Expression of Sucrose Transporters from Vitis vinifera Confer High Yield and Enhances Drought Resistance in Arabidopsis

Abstract

Sucrose is the predominant form of sugar transported from photosynthetic (source) to non-photosynthetic (sink) organs in higher plants relying on the transporting function of sucrose transporters (SUTs or SUCs). Many SUTs have been identified and characterized in both monocots and dicots. However, the function of sucrose transporters (SUTs or SUCs) from Vitis is not clear. As the world's most planted grape species, Vitis vinifera owns three sucrose transport activity verified SUTs. In this study, we constructed three kinds of VvSUC (Vitis vinifera SUC)-overexpressing transgenic Arabidopsis. VvSUC-overexpressing transgenic Arabidopsis was cultured on sucrose-supplemented medium. VvSUC11and VvSUC12-overexpressing lines had similar thrived growth phenotypes, whereas the size and number of leaves and roots from VvSUC27-overexpressing lines were reduced compared with that of WT. When plants were cultured in soil, all SUT transgenic seedlings produced more number of leaves and siliques, resulting in higher yield (38.6% for VvSUC12-transformants) than that of WT. Besides, VvSUC27-transformants and VvSUC11-transformants enhanced drought resistance in Arabidopsis, providing a promising target for crop improvement

Hetero/Homo-Complexes of Sucrose Transporters May Be a Subtle Mode to Regulate Sucrose Transportation in Grape Berries

“…The extract solutions were diluted with ddH 2 O to a final volume of 100 mL. Measurements were performed using an anthrone-sulfuric acid colorimetric assay, as previously described in [ 39 ].…”

The Molecular Regulation of Carbon Sink Strength in Grapevine (Vitis vinifera L.)

“…VvSUC27 is a SUT located at the plasma membrane and belongs to the dicot-specific SUT1 subfamily, which includes the remaining AtSUCs ( Cai et al, 2017 ). Overexpression of VvSUCs ( VvSUC11 or VvSUC12 , or VvSUC27 ) in both tobacco and Arabidopsis conferred more rapid development, higher yield and enhanced abiotic stress resistance ( Cai et al, 2017 , 2020 ). Similarly, Cai et al (2019) reported SUTs in ‘Zuoshan-1’ ( Vitis vinifera ) responded to various stress stimuli and subsequently promoted sucrose metabolism and hormone synthesis.…”

Sucrose Metabolism and Transport in Grapevines, with Emphasis on Berries and Leaves, and Insights Gained from a Cross-Species Comparison

“…The activity of sugar transporter proteins is subject to regulation [118,119]; however, very little is known about this in grapevines [11]. Expression studies in amenable species can provide useful insights into transporter function [76,120]. In order to understand function further, it is necessary to determine in which cell types a transporter is present, and few such studies have been performed in grape [2,11,121].…”