Expression of Ski in the Granulosa Cells of Atretic Follicles in the Rat Ovary

Kim, Hyun; Yamanouchi, Keitaro; Nishihara, Masugi

doi:10.1262/jrd.18051

Cited by 11 publications

(14 citation statements)

References 28 publications

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“…Members of the TGF-b superfamily are critical regulators of follicle development, especially GDF-9 and BMP-15, which are expressed in an oocyte-specific manner from a very early stage and play a key role in promoting follicle growth beyond the primary stage [5]. TGF-b superfamily members are not the only factors to be expressed by oocytes, granulosa cells, and theca cells in a developmental stage related manner, functioning as intraovarian regulatory molecules vital to follicle recruitment, granulosa and theca cell proliferation/atresia, steroidogenesis, oocyte maturation, ovulation, and luteinization [6,7]. TGF-b superfamily receptors, ligands, and binding proteins are also expressed in a developmental stage manner in different ovarian compartments [2,[8][9][10].…”

Section: Introductionmentioning

confidence: 99%

Relationship between Sloan-Kettering virus expression and mouse follicular development

Tang

Zhang

2011

Endocrine

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Sloan-Kettering virus gene product (Ski) is an unique nuclear pro-oncoprotein and belongs to the ski/sno proto-oncogene family. Ski plays multiple roles in a variety of cell types, it can induce both oncogenic transformation and terminal muscle differentiation when expressed at high levels. Ski/SnoN are important transcription regulators of the transforming growth factor-β (TGF-β) superfamily and function mainly through heterodimers. Since TGF-β superfamily are key regulators of follicle development and it has been previously shown that SnoN is also vital to follicle development, this research was conducted to clarify the relationship between Ski expression and mouse follicular development, in ovaries of neonatal and gonadotropin-induced immature mice by immunohistochemical and real-time PCR techniques. In postnatal mice, positive staining for Ski was highly detected in oocyte nuclei at postnatal day 1. With follicular development, the localization moved gradually from oocyte nuclei to perinuclear space and the total levels decreased. During the estrous cycle, Ski expression was apparent at proestrus and estrus, faint at metestrus, highest at diestrus. After injection of gonadotropin, Ski was found in perinuclear space and weak in oocyte nuclei. Following the initiation of luteinization, the expression of Ski was found in corpus luteum. Real-time PCR results also showed that Ski mRNA expression was opposite to ovulation-related genes during the cumulus expansion, with the development of the follicles, its expression level decreased. Ski is expressed in a specific manner during follicle development, ovulation and luteinization. So Ski might play essential roles in these processes especially during early follicular development.

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Section: Introductionmentioning

confidence: 99%

Relationship between Sloan-Kettering virus expression and mouse follicular development

Tang

Zhang

2011

Endocrine

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“…We previously reported that the Ski protein is expressed in granulosa cells of atretic follicles [20]. In order to determine if the Ski-positive cells that appeared after hCG treatment were the cells that were undergoing apoptosis, we analyzed apoptosis of these cells using a TUNEL assay.…”

Section: Resultsmentioning

confidence: 99%

“…Immunohistochemical analyses of Ski were performed as described previously [20]. In brief, frozen tissue sections (5 μm thick) prepared from the OCT-embedded rat ovary using a cryostat (Microm HM550, Carl Zeiss Japan, Tokyo, Japan) were mounted on glass slides, fixed in 4% paraformaldehyde (PFA) in phosphate bufferedsaline (PBS) for 20 min and then incubated in 0.1% Triton X-100 in PBS for 15 min.…”

Section: Immunohistochemistry and Tunel Stainingmentioning

confidence: 99%

“…In addition to its transforming activity, c-ski is known to induce myogenic differentiation of quail embryonic cells [12]. Thus, c-ski has been suggested to have dual roles in the cell, regulating both transformation (proliferation) and differentiation of cells.We previously showed that Ski is present in granulosa cells of atretic follicles in the rat ovary, whereas it is absent from granulosa cells of preovulatory follicles [20]. Since the TGF-β family plays important roles during luteinization, it is highly likely that Ski is involved in this process.…”

mentioning

confidence: 99%

“…We previously showed that Ski is present in granulosa cells of atretic follicles in the rat ovary, whereas it is absent from granulosa cells of preovulatory follicles [20]. Since the TGF-β family plays important roles during luteinization, it is highly likely that Ski is involved in this process.…”

