Expression of ST13 in colorectal cancer and adjacent normal tissues

Wang, Linbo; Zhang, Suzhan; Peng, Jiaping; Feng, Yan; Fang, Shi-Chang; Wu, Jin-Min

doi:10.3748/wjg.v11.i3.336

Cited by 22 publications

(17 citation statements)

References 11 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…ST13 (suppression of tumorigenicity 13) maps to chromosome 22q13.2 and was reported to be down-regulated in CRC tissue when compared with its expression in adjacent normal tissue. 58 Bi-allelic mutations of p300, a transcriptional co-activator binding E1A also localized to chromosome 22q13.2, have been shown in several human cancers. In particular, this gene seems to function as a tumor suppressor gene in the intestinal epithelium, 59 and mutations in its coding region are found at high frequency among CRC cell lines.…”

Section: Discussionmentioning

confidence: 99%

Aneuploidy Arises at Early Stages of Apc-Driven Intestinal Tumorigenesis and Pinpoints Conserved Chromosomal Loci of Allelic Imbalance between Mouse and Human

Alberici

Pater

Cardoso

et al. 2007

The American Journal of Pathology

View full text Add to dashboard Cite

Although chromosomal instability characterizes the majority of human colorectal cancers, the contribution of genes such as adenomatous polyposis coli (APC), KRAS, and p53 to this form of genetic instability is still under debate. Here, we have assessed chromosomal imbalances in tumors from mouse models of intestinal cancer, namely Apc ؉/1638N , Apc ؉/1638N /KRAS V12G , and Apc ؉/1638N /Tp53 ؊/؊ , by array comparative genomic hybridization. All intestinal adenomas from Apc ؉/1638N mice displayed chromosomal alterations, thus confirming the presence of a chromosomal instability defect at early stages of the adenoma-carcinoma sequence. Moreover, loss of the Tp53 tumor suppressor gene, but not KRAS oncogenic activation, results in an increase of gains and losses of whole chromosomes in the Apc-mutant genetic background. Comparative analysis of the overall genomic alterations found in mouse intestinal tumors allowed us to identify a subset of loci syntenic with human chromosomal regions (eg, 1p34-p36, 12q24, 9q34, and 22q) frequently gained or lost in familial adenomas and sporadic colorectal cancers. The latter indicate that, during intestinal tumor development, the genetic mechanisms and the underlying functional defects are conserved across species. Hence, our array comparative genomic hybridization analysis of Apc-mutant intestinal tumors allows the definition of minimal aneuploidy regions conserved between mouse and human and likely to encompass rate-limiting genes for intestinal tumor initiation and progression.

show abstract

Section: Discussionmentioning

confidence: 99%

Aneuploidy Arises at Early Stages of Apc-Driven Intestinal Tumorigenesis and Pinpoints Conserved Chromosomal Loci of Allelic Imbalance between Mouse and Human

Alberici

Pater

Cardoso

et al. 2007

The American Journal of Pathology

View full text Add to dashboard Cite

show abstract

“…In our previous study, ST13 expression was lower in colorectal cancer tissues compared with the tumor-adjacent normal mucosa specimens (Wang et al, 2005). The previous study showed that ST13 re-expression mediated by oncolytic adenovirus within colorectal cancer cells can induce cell apoptosis and exert potent antitumour efficacy in colorectal cancer xenografts in nude mice.…”

Section: Discussionmentioning

confidence: 99%

“…In previous studies, we have proved that ST13 mRNA and protein levels were lower in colorectal cancer tissues compared with adjacent normal tissues (Mo et al, 1996;Zheng et al, 1997;Dong et al, 2005;Wang et al, 2005). Moreover, increased ST13 protein expression suppressed proliferation of colorectal cancer cells and induced apoptosis in colorectal cancer cell lines (Yang et al, 2008;Yu et al, 2009).…”

Section: Introductionmentioning

confidence: 99%

ST13, a proliferation regulator, inhibits growth and migration of colorectal cancer cell lines

Bai

Shi

Zhang

et al. 2012

J. Zhejiang Univ. Sci. B

View full text Add to dashboard Cite

Background and objective: ST13, is the gene encoding the HSP70 interacting protein (HIP). Previous research has shown that ST13 mRNA and protein levels are down-regulated in colorectal cancer (CRC) tissues compared with adjacent normal tissues. This study aims at the role of ST13 in the proliferation and migration of CRC cells. Methods: The transcript level of ST13 in different CRC cell lines was evaluated by quantitative reverse transcriptase-polymerase chain reaction (qRT-PCR). ST13-overexpressed and ST13-knockdown CRC cells were constructed respectively by lentiviral transduction, followed by 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide (MTT) assay, plate colony formation, cell-cycle analysis, and migration assays to evaluate the influence of ST13 on proliferation and migration in vitro. Moreover, a mouse xenograft study was performed to test in vivo tumorigenicity of ST13-knockdown CRC cells. Results: Lentivirus-mediated overexpression of ST13 in CRC cells inhibited cell proliferation, colony formation, and cell migration in vitro. In contrast, down-regulation of ST13 by lentiviralbased short hairpin RNA (shRNA) interference in CRC cells significantly increased cell proliferation and cloning efficiency in vitro. In addition, down-regulation of ST13 expression significantly increased the tumorigenicity of CRC cells in vivo. Conclusions: ST13 gene is a proliferation regulator that inhibits tumor growth in CRC and may affect cell migration.

show abstract

“…По данным литературы [21], ген ST13 ингибиру-ет опухолевый рост при различных видах рака. По-нижение экспрессии ST13 может приводить к риску развития карциномы желудка и колоректальной кар-циномы.…”

Section: результаты и обсуждениеunclassified

The role of genomic instability and expression of the p53 protein gene network in the processes of oncogenesis in first- and second-generation children living in radioactively contaminated areas

Балева¹,

Sukhorukov²,

Sipyagina³

et al. 2017

Ross. vestn. perinatol. pediatr.

View full text Add to dashboard Cite

Expression of ST13 in colorectal cancer and adjacent normal tissues

Abstract: The expression of ST13 decreases in colorectal cancer tissue compared with that in adjacent normal tissue. ST13 is mostly expressed in colorectal epithelia and adenocarcinoma cells.

Cited by 22 publications

References 11 publications

Aneuploidy Arises at Early Stages of Apc-Driven Intestinal Tumorigenesis and Pinpoints Conserved Chromosomal Loci of Allelic Imbalance between Mouse and Human

Aneuploidy Arises at Early Stages of Apc-Driven Intestinal Tumorigenesis and Pinpoints Conserved Chromosomal Loci of Allelic Imbalance between Mouse and Human

ST13, a proliferation regulator, inhibits growth and migration of colorectal cancer cell lines

The role of genomic instability and expression of the p53 protein gene network in the processes of oncogenesis in first- and second-generation children living in radioactively contaminated areas

Contact Info

Product

Resources

About