Expression of small heat shock proteins HspB2, HspB8, Hsp20 and cvHsp in different tissues of the perinatal developing pig

Verschuure, Pauline; Tatard, Caroline; Boelens, Wilbert C.; Grongnet, Jean‐François; David, Jean‐Claude

doi:10.1078/0171-9335-00337

Cited by 79 publications

(87 citation statements)

References 30 publications

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“…Related to ␣-crystallin, a new series of sHSPs has recently been described (Horwitz 2003;Verschuure et al 2003). Two of them (HSPB2 and HSPB20) are expressed in different tissues, particularly in muscle and the heart (Verschuure et al 2003). To our knowledge, the expression of these proteins has never been studied in the heart of animals subjected to conditions of hypoxia.…”

Section: Introductionmentioning

confidence: 97%

“…␣B-crystallin is present in different tissues including skeletal muscle and the heart (Tallot et al 2003). Related to ␣-crystallin, a new series of sHSPs has recently been described (Horwitz 2003;Verschuure et al 2003). Two of them (HSPB2 and HSPB20) are expressed in different tissues, particularly in muscle and the heart (Verschuure et al 2003).…”

Section: Introductionmentioning

confidence: 99%

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Effects of hypoxia on stress proteins in the piglet heart at birth

Louapre

Grongnet²,

Tanguay

et al. 2005

Cell Stress Chaper

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Hypoxia at birth represents a very stressful event that can result in severe lifelong consequences in different tissues, including those of the heart. Heat shock and other associated stress proteins are involved in cellular protection, but their roles are not clearly defined at the time of birth. Newborn piglets were subjected to 5% oxygen and 95% nitrogen for either 1 or 4 hours. They were allowed to recover over periods of 1 to 68 hours. The relative levels of ␣B-crystallin, HspB8, Hsp20, Hsp27, Hsp60, and Hsp70 as well as nitric oxide synthases (NOS) (endothelial NOS, inducible NOS, neuronal NOS) were examined by Western blot analysis. Surprisingly, ␣B-crystallin expression was drastically increased in animals submitted to hypoxia. The hypoxia-associated factor HIFI␣ was also strongly and rapidly overexpressed. Heme oxygenase 1 was also increased. To a lesser extent, neuronal NOS was also increased in the left ventricle of animals submitted to hypoxia. This work clearly shows that the Hsp chaperone ␣B-crystallin is strongly overexpressed in the left ventricle of animals submitted to hypoxia. This observation dissociates the response to low oxygenation of ␣B-crystallin and other stress-associated proteins including Hsp27, and it indicates that heme oxygenase is not alone among HSPs in its oxygen-related gene expression.

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Section: Introductionmentioning

confidence: 97%

Section: Introductionmentioning

confidence: 99%

Effects of hypoxia on stress proteins in the piglet heart at birth

Louapre

Grongnet²,

Tanguay

et al. 2005

Cell Stress Chaper

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“…Hsp20 has received special attention because of its localization in muscle and heart [31,32] . Moreover, it has been demonstrated that Hsp20 is also localized in different brain areas and its expression changes in response to hypoxic stress [33] .…”

Section: Discussionmentioning

confidence: 99%

Modulation of heat shock protein response in SH-SY5Y by mobile phone microwaves

Calabrò

Condello²,

Currò³

et al. 2012

WJBC

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“…Verschuure et al (2003) detected by Western blot the constitutive expression of this protein and other Hspbs in samples of the cortex, cerebellum, hippocampus, and striatum of the perinatal pig. Similarly, Kirbach and Golenhofen (2010) described the constitutive expression of Hspb8 mRNA in the same tissues from the rat brain as detected by quantitative real-time RT-PCR, reporting as well its upregulation by heat-shock in cultured hippocampal neurons.…”

Section: Introductionmentioning

confidence: 97%

Developmental Expression Pattern of Hspb8 mRNA in the Mouse Brain: Analysis Through Online Databases

García-Lax

Tomás‐Roca

Marı́n

2011

The Anatomical Record

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Hspb8 is a member of the Hspb family of chaperone-like proteins. It is involved in several neural disorders such as Alzheimer's disease, amyotrophic lateral sclerosis, hereditary distal motor neuropathy, and CharcotMarie-Tooth's disease. In this work, we aimed to characterize its expression pattern in the mouse brain, by using the information available at online databases of high-throughput in situ hybridization. Therefore, we downloaded and analyzed the image series from these databases showing Hspb8 mRNA expression from embryonic to adult and aging stages. In early gestational embryos, Hspb8 was expressed in the hippocampal anlagen and in the ventricular layer of rhombomere 4. At perinatal stages, there appeared transitory expression in the dentate gyrus and the cerebellar cortex. From perinatal to aging stages, the neurons of the mesencephalic trigeminal nucleus and cranial motor nuclei displayed stable and strong Hspb8 expression. Additionally, along these stages there was moderate and relatively homogenous expression in the anterodorsal thalamic, lateral mammillary, arcuate hypothalamic and medial habenular nuclei, and in the locus coeruleus. In its turn, the basal ganglia, cerebellar inner granular layer and diverse sensory and reticular formation nuclei of the

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Expression of small heat shock proteins HspB2, HspB8, Hsp20 and cvHsp in different tissues of the perinatal developing pig

Cited by 79 publications

References 30 publications

Effects of hypoxia on stress proteins in the piglet heart at birth

Effects of hypoxia on stress proteins in the piglet heart at birth

Modulation of heat shock protein response in SH-SY5Y by mobile phone microwaves

Developmental Expression Pattern of Hspb8 mRNA in the Mouse Brain: Analysis Through Online Databases

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