Expression of surfactant Protein-A in the Haemophilus influenzae-induced otitis media in a rat model

Yu, Gun Hee; Kim, Hee-Bok; Ko, Seo Hyun; Kim, Youn Woo; Lim, Yun-Sung; Park, Seok-Won; Cho, Chang Gun; Park, Joo Hyun

doi:10.1016/j.ijporl.2018.06.030

Cited by 8 publications

(5 citation statements)

References 34 publications

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“…found that SP-A was up-regulated in rat ME after induction of OM by NTHi and that expression of SP-A was also identified in the ME effusion of humans. 33…”

Section: Discussionmentioning

confidence: 99%

Innate immunity of surfactant protein A in experimental otitis media

Abdel-Razek

Yang

et al. 2019

Innate Immun

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Surfactant protein A (SP-A) plays an important role in innate immune response and host defense against various microorganisms through opsonization and complement activation. To investigate the role of SP-A in non-typeable Haemophilus influenzae (NTHi)-induced acute otitis media, this study used wild type C57BL/6 (WT) and SP-A knockout (KO) mice. We divided mice into an infection group in which the middle ear (ME) was injected with NTHi and a control group that received the same treatment using normal saline. Mice were sacrificed on d 1, 3, and 7 after treatment. Temporal bone samples were fixed for histological, cellular, and molecular analyses. Ear washing fluid (EWF) was collected for culture and analyses of pro-inflammatory cytokines and inflammatory cells. SP-A-mediated bacterial aggregation and killing and phagocytosis by macrophages were studied in vitro. SP-A expression was detected in the ME and Eustachian tube mucosa of WT mice but not KO mice. After infection, KO mice showed more severe inflammation evidenced by increased ME mucosal thickness and inflammatory cell infiltration and higher NF-κB activation compared to WT mice. The levels of IL-6 and IL-1β in the EWF of infected KO mice were higher compared to infected WT mice on d 1. Our studies demonstrated that SP-A mediated NTHi aggregation and killing and enhanced bacterial phagocytosis by macrophages in vitro and modulated inflammation of the ME in otitis media in vivo.

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“…found that SP-A was up-regulated in rat ME after induction of OM by NTHi and that expression of SP-A was also identified in the ME effusion of humans. 33…”

Section: Discussionmentioning

confidence: 99%

Innate immunity of surfactant protein A in experimental otitis media

Abdel-Razek

Yang

et al. 2019

Innate Immun

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“…After overnight incubation at 37 °C, the mixture was centrifuged to obtain a pellet. A bacterial suspension of 1 × 10 5 NTHi cells/mL was used in the experiment 4 , 9 , 30 . All the rats with normal tympanic membranes were anesthetized via intraperitoneal injection of an anesthetic mixture of Rompun (Bayer, Leverkusen, Germany) and Zoletil (Virbac, Texas, USA) at a dose of 1 mL/kg.…”

Section: Methodsmentioning

confidence: 99%

“…The soft tissue was dissected and both bullae were exposed. A 25-gauge needle was used to pierce a hole at the bulla tip, and 0.05 mL of the bacterial suspension was injected into the hole 4 , 9 , 30 . The same anesthetic solution was used on days 0, 2, 7, and 10 after inoculation when acquiring photographs of the tympanic membrane to check the progression of OM.…”

Section: Methodsmentioning

confidence: 99%

Effects of cigarette smoke on Haemophilus influenzae-induced otitis media in a rat model

Choi

Kang

et al. 2021

Sci Rep

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Exposure to cigarette smoke (CS) is a factor that could delay or worsen the recovery of otitis media (OM) by causing inflammatory swelling of the Eustachian tube (ET). However, despite the suggested relationship, little is known about the association between OM and CS. Therefore, we aimed to evaluate the effects of CS on the development, progression, and recovery of OM, as well as the histological and molecular changes caused by CS exposure, by using a rat model of OM infected with non-typeable Haemophilus influenzae (NTHi). Eighty Sprague–Dawley rats with normal middle ears (MEs) were divided into four groups (n = 20 rats/group): control, CS, OM, and CS + OM. The CS and CS + OM groups were exposed to CS for 2 weeks. The inflammatory reaction to NTHi was more intense and lasted longer in the CS + OM group than in the other groups. Goblet cell proliferation and mucus secretion in the ET were more significant in the CS and CS + OM groups than in the other groups. These findings suggested that because CS directly affects the ET and ME mucosa, bacterial OM can become more severe and may resolve more slowly in the presence of CS exposure rather than in its absence.

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“…Mice knocked out for SP‐A are susceptible to infections by common airway microbes, 59 and SP‐A can increase the phagocytosis of Streptococcus pneumoniae and Hemophilus influenzae , 60 which are common pathogens in otitis media. SP‐A is also upregulated in the response of lipopolysaccharide local injection in experimental animal models of otitis media, 61 and genetic polymorphisms of SP‐A may be associated with various risks of developing the disease 62,63 . Surfactant protein‐B and various phospholipids are also expressed in the eustachian tube; therefore, all main components of pulmonary surfactant are produced in this tissue by fully competent cells having a complete surfactant machinery 64,65 …”

Section: Possible Extrapulmonary Surfactant Indicationsmentioning

confidence: 99%

Extrapulmonary Surfactant Therapy: Review of Available Data and Research/Development Issues

Foligno

Loi

Pezza

et al. 2020

The Journal of Clinical Pharma

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Since the discovery of surfactant, a large amount of knowledge has been accumulated about its biology and pharmacology. Surfactant is the cornerstone of neonatal respiratory critical care, but its proteins and phospholipids are produced in various tissues and organs, with possible roles only partially similar to that played in the alveoli. As surfactant research is focused mainly on its respiratory applications, knowledge about the possible role of surfactant in extrapulmonary disorders has never been summarized. Here we aim to comprehensively review the data about surfactant biology and pharmacology in organs other than the lung, especially focusing in the more promising surfactant extrapulmonary roles. We also review any preclinical or clinical data available about the therapeutic use of surfactant in these contexts. We offer a summary of knowledge and research/development milestones, as possible useful guidance for researchers of multidisciplinary background.

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Expression of surfactant Protein-A in the Haemophilus influenzae-induced otitis media in a rat model

Cited by 8 publications

References 34 publications

Innate immunity of surfactant protein A in experimental otitis media

Innate immunity of surfactant protein A in experimental otitis media

Effects of cigarette smoke on Haemophilus influenzae-induced otitis media in a rat model

Extrapulmonary Surfactant Therapy: Review of Available Data and Research/Development Issues

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