-29a-3p reportedly inhibits lung progression and liver fibrosis by regulating ECM protein expression; however, its role in PAH-induced fibrosis remains unclear. In this study, we aimed to investigate the role of miR-29a-3p in cardiac fibrosis progression in PAH and its influence on ECM protein thrombospondin-2 (THBS2) expression. The diagnostic and prognostic values of miR-29a-3p and THBS2 in PAH were evaluated. The expressions and effects of miR-29a-3p and THBS2 were assessed in cell culture, monocrotaline-induced PAH mouse model, and patients with PAH. The levels of circulating miR-29a-3p and THBS2 in patients and mice with PAH decreased and increased, respectively. miR-29a-3p directly targets THBS2 and regulates THBS2 expression via a direct anti-fibrotic effect on PAH-induced cardiac fibrosis. The circulating levels of miR-29a-3p and THBS2 were correlated with PAH diagnostic parameters, suggesting their independent prognostic value. miR-29a-3p targeted THBS2 expression via a direct anti-fibrotic effect on PAH-induced cardiac fibrosis, indicating miR-29a-3p acts as a messenger with promising therapeutic effects.

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“… 31 In congenital heart disease associated pulmonary artery hypertension, elevated serum miR-27b is considered to be of great significance in the diagnosis and evaluation of congenital heart disease associated pulmonary artery hypertension. 32 Due to the significant difference in expression of miR-27b between normal individuals and ACAS patients, its diagnostic value for ACAS was evaluated. A ROC curve based on serum miR-27b was constructed.…”

Section: Discussionmentioning

confidence: 99%

Circulating miR-27b as a Biomarker of the Development and Progression of Carotid Artery Stenosis

Lü

Long

et al. 2021

Clin Appl Thromb Hemost

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Objective A close relationship of microRNAs (miRNAs) with various human diseases has been widely reported, including cardiovascular disease. The current study attempted to examine the abnormal expression of miR-27b in asymptomatic carotid artery stenosis (ACAS), its diagnostic value and predictive value for the development of ACAS were also assessed. Methods Clinical serum samples were collected from both ACAS patients and healthy individuals, and levels of miR-27b in the clinical samples were detected using Real-time quantitative PCR. Cerebral ischemia events (CIEs) of patients during the 5-year follow-up were collected. The diagnostic and predictive values of serum miR-27b was assessed via plotting Receiver operating characteristic (ROC) and Kaplan-Meier curves. Multivariate cox regression analysis was performed for clinical independent index analysis. Results ACAS patients had higher levels of miR-27b than the healthy subjects. There were close association of serum miR-27b levels with total cholesterol (TC) level, absence of hypertension and degree of carotid stenosis. High levels of miR-27b could differentiate ACAS cases from healthy subjects, and predicted the high incidence of CIEs. MiR-27b could be used as an independent predictor of cerebrovascular events via multiple Cox regression analysis ( P = .031). Conclusion The high level of miR-27b can predict the occurrence of ACAS, and is closely related to the subsequent occurrence of CIEs. The present results provide evidence for circulating miR-27b as a diagnostic and prognostic marker in patients with ACAS.

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Expression of serum miR‑27b and miR‑451 in patients with congenital heart disease associated pulmonary artery hypertension and risk factor analysis

Cited by 9 publications

References 27 publications

Serum miR-204 and miR-451 Expression and Diagnostic Value in Patients with Pulmonary Artery Hypertension Triggered by Congenital Heart Disease

Serum miR-204 and miR-451 Expression and Diagnostic Value in Patients with Pulmonary Artery Hypertension Triggered by Congenital Heart Disease

miR-29a-3p/THBS2 Axis Regulates PAH-Induced Cardiac Fibrosis

Circulating miR-27b as a Biomarker of the Development and Progression of Carotid Artery Stenosis

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