Expression of Skp2 and p27kip1 proteins in hypopharyngeal squamous cell carcinoma and its clinical significance

Abstract

Abstract. The aim of the present study was to determine the effect of S-phase kinase-associated protein 2 (Skp2) and cyclin-dependent kinase inhibitor p27 kinase-interacting protein 1 (p27 kip1 ) protein expression on the occurrence and development of hypopharyngeal squamous cell carcinoma. The protein expression levels of Skp2 and p27 kip1 were detected in 42 hypopharyngeal squamous cell carcinoma and 15 normal hypopharyngeal mucous membrane specimens using the an immunohistochemical PV-9000 two-step method. The expression levels of Skp2 protein were significantly different in hypopharyngeal squamous cell carcinomas and normal hypopharyngeal mucous membranes (61.90 vs. 26.67%; P<0.05). By contrast, the protein expression levels of Skp2 were significantly positively correlated with tumor T stage (r s =0.329, P<0.05) and cervical lymph node metastasis (r s =0.402, P<0.05). Furthermore, the expression levels of p27 kip1 protein were significantly different in hypopharyngeal squamous cell carcinomas and normal hypopharyngeal mucous membranes (11.9 vs. 53.33%; P<0.05), while p27 kip1 protein expression was significantly negatively correlated with tumor T-stage (r s =-0.351, P<0.05) and cervical lymph node metastasis (r s =-0.371, P<0.05). Notably, a significant negative correlation was observed between the expression levels of Skp2 and p27 kip1 proteins in hypopharyngeal squamous cell carcinoma (P<0.05). In addition, abnormal expression levels of Skp2 and p27 kip1 proteins were observed in hypopharyngeal squamous cell carcinoma tissues. Thus, Skp2 and p27 kip1 proteins may be involved in the development of hypopharyngeal squamous cell carcinoma. The current study proposed that combined detection of Skp2 and p27 kip1 may be useful for assessing the characteristics and prognosis of hypopharyngeal squamous cell carcinoma.

Antisense oligonucleotide p45Skp-2 suppresses migratory chemotactic and metastasis of oral malignant Burkitt’s lymphoma cell through down-regulation of MTA-1 and induction of E-cadherin mechanism

“…We also have research limitations in the form of research materials that must be obtained abroad and take a long time to be available, research instruments that were limited and queued up, and complicated research permit correspondence. p27 Kip1 level have been observed in lymphomas 8 , oral squamous cell carcinomas 3,16 , lung cancer 2 and gastric carcinoma. 9 In the present study, the phosphorotioate oligonucleotide AS and S strategy was delivered to investigate the activity of Skp-2 on migratory chemotactic and metastasis suppresion in an oral malignant Burkitt's lymphoma cell line (Raji cells) through the down-regulation of MTA-1 and induction of E-cadherin expression.…”

Role of P27kip1 Protein, P45skp2Coactivator, and P38jab1 Coactivator In Preventing Rhabdomyosarcoma (RMS) in Oral Cavity of Children

“…As well as Jab1 and p27 kip1 started to reveal that the association became known widely in hepatocellular carcinoma, laryngeal carcinoma, and nerves. 19 Other investigators suggest that Skp2 may degrade p27 kip1 protein through the ubiquitinproteasome pathway, thus, participating in the occurrence and development of hypopharyngeal squamous cell carcinoma. Furthermore, the abnormal protein expressions Skp2 and p27 kip1 appear to be associated with the poor prognosis of hypopharyngeal squamous cell carcinoma.…”

The Impact of the Ubiquitin System in the Pathogenesis of Squamous Cell Carcinomas

“…During squamous carcinogenesis there is a marked expansion of the SKP2 positive cells and a corresponding reduction in p27-positive cells [64]. Notably, the inverse correlation between p27 and SKP2 proteins levels represents a negative prognostic factor in SCCs [65][66][67].…”