Expression of specific (1→3)-β-glucanase genes in leaves of near-isogenic resistant and susceptible barley lines infected with the leaf scald fungus (Rhynchosporium secalis)

Roulin, Samuel; Xu, Peng; Brown, A. H. D.

doi:10.1006/pmpp.1997.0084

Cited by 32 publications

(13 citation statements)

References 38 publications

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“…More detailed studies have confirmed the expression of the isoenzyme GII gene in this system (Jutidamrongphan et al 1991), although there is also evidence for enhanced expression of the Abg2 (1^3)-/?-glucanase gene during infection (Malehorn et al 1993). Similarly, infection of barley leaves with the scald fungus, Rhynchosporium secalis, leads to the induction of the (l-^3)-^-glucanase isoenzyme GII gene (Roulin et al 1997). The detection of (1^3)-j9-glucanase isoenzyme GII mRNA in uninduced cells (Fig.…”

Section: Induction Of Specific (1->3)-)9-glucanase Isoenzymesmentioning

confidence: 87%

N-acetylchitooligosaccharides elicit expression of a single (13)-beta-glucanase gene in suspension-cultured cells from barley (Hordeum vulgare)

Kaku¹,

Shibuya²,

Xu³

et al. 1997

Physiol Plant

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Section: Induction Of Specific (1->3)-)9-glucanase Isoenzymesmentioning

confidence: 87%

N-acetylchitooligosaccharides elicit expression of a single (13)-beta-glucanase gene in suspension-cultured cells from barley (Hordeum vulgare)

Kaku¹,

Shibuya²,

Xu³

et al. 1997

Physiol Plant

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“…4). Basic b-1,3-glucanases have also been reported to be induced in response to fungal infection in pepper (Kim & Hwang, 1994), barley (Ignatius et al, 1994;Roulin et al, 1997), tomato (Van Kan et al, 1992 and potato (Beerhues & Kombrink, 1994).…”

Section: Discussionmentioning

confidence: 99%

Induction of different pathogenesis‐related cDNAs in grapevine infected with powdery mildew and treated with ethephon

1999

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Uncinula necator, the causal agent of grapevine powdery mildew, was found to elevate the activity of the pathogenesis‐related (PR) proteins, chitinase and β‐1,3‐glucanase in leaves and berries of a number of susceptible grapevine (Vitis vinifera) cultivars. The increase in hydrolytic activity was directly related to the severity of powdery mildew infection on both leaves and berries but no systemic induction was observed in uninfected tissues. A number of cDNA clones encoding various PR proteins, including chitinases (PR‐2), β‐1,3‐glucanases (PR‐3) and thaumatin‐like (TL) proteins (PR‐5), were isolated and their expression in response to powdery mildew infection examined. PR genes encoding extracellular proteins were most strongly induced in infected leaves, including an acidic class III chitinase (VvChi3), a basic class I glucanase (VvGlub) and a thaumatin‐like protein (VvTl2). A basic class I chitinase (VvChi1b) was also detected, but only in severely infected leaves. A similar pattern of PR gene induction was observed in mildew‐infected preveraison berries, with the exception of VvChi1b, which was not detected. Chitinase and β‐1,3‐glucanase enzyme activities were also found to be highly induced in leaves and preveraison berries by ethephon treatment. The pattern of chitinase gene induction by ethephon, however, was markedly different for mildew infection, with no apparent induction of VvChi3 but a marked increase in the transcript level of an acidic class IV chitinase, VvChi4. These results demonstrate that the leaves and preveraison berries of grapevine respond to environmental and stress‐related stimuli by the expression of specific PR genes but that the induction of these genes during pathogen invasion does not afford complete protection of these tissues against Uncinula necator. The following abbreviations have been used: Tris: 2‐amino‐2‐(hydroxymethyl)‐1,3‐propanediol; EDTA: ethylenediaminetetra‐acetic acid; PVPP: polyvinylpolypyrrolidone. The GenBank accession numbers for VvChi1b and VvGlub are AF053341 and AF053750, respectively.

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“…According to Joosten and De Wit (1989), the 35‐kDa β ‐1,3‐glucanase, as well as the total β ‐1,3‐glucanase activity in apoplastic fluids of Cladosporium fulvum ‐infected tomato leaves, increases 4 days earlier in incompatible interactions than in compatible ones. In barley, induction of β ‐1,3‐glucanase activity occurs 1 day earlier, and to higher levels, in the resistant line BC‐200 infected with Rhynchosporium secalis than in a susceptible line (Roulin et al 1997). These responses are consistent with the notion that resistant plants respond more quickly to pathogen invasion than susceptible plants and that β ‐1,3‐glucanase contributes to resistance against different fungi.…”

Section: Discussionmentioning

confidence: 99%

Intercellular proteins and β‐1,3‐glucanase activity associated with leaf rust resistance in wheat

Anguelova

Westhuizen

Pretorius

1999

Physiologia Plantarum

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To investigate biochemical aspects of resistance conferred by the Lr35 gene for adult‐plant resistance in wheat (Triticum aestivum L.) to leaf rust, pathogen development was related to intercellular protein composition and β‐1,3‐glucanase (EC 3.2.1.39) activities at three growth stages in infected and uninfected resistant (RL6082 [Thatcher/Lr35]) and susceptible (Thatcher) plants. Leaf rust symptoms produced by pathotype UVPrt9 of Puccinia recondita f. sp. tritici showed that resistance conferred by Lr35 was most effective at the flag leaf stage. Furthermore, fluorescence microscopy indicated that resistance was strongly associated with hypersensitive cell death of invaded tissue. According to polypeptide profiles, intercellular proteins with molecular masses of 35, 33, 31 and 26 kDa were constitutively present at higher levels in resistant than in susceptible plants at the flag leaf stage. Four intercellular proteins (35, 33, 32 and 31 kDa) serologically related to β‐1,3‐glucanase were present in resistant and susceptible genotypes during all stages of plant growth. Resistance was associated with high constitutive levels of β‐1,3‐glucanase activity. Susceptibility on the other hand was associated with low constitutive levels of β‐1,3‐glucanase, while high levels were induced by infection during more advanced stages of colonization. Our results suggest that β‐1,3‐glucanase is involved in the defense response controlled by the Lr35 gene.

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Expression of specific (1→3)-β-glucanase genes in leaves of near-isogenic resistant and susceptible barley lines infected with the leaf scald fungus (Rhynchosporium secalis)

Cited by 32 publications

References 38 publications

N-acetylchitooligosaccharides elicit expression of a single (13)-beta-glucanase gene in suspension-cultured cells from barley (Hordeum vulgare)

N-acetylchitooligosaccharides elicit expression of a single (13)-beta-glucanase gene in suspension-cultured cells from barley (Hordeum vulgare)

Induction of different pathogenesis‐related cDNAs in grapevine infected with powdery mildew and treated with ethephon

Intercellular proteins and β‐1,3‐glucanase activity associated with leaf rust resistance in wheat

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