Expression of SPRR3 is associated with tumor cell proliferation and invasion in glioblastoma multiforme

Liu, Qingyang; Zhang, Chuanbao; Ma, Guofo; Zhang, Quangeng

doi:10.3892/ol.2013.1736

Cited by 21 publications

(17 citation statements)

References 31 publications

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“…SPRR-v1 is highly expressed in the esophageal mucosa of healthy individuals, whereas SPRR3-v2 expression is higher in the adjacent mucosal tissues in patients with esophageal squamous cell carcinoma [21]. High SPRR3 expression also has been reported in colorectal cancer, breast cancer, and glioblastoma multiforme (the most common primary brain tumor in adults); thus, this protein is a reported candidate biomarker for cancer diagnosis [22][23][24].…”

Section: Discussionmentioning

confidence: 93%

Identification and evaluation of potential forensic marker proteins in vaginal fluid by liquid chromatography/mass spectrometry

2015

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Vaginal fluid is one of the most common body fluids found at crime scenes. Discriminating vaginal fluid from other body fluids is important in forensic science; however, few potential protein markers have been reported to date. Proteomic methods for identifying protein markers have gained attention, although few reports have applied this technology to forensic protein markers. Therefore, to identify characteristic vaginal proteins, we examined various body fluids (nasal secretions, saliva, urine, semen, vaginal fluids, and sweat) using liquid chromatography/electrospray ionization time-of-flight mass spectrometry and peptide mass fingerprinting. We identified three components (average molecular mass values 17,237 ± 2, 18,063 ± 2, and 15,075 ± 1) detectable only in vaginal samples: two human small proline-rich protein 3 (SPRR3) isoforms and a human fatty acid-binding protein 5 (FABP5) with an acetylated (+42) N-terminal region lacking the initiator methionine residue (-131). Using ELISA, these yielded markedly high average values in vaginal fluids. The mass spectra of these proteins were not detected in infant saliva but were detected in the vaginal fluid throughout the menstrual cycle. The results of forensic analysis (detection limit, mixed body fluid samples, casework samples, and blind samples) suggest that these proteins are potential forensic markers. In conclusion, high SPRR3 and FABP5 expression levels, which may be used as potential markers for vaginal fluid identification in forensic science, were detected in vaginal fluids from healthy adults.

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Section: Discussionmentioning

confidence: 93%

Identification and evaluation of potential forensic marker proteins in vaginal fluid by liquid chromatography/mass spectrometry

2015

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“…Small proline-rich protein 3 (SPPR3) was detected at higher levels in the urine of both primary and recurrent urothelial bladder cancer patients, in comparison with controls. SPRR3 protein has not been characterized in the context of bladder cancer yet, however, upregulation of SPRR3 protein levels promotes colorectal tumorigenesis (37) and is associated with tumor cell proliferation and invasion in glioblastoma (38). In addition, 14-3-3 sigma protein is frequently downregulated in a variety of human cancers including invasive urothelial bladder cancer tumors, particularly in lesions undergoing epithelial to mesenchymal transition (39) and this downregulation is attributed to increased methylation of its promoter (40).…”

Section: Discussionmentioning

confidence: 99%

Development and Validation of Urine-based Peptide Biomarker Panels for Detecting Bladder Cancer in a Multi-center Study

