Expression of stress proteins 70 in tilapia (<i>Oreochromis mossambicus</i>) during confinement and crowding stress

Dini, Luciana; Lanubile, Remigio; Tarantino, Patrizia; Mandich, Alberta; Cataldi, E.

doi:10.1080/11250000600679652

Cited by 19 publications

(5 citation statements)

References 31 publications

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“…Fishes are constantly exposed to stressful conditions in their natural habitats and hence, they respond to stressors by opening up a variety of stress-sensitive pathways [ 50 – 53 ]. These stressors can upset delicate balances in cellular metabolic responses, generate reactive oxygen species (ROS), and lead to oxidative stress and cellular damages [ 54 ].…”

Section: Discussionmentioning

confidence: 99%

Induction of Inducible Nitric Oxide Synthase by Lipopolysaccharide and the Influences of Cell Volume Changes, Stress Hormones and Oxidative Stress on Nitric Oxide Efflux from the Perfused Liver of Air-Breathing Catfish, Heteropneustes fossilis

Choudhury

Saha

2016

PLoS ONE

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The air-breathing singhi catfish (Heteropneustes fossilis) is frequently being challenged by bacterial contaminants, and different environmental insults like osmotic, hyper-ammonia, dehydration and oxidative stresses in its natural habitats throughout the year. The main objectives of the present investigation were to determine (a) the possible induction of inducible nitric oxide synthase (iNOS) gene with enhanced production of nitric oxide (NO) by intra-peritoneal injection of lipopolysaccharide (LPS) (a bacterial endotoxin), and (b) to determine the effects of hepatic cell volume changes due to anisotonicity or by infusion of certain metabolites, stress hormones and by induction of oxidative stress on production of NO from the iNOS-induced perfused liver of singhi catfish. Intra-peritoneal injection of LPS led to induction of iNOS gene and localized tissue specific expression of iNOS enzyme with more production and accumulation of NO in different tissues of singhi catfish. Further, changes of hydration status/cell volume, caused either by anisotonicity or by infusion of certain metabolites such as glutamine plus glycine and adenosine, affected the NO production from the perfused liver of iNOS-induced singhi catfish. In general, increase of hydration status/cell swelling due to hypotonicity caused decrease, and decrease of hydration status/cell shrinkage due to hypertonicity caused increase of NO efflux from the perfused liver, thus suggesting that changes in hydration status/cell volume of hepatic cells serve as a potent modulator for regulating the NO production. Significant increase of NO efflux from the perfused liver was also observed while infusing the liver with stress hormones like epinephrine and norepinephrine, accompanied with decrease of hydration status/cell volume of hepatic cells. Further, oxidative stress, caused due to infusion of t-butyl hydroperoxide and hydrogen peroxide separately, in the perfused liver of singhi catfish, resulted in significant increase of NO efflux accompanied with decrease of hydration status/cell volume of hepatic cells. However, the reasons for these cell volume-sensitive changes of NO efflux from the liver of singhi catfish are not fully understood with the available data. Nonetheless, enhanced or decreased production of NO from the perfused liver under osmotic stress, in presence of stress hormones and oxidative stress reflected its potential role in cellular homeostasis and also for better adaptations under environmental challenges. This is the first report of osmosensitive and oxidative stress-induced changes of NO production and efflux from the liver of any teleosts. Further, the level of expression of iNOS in this singhi catfish could also serve as an important indicator to determine the pathological status of the external environment.

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Section: Discussionmentioning

confidence: 99%

Induction of Inducible Nitric Oxide Synthase by Lipopolysaccharide and the Influences of Cell Volume Changes, Stress Hormones and Oxidative Stress on Nitric Oxide Efflux from the Perfused Liver of Air-Breathing Catfish, Heteropneustes fossilis

Choudhury

Saha

2016

PLoS ONE

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“…The optimum temperature of the fish is nonlethally manipulated by the farmers around ±10°C. This practice enhances tilapia immunity by producing heat shock proteins (Dini et al, 2006;Chen et al, 2014). Additionally, farmers use ducks as biological controls that feed on debris on the production net to enhance water circulation and improve dissolved oxygen.…”

Section: Management Practices For Fish and Lake Healthmentioning

confidence: 99%

Understanding tilapia mortalities and fish health management in Lake Volta: a systematic approach

Zornu,

Tavornpanich,

Brun

et al. 2023

Front. Sustain. Food Syst.