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confidence: 99%

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Induction of Ski Protein Expression upon Luteinization in Rat Granulosa Cells without a Change in its mRNA Expression

Kim

Yamanouchi

Matsuwaki

et al. 2012

Journal of Reproduction and Development

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Abstract. The Ski protein is implicated in the proliferation/differentiation of a variety of cells. We previously reported that the Ski protein is present in granulosa cells of atretic follicles, but not in preovulatory follicles, suggesting that Ski has a role in apoptosis of granulosa cells. However, granulosa cells cannot only undergo apoptosis but can alternatively differentiate into luteal cells. It is unknown whether Ski is expressed and has a role in granulosa cells undergoing luteinization. Thus, the aim of the present study was to determine the localization of the Ski protein in the rat ovary during luteinization to examine if Ski might play a role in this process. In order to examine the Ski protein expression during the progression of luteinization, follicular growth was induced in immature female rats by administration of equine chorionic gonadotropin, and luteinization was induced by human chorionic gonadotropin treatment to mimic the luteinizing hormone (LH) surge. While no Ski-positive granulosa cells were present in the preovulatory follicle, Ski protein expression was induced in response to the LH surge and was maintained after formation of the corpus luteum (CL). Although the Ski protein is absent from the granulosa cells of the preovulatory follicle, its mRNA (c-ski) was expressed, and the level of c-ski mRNA was unchanged even after the LH surge. The combined results demonstrated that Ski protein expression is induced in granulosa cells upon luteinization, and suggested that its expression is regulated posttranscriptionally. O varian folliculogenesis and luteinization in mammals is a complex series of events that is regulated by endocrine and paracrine/autocrine factors. The role of the endocrine factors, gonadotropins, follicle stimulating hormone (FSH) and luteinizing hormone (LH), in regulating folliculogenesis and luteinization is well established. Gonadotropin is an important survival factor for developing follicles that allows their escape from atresia and stimulates early antral follicles to complete their final differentiation and reach the preovulatory follicle stage. A preovulatory follicle responds to the LH surge by embarking on a terminal differentiation pathway termed "luteinization" that transforms granulosa and theca cells of a preovulatory follicle into luteal cells that form the corpus luteum (CL). Luteinizing follicular cells undergo specific morphological and biochemical changes, as well as endocrinological alterations, in their transition to luteal cells [1,2]. Many genes that are highly expressed in growing preovulatory follicles are turned off by the LH surge, whereas the expression of numerous genes that are involved in ovulation and luteinization increases dramatically during the periovulatory period [3,4].The effects of gonadotropins are mediated or regulated by a number of paracrine/autocrine growth factors [5,6]. For example, members of the transforming growth factor-β (TGF-β) family are expressed by oocytes, granulosa cells and theca cells in a developmental-stage...

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An investigation of the effects of chronic zonisamide, sultiam, lacosamide, clobazam, and rufinamide anti‐seizure medications on foliculogenesis in ovarian tissue in prepubertal non‐epileptic rats

Kart

Gürgen

Eyüboğlu

et al. 2022

Intl J of Devlp Neuroscience

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Expression of Ski in the Granulosa Cells of Atretic Follicles in the Rat Ovary

Cited by 11 publications

References 28 publications

Relationship between Sloan-Kettering virus expression and mouse follicular development

Relationship between Sloan-Kettering virus expression and mouse follicular development

Induction of Ski Protein Expression upon Luteinization in Rat Granulosa Cells without a Change in its mRNA Expression

An investigation of the effects of chronic zonisamide, sultiam, lacosamide, clobazam, and rufinamide anti‐seizure medications on foliculogenesis in ovarian tissue in prepubertal non‐epileptic rats

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