Frantzi

Kessel

Zwarthoff

et al. 2016

Clinical Cancer Research

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Purpose: Urothelial bladder cancer presents high recurrence rates, mandating continuous monitoring via invasive cystoscopy. The development of noninvasive tests for disease diagnosis and surveillance remains an unmet clinical need. In this study, validation of two urine-based biomarker panels for detecting primary and recurrent urothelial bladder cancer was conducted.Experimental Design: Two studies (total n ¼ 1,357) were performed for detecting primary (n ¼ 721) and relapsed urothelial bladder cancer (n ¼ 636). Cystoscopy was applied for detecting urothelial bladder cancer, while patients negative for recurrence had follow-up for at least one year to exclude presence of an undetected tumor at the time of sampling. Capillary electrophoresis coupled to mass spectrometry (CE-MS) was employed for the identification of urinary peptide biomarkers. The candidate urine-based peptide biomarker panels were derived from nested cross-sectional studies in primary (n ¼ 451) and recurrent (n ¼ 425) urothelial bladder cancer.Results: Two biomarker panels were developed on the basis of 116 and 106 peptide biomarkers using support vector machine algorithms. Validation of the urine-based biomarker panels in independent validation sets, resulted in AUC values of 0.87 and 0.75 for detecting primary (n ¼ 270) and recurrent urothelial bladder cancer (n ¼ 211), respectively. At the optimal threshold, the classifier for detecting primary urothelial bladder cancer exhibited 91% sensitivity and 68% specificity, while the classifier for recurrence demonstrated 87% sensitivity and 51% specificity. Particularly for patients undergoing surveillance, improved performance was achieved when combining the urine-based panel with cytology (AUC ¼ 0.87).Conclusions: The developed urine-based peptide biomarker panel for detecting primary urothelial bladder cancer exhibits good performance. Combination of the urine-based panel and cytology resulted in improved performance for detecting disease recurrence.

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“…5 High expression of SPRR3 is associated with cancer carcinogenesis in glioblastoma multiform, colorectal and breast cancer. [6][7][8] SPRR3 has also been reported to interact with c-Jun and Fra1 in human bronchial epithelial cells. 9 Besides, SPRR3 is found to play roles in vascular smooth muscle cells (VSMCs) adaption to local biomechanical stress.…”

Section: Introductionmentioning

confidence: 99%

<p>Dysregulation of SPRR3/miR-876-3p Axis Contributes to Tumorigenesis in Non-Small-Cell Lung Cancer</p>

Qin¹,

Wang²,

Hu³

et al. 2020

OTT

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These authors contributed equally to this workBackground: SPRR3, also known as esophagin, has been shown to be involved in the initiation and progression of numerous types of tumor. However, the biological function of SPRR3 that contributes to non-small-cell lung cancer (NSCLC) growth and migration is largely unknown. Methods: The expression of SPRR3 and its association with EZH2 and miR-876-3p in NSCLC cells were determined by real-time PCR. Protein levels were measured by immunohistochemistry (IHC) and Western blot. Cell functions were studied by CCK-8, transwell assay, flow cytometry and dual-luciferase reporter assay. The effect of SPRR3 on tumor growth in vivo was evaluated in patient-derived xenograft (PDX) models. Results: SPRR3 was up-regulated in most NSCLC cell lines and clinical tissues. Also, the correlation between SPRR3 expression and clinical features was significant. Functional studies confirmed that SPRR3 modulates cell proliferation, invasion and cell apoptosis in NSCLC via regulating EZH2, which is a well-known oncogene in NSCLC. Furthermore, SPRR3 was found to be a direct target of miR-876-3p that also plays a suppressor role in NSCLC. Conclusion: These findings indicated that miR-876-3p/SPRR3/EZH2 signaling cascade exerts important roles in the regulation of NSCLC, suggesting that this pathway can serve as a potential therapeutic target in NSCLC.

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Expression of SPRR3 is associated with tumor cell proliferation and invasion in glioblastoma multiforme

Cited by 21 publications

References 31 publications

Identification and evaluation of potential forensic marker proteins in vaginal fluid by liquid chromatography/mass spectrometry

Identification and evaluation of potential forensic marker proteins in vaginal fluid by liquid chromatography/mass spectrometry

Development and Validation of Urine-based Peptide Biomarker Panels for Detecting Bladder Cancer in a Multi-center Study

<p>Dysregulation of SPRR3/miR-876-3p Axis Contributes to Tumorigenesis in Non-Small-Cell Lung Cancer</p>

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