Expression of target genes by coinfection with replication-deficient viral vectors.

Yap, Chan-Choo; Ishii, Koji; Aizaki, Hideki; Tani, Hideki; Aoki, Yasunori; Ueda, Yoshiaki; Matsuura, Yoshiharu; Miyamura, Tatsuo

doi:10.1099/0022-1317-79-8-1879

Cited by 12 publications

(6 citation statements)

References 47 publications

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“…In pT7HCV07Luc, the luciferase gene was connected in frame to the coding region of two-thirds of the core protein. pHCV09Luc, which has been described previously (61), has nt 1 to 924 of the HCV cDNA, the luciferase gene, cDNA for the coding region of the C terminus of NS5B and the 3ЈUTR of HCV, a ribozyme of hepatitis D virus, and a T7 terminator under the T7 promoter. In this plasmid, the luciferase gene was connected in frame to the E1 coding gene.…”

Section: Cellsmentioning

confidence: 99%

Interaction of Hepatitis C Virus Core Protein with Viral Sense RNA and Suppression of Its Translation

Shimoike

Mimori

Tani

et al. 1999

J Virol

148

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To clarify the binding properties of hepatitis C virus (HCV) core protein and its viral RNA for the encapsidation, morphogenesis, and replication of HCV, the specific interaction of HCV core protein with its genomic RNA synthesized in vitro was examined in an in vivo system. The positive-sense RNA from the 5′ end to nucleotide (nt) 2327, which covers the 5′ untranslated region (5′UTR) and a part of the coding region of HCV structural proteins, interacted with HCV core protein, while no interaction was observed in the same region of negative-sense RNA and in other regions of viral and antiviral sense RNAs. The internal ribosome entry site (IRES) exists around the 5′UTR of HCV; therefore, the interaction of the core protein with this region of HCV RNA suggests that there is some effect on its cap-independent translation. Cells expressing HCV core protein were transfected with reporter RNAs consisting of nt 1 to 709 of HCV RNA (the 5′UTR of HCV and about two-thirds of the core protein coding regions) followed by a firefly luciferase gene (HCV07Luc RNA). The translation of HCV07Luc RNA was suppressed in cells expressing the core protein, whereas no significant suppression was observed in the case of a reporter RNA possessing the IRES of encephalomyocarditis virus followed by a firefly luciferase. This suppression by the core protein occurred in a dose-dependent manner. The expression of the E1 envelope protein of HCV or β-galactosidase did not suppress the translation of both HCV and EMCV reporter RNAs. We then examined the regions that are important for suppression of translation by the core protein and found that the region from nt 1 to 344 was enough to exert this suppression. These results suggest that the HCV core protein interacts with viral genomic RNA at a specific region to form nucleocapsids and regulates the expression of HCV by interacting with the 5′UTR.

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Section: Cellsmentioning

confidence: 99%

Interaction of Hepatitis C Virus Core Protein with Viral Sense RNA and Suppression of Its Translation

Shimoike

Mimori

Tani

et al. 1999

J Virol

148

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“…However, it is suggested that the transduction block lies not in the endosomal escape, but in the cytoplasmic trafficking or nuclear import of the virus capsids WI38 [21] MRC5 [132,147] ECV-304 [113] HUVEC [148] PC3 (prostate cancer) [149] Osteosarcoma SAOS-2 [21,150] Glioma cells [45] Pancreatic cells [151] Keratinocytes [21] Bone marrow fibroblast [21] Primary foreskin fibroblast [152] Primary neural cells [115] Primary hepatocytes [7,8] Coronary smooth muscle cells