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Unusual fish mortalities in aquaculture threaten global food security and carry significant socio-economic burdens. In 2018, Nile tilapia (Oreochromis niloticus) suffered unusual patterns of mortalities, attributed to disease-causing agents in Lake Volta cage aquaculture. In recent times, disease investigations have shifted from single to consideration of multiple factors to understand the puzzling range of causal risk factors. This study therefore aimed at expanding on tilapia mortality risk factors, while documenting fish health and Lake Volta management practices for sustainable aquaculture. We interviewed relevant aquaculture stakeholders operating on Lake Volta and conducted thematic analysis on their responses to map out mortality risk factors and management practices. The identified risk factors were conceptualized in established models of causation web and Social-Ecological System to explain the practical significance of the findings. The results showed that the risk factors of tilapia mortalities are a combination of pathogens and non-infectious factors mediated by weak law enforcement. The results further suggested mortality reinforcing mechanisms through the horizontal transmission of pathogens, namely, Streptococcus agalactiae and Infectious Spleen and Kidney Necrosis Virus. Moreover, the recognition of weak enforcement as a possible factor reinforcing human activities is a non-infectious route that can be deleterious to fish health. Health management practices comprised phytotherapy, vaccination, heat shock treatment, biological controls, and best husbandry practices. Lake management involves creating a waterfront buffer of 85.34 m, surveillance, and executing the framework guiding aquaculture development on the Lake. The findings are suggestive of complementary quantitative studies that augment the qualitative evidence herein. Such follow up studies can disclose precise mortality risk factors to inform policy directives and effective remedial strategies that can secure fish and lake health.

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“…1999). Confinement and crowding increase Hsp70 in the liver, heart and gills of tilapia (Dini et al. 2006), whereas in sea bass mRNA encoding Hsp70, but not Hsp90, accumulates with crowding (Gornati et al.…”

Section: Inducers Of Heat Shock Proteins In Aquatic Organismsmentioning

confidence: 99%

Stress response for disease control in aquaculture

Sung

MacRae

Sorgeloos

et al. 2011

Reviews in Aquaculture

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Heat shock proteins (Hsps) are robustly induced by diverse stressors that denature proteins. In addition to stress resistance, Hsps are involved in the folding of nascent proteins, plant and animal development, aging, environmental adaptation and the immune response, demonstrating the fundamental importance of these proteins to cell survival. Heat shock proteins are induced in aquatic organisms by perturbations of temperature and salinity, environmental contaminants, handling, hormones and biotic stressors. Exposure to sublethal stress may enhance tolerance to a subsequent stress, a process termed induced thermotolerance, and provide protection to stressors other than the initial stress, known as cross-tolerance. In the present review, we briefly describe the established approaches that are used to control disease during aquaculture. This is followed by documentation of Hsp induction after exposure to stressors commonly encountered by aquatic organisms. Induced thermotolerance, cross-tolerance and immune enhancement by Hsps are also considered. Although physiological stress is known to decrease disease resistance it is now becoming clear that stress-induced Hsps enhance the tolerance of aquatic organisms to disease. Potential applications for Hsps in the commercial production of fish, crustaceans and molluscs are indicated, an issue of significance when the importance of aquaculture in feeding the world's population is realized

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Expression of stress proteins 70 in tilapia (Oreochromis mossambicus) during confinement and crowding stress

Cited by 19 publications

References 31 publications

Induction of Inducible Nitric Oxide Synthase by Lipopolysaccharide and the Influences of Cell Volume Changes, Stress Hormones and Oxidative Stress on Nitric Oxide Efflux from the Perfused Liver of Air-Breathing Catfish, Heteropneustes fossilis

Induction of Inducible Nitric Oxide Synthase by Lipopolysaccharide and the Influences of Cell Volume Changes, Stress Hormones and Oxidative Stress on Nitric Oxide Efflux from the Perfused Liver of Air-Breathing Catfish, Heteropneustes fossilis

Understanding tilapia mortalities and fish health management in Lake Volta: a systematic approach

Stress response for disease control in aquaculture

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