Expression of soybean-embryo lipoxygenase 2 in transgenic tobacco tissue

Deng, Wenlian; Grayburn, W. Scott; Hamilton-Kemp, Thomas R.; Collins, G. B.; Hildebrand, David F.

doi:10.1007/bf00201939

Cited by 11 publications

(5 citation statements)

References 31 publications

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“…The availability of sufficient amounts of substrate seems to play a crucial role in the activation of the LOX pathway as tobacco plants overexpressing different LOX genes show no or only moderately elevated levels of LOX-derived metabolites (50,51). Because linoleic acid-derived oxylipins were the major derivatives detected in elicitor-treated potato cells, the linoleic acid pool was analyzed in two different ways: (i) by studying mRNA accumulation of ⌬ 12 -desaturases, which catalyze the formation of linoleic acid from oleic acid at the sn2 position of PC within the endoplasmic reticulum, and (ii) by measuring activity of PLA 2 , the major enzyme responsible for release of these PUFAs into the cytosol.…”

mentioning

confidence: 99%

Oxylipin Profiling Reveals the Preferential Stimulation of the 9-Lipoxygenase Pathway in Elicitor-treated Potato Cells

Göbel¹,

Feußner

Schmidt

et al. 2001

Journal of Biological Chemistry

161

105

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Lipoxygenases are key enzymes in the synthesis of oxylipins and play an important role in the response of plants to wounding and pathogen attack. In cultured potato cells treated with elicitor from Phytophthora infestans, the causal agent of late blight disease, transcripts encoding a linoleate 9-lipoxygenase and a linoleate 13-lipoxygenase accumulate. However, lipoxygenase activity assays and oxylipin profiling revealed only increased 9-lipoxygenase activity and formation of products derived therefrom, such as 9-hydroxy octadecadienoic acid and colneleic acid. Furthermore, the 9-lipoxygenase products 9(S),10(S),11(R)-trihydroxy-12(Z)-octadecenoic and 9(S),10(S),11(R)-trihydroxy-12(Z),15(Z)-octadecadienoic acid were identified as novel, elicitor-inducible oxylipins in potato, suggesting a role of these compounds in the defense response against pathogen attack. Neither 13-lipoxygenase activity nor 13-lipoxygenase products were detected in higher amounts in potato cells after elicitation. Thus, formation of products by the 9-lipoxygenase pathway, including the enzymes hydroperoxide reductase, divinyl ether synthase, and epoxy alcohol synthase, is preferentially stimulated in cultured potato cells in response to treatment with P. infestans elicitor. Moreover, elicitor-induced accumulation of desaturase transcripts and increased phospholipase A 2 activity after elicitor treatment suggest that substrates for the lipoxygenase pathway might be provided by de novo synthesis and subsequent release from lipids of the endomembrane system.

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mentioning

confidence: 99%

Oxylipin Profiling Reveals the Preferential Stimulation of the 9-Lipoxygenase Pathway in Elicitor-treated Potato Cells

Göbel¹,

Feußner

Schmidt

et al. 2001

Journal of Biological Chemistry

161

105

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“…54,224 Besides the discussed expression hosts (Tables 2 and 3), LOXs were also expressed in transgenic plants. 225,226…”

Section: Recombinant Loxsmentioning

confidence: 99%

Recombinant Lipoxygenases and Hydroperoxide Lyases for the Synthesis of Green Leaf Volatiles

Stolterfoht

Rinnofner

Winkler

et al. 2019

J. Agric. Food Chem.

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Green leaf volatiles (GLVs) are mainly C 6 -and in rare cases also C 9 -aldehydes, -alcohols, and -esters, which are released by plants in response to biotic or abiotic stresses. These compounds are named for their characteristic smell reminiscent of freshly mowed grass. This review focuses on GLVs and the two major pathway enzymes responsible for their formation: lipoxygenases (LOXs) and fatty acid hydroperoxide lyases (HPLs). LOXs catalyze the peroxidation of unsaturated fatty acids, such as linoleic and α-linolenic acids. Hydroperoxy fatty acids are further converted by HPLs into aldehydes and oxo-acids. In many industrial applications, plant extracts have been used as LOX and HPL sources. However, these processes are limited by low enzyme concentration, stability, and specificity. Alternatively, recombinant enzymes can be used as biocatalysts for GLV synthesis. The increasing number of well-characterized enzymes efficiently expressed by microbial hosts will foster the development of innovative biocatalytic processes for GLV production.

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“…Hexenal, hexenol reduced to 1.5% of wild-type [329] Nicotiana tabacum LOX2 of soybean was overexpressed in tobacco under CaMV35S promoter. There was a 50-529% increase in C6 volatile aldehyde production [330] Solanum tuberosum The potato plastidic LOX H1 was silenced by antisense methodology. Volatile C6 aldehydes were reduced.…”

Section: Arabidopsis Thalianamentioning

confidence: 99%

Biotechnology for the production of essential oils, flavours and volatile isolates. A review.

Gounaris

2010

Flavour & Fragrance J

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Various applications of biotechnological methods for the production of volatile compounds useful to the food and pharmaceutical industries are discussed. The yields obtained from intact or genetically modified plants are compared to those achieved by microbial methods. Plant yields are too low for the products to compete commercially to those synthesized chemically. Still lower yields are obtained with in vitro-cultured plant tissues. Trangenic plants with altered methylerythritol path gave 50% more essential oil in the best case. The 100-fold increases in shikimate-derived volatiles, obtained with overexpressed alcohol dehydrogenase and five-fold more C6 volatle aldehydes and 2-phenylethanol, were produced with overexpressed lipoxygenase and 2-phenylethanol dehydrogenase, respectively. However, the most spectacular yields were observed with biotransformations catalysed by microorganisms. Kluyveromyces marxianus, produces over 26 g/l 2-phenylethanol from phenyalanine, whereas Candida sorbophila, Mucor circillenoides or Yarrowia lipolytica can produce 5-40 g/l g-decalactone from ricinoleic acid. Vanillin production from ferulic acid is in the range 12-60 g/l with Amycolatopsis and Streptomyces species. Vanillin can be produced at 5 g/l by Escherichia coli and amorphadiene yields of 37 g/l have been observed with Saccharomyces cerevisiae, both with the genetically overexpressed methyl-erythritol path. Genetically engineered b-oxidation genes result in yields of 10 g/l g-decalactone byYarrowia lipolytica and up to 80 g/l dicaboxylic acids by various yeasts. These results far exceed the theoretical limit of about 1 g/l required for consideration of a procedure as a commercially interesting process, alternative to chemical sythesis.

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Expression of soybean-embryo lipoxygenase 2 in transgenic tobacco tissue

Cited by 11 publications

References 31 publications

Oxylipin Profiling Reveals the Preferential Stimulation of the 9-Lipoxygenase Pathway in Elicitor-treated Potato Cells

Oxylipin Profiling Reveals the Preferential Stimulation of the 9-Lipoxygenase Pathway in Elicitor-treated Potato Cells

Recombinant Lipoxygenases and Hydroperoxide Lyases for the Synthesis of Green Leaf Volatiles

Biotechnology for the production of essential oils, flavours and volatile isolates. A review.

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