Expression of stem cell markers in stroma of odontogenic cysts and tumors

Chacham, M.; Almoznino, Galit; Zlotogorski‐Hurvitz, Ayelet; Buchner, Amos; Vered, Marilena

doi:10.1111/jop.13102

Cited by 11 publications

(24 citation statements)

References 42 publications

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“…A handful of studies investigated the role of SOX2 and OCT4 in this group of lesions [25][26][27][28]. The expression of SOX2 has been observed in the basal layer of DCs [27] and in the ameloblast-like cells as well as the stellate reticulum-like cells of AMs [16].…”

Section: Introductionmentioning

confidence: 99%

Expression of SOX2 and OCT4 in odontogenic cysts and tumors

et al. 2021

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Background Aberrant expression of stem cell markers has been observed in several types of neoplasms. This trait attributes to the acquired stem-like property of tumor cells and can impact patient prognosis. The objective of this study was to comparatively analyze the expression and significance of SOX2 and OCT4 in various types of odontogenic cysts and tumors. Methods Fifty-five cases of odontogenic cysts and tumors, including 15 ameloblastomas (AM), 5 adenomatoid odontogenic tumors (AOT), 5 ameloblastic fibromas (AF), 5 calcifying odontogenic cysts (COC), 10 dentigerous cysts (DC) and 15 odontogenic keratocysts (OKC) were investigated for the expression of SOX2 and OCT4 immunohistochemically. Results Most OKCs (86.7 %) and all AFs expressed SOX2 in more than 50 % of epithelial cells. Its immunoreactivity was moderate-to-strong in all epithelial cell types in both lesions. In contrast, SOX2 expression was undetectable in AOTs and limited to the ameloblast-like cells in a minority of AM and COC cases. Most DCs showed positive staining in less than 25 % of cystic epithelium. Significantly greater SOX2 expression was noted in OKC compared with DC or AM, and in AF compared with COC or AOT. OCT4 rarely expressed in odontogenic lesions with the immunoreactivity being mild and present exclusively in OKCs. Conclusions SOX2 is differentially expressed in odontogenic cysts and tumors. This could be related to their diverse cells of origin or stages of histogenesis. The overexpression of SOX2 and OCT4 in OKC indicates the acquired stem-like property. Future studies should investigate whether the overexpression of OCT4 and SOX2 contributes to the aggressive behaviors of the tumors.

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Section: Introductionmentioning

confidence: 99%

Expression of SOX2 and OCT4 in odontogenic cysts and tumors

et al. 2021

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“…OVOL1 mediates epidermal cell differentiation via suppressing EMT markers (SNAI2, ZEB2) and inducing epithelial markers (CDH1, ESRP1) 21 that were found downregulated and upregulated in OKC, respectively. Moreover, SOX2 is an ectodermal lineage specifier that is believed to promote the ability of OKC to adopt a stratified squamous epithelial fate, 22 and was found overexpressed in OKC in a previous microarray-based study. 3 Taken together, our observations suggest a developed epidermis-like phenotype in the OKC suprabasal epithelial cells, established in parallel to a significant upregulation of marker genes related to ESCs and cellular reprogramming (Figure 5).…”

Section: Discussionmentioning

confidence: 91%

Decoding a gene expression program that accompanies the phenotype of sporadic and basal cell nevus syndrome‐associated odontogenic keratocyst

Kalogirou

Foutadakis

Koutsi

et al. 2022

J Oral Pathology Medicine

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Background: Odontogenic keratocyst is characterized by local aggressive behavior and a high recurrence rate, as well as its potential to develop in association with the basal cell nevus syndrome. The aim of this study was to decode the gene expression program accompanying odontogenic keratocyst phenotype.Methods: 150-bp paired-end RNA-sequencing was applied on six sporadic and six basal cell nevus syndrome-associated whole-tissue odontogenic keratocyst samples in comparison to six dental follicles, coupled with bioinformatics and complemented by immunohistochemistry.Results: 2654 and 2427 differentially expressed genes were captured to characterize the transcriptome of sporadic and basal cell nevus syndrome-associated odontogenic keratocysts, respectively. Gene ontologies related to "epidermis/skin development" and "keratinocyte/epidermal cell differentiation" were enriched among the upregulated genes (KRT10, NCCRP1, TP63, GRHL3, SOX21), while "extracellular matrix organization" (ITGA5, LOXL2) and "odontogenesis" (MSX1, LHX8) gene ontologies were overrepresented among the downregulated genes in odontogenic keratocyst. Interestingly, upregulation of various embryonic stem cells markers (EPHA1, SCNN1A) and genes committed in cellular reprogramming (SOX2, KLF4, OVOL1, IRF6, TACSTD2, CDH1) was found in odontogenic keratocyst. These findings were highly shared between sporadic and basal cell nevus syndrome-associated odontogenic keratocysts. Immunohistochemistry verified SOX2, KLF4, OVOL1, IRF6, TACSTD2/TROP2, CDH1/E-cadherin, and p63 expression predominantly in the odontogenic keratocyst suprabasal epithelial layers. Conclusion:The odontogenic keratocyst transcriptomic profile is characterized by a prominent epidermal and dental epithelial fate, a repressed dental mesenchyme fate combined with deregulated extracellular matrix organization, and enhanced stemness gene signatures. Thus, we propose a developed epidermis-like phenotype in the odontogenic keratocyst suprabasal epithelial cells, established in parallel to a Marios Agelopoulos and Konstantinos I. Tosios have contributed equally to this work.

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“…The epithelial expression of NANOG has been described in head and neck cancers ( Lee et al, 2015 ; Rodrigues et al, 2017 ). Its expression was recently investigated in the stroma of odontogenic lesions, including AME, by Chacham et al (2020) , in which the presence of mesenchymal cells positive for NANOG was verified. Thus far, the present study is the only one to observe the expression of this protein in AME parenchyma.…”

Section: Discussionmentioning

confidence: 99%

“…Even more misleading would be to call them CSCs. The term “tumour stem cells” seems to be the most appropriate, since describing them as cells that have characteristics of SCs ( Monroy et al, 2018 ) or cells that express SC ( Chacham et al, 2020 ) markers seems somewhat vague.…”

Section: Discussionmentioning

confidence: 99%

Immunoexpression of stem cell markers SOX-2, NANOG AND OCT4 in ameloblastoma

Balbinot

Loureiro

Chemelo

et al. 2023

PeerJ

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Background Ameloblastoma (AME) is characterized by a locally invasive growth pattern. In an attempt to justify the aggressiveness of neoplasms, the investigation of the role of stem cells has gained prominence. The SOX-2, NANOG and OCT4 proteins are important stem cell biomarkers. Methodology To verify the expression of these proteins in tissue samples of AME, dentigerous cyst (DC) and dental follicle (DF), immunohistochemistry was performed and indirect immunofluorescence were performed on the human AME (AME-hTERT) cell line. Results Revealed expression of SOX-2, NANOG and OCT4 in the tissue samples and AME-hTERT lineage. Greater immunostaining of the studied proteins was observed in AME compared to DC and DF (p < 0.001). Conclusions The presence of biomarkers indicates a probable role of stem cells in the genesis and progression of AME.

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Expression of stem cell markers in stroma of odontogenic cysts and tumors

Cited by 11 publications

References 42 publications

Expression of SOX2 and OCT4 in odontogenic cysts and tumors

Expression of SOX2 and OCT4 in odontogenic cysts and tumors

Decoding a gene expression program that accompanies the phenotype of sporadic and basal cell nevus syndrome‐associated odontogenic keratocyst

Immunoexpression of stem cell markers SOX-2, NANOG AND OCT4 in ameloblastoma

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