Expression of stress-related genes in tomato plants exposed to arsenic and chromium in nutrient solution

Abstract

International audienceThe molecular responses of hydroponically cultivated tomato plants to As(V) or Cr(VI) were assessed by transcript accumulation analysis of genes coding for products potentially involved in heavy metal tolerance. A quantitative real-time PCR experiment was performed with Hsp90-1, MT2- and GR1-like protein genes using RNA isolated from tomato roots or shoots treated for 24 h with As(V) or Cr(VI) at concentrations ranging from 80 to 640 μM. Both transient metallic treatments induced Hsp90-1 transcript accumulation in tomato plants. MT2- and GR1-like transcripts accumulated in tomato roots treated with As(V) but were only slightly affected by Cr(VI) treatment. Tomatoes showed phenotypic symptoms to heavy metal toxicity when plants were exposed to Cr(VI) but not As(V). Plant lethality was observed at 1280 μM Cr(VI), indicating that tomatoes were more tolerant to As than Cr stress under the experimental conditions used here

Relationships among lipid peroxidation, SOD enzyme activity, and SOD gene expression profile in Lycopersicum esculentum L. exposed to cold stress

“…Overexpression of antioxidant genes, such as GR, APX, SOD, etc., have been demonstrated to provide enhanced tolerance to ROS-induced stress in several crop species (Roxas et al, 2000;Shinozaki and Yamaguchi-Shinozaki, 2000;Payton et al, 2001;Dai et al, 2009;Goupil et al, 2009;Fortunato et al, 2010). Different gene encoding factors involved in signal transduction cascades, such as transcription factors, are induced by stress treatments, which in turn might affect expression patterns of antioxidant genes (Shinozaki and Yamaguchi-Shinozaki, 1996).…”

Análisis de expresión de tres genes relacionados a estrés en respuesta a un exceso de boro en Solanum lycopersicum cv Poncho Negro

“…Transgenic tobacco seedlings over-expressing this rHsp90 showed tolerance to high salt concentrations (Liu et al 2006). Its activity is also induced by metals such as cadmium, chromium and arsenic (Goupil et al 2009), confirming its important roles in multiple environmental stresses.…”

“…Our results suggest that Poncho Negro tomato detects B-stress and activates mechanisms that alleviate the cell damage induced and preserve cell homeostasis in roots and leaves. The presence of a high concentration of arsenic (As) also activates MT2 expression in roots and leaves of tomato (Goupil et al 2009). The presence of high concentrations of As in irrigation water (0.1 -0.6 mg/lt) and soil (197.5 -254.7 mg/g) (DGA, 2004) in the Lluta Valley allow us to suggest that Poncho Negro tomato may also be able to tolerate As stress.…”

Variations of Plant Macronutrients and Secondary Metabolites Content in Response to Radionuclides Accumulation

“…These mechanisms involve reduced metal uptake, oxidative defense, metal chelation, repair of stress-damaged proteins and vacuolar compartmentalization [14]. Many nutritional factors are widely considered to be critical for human health.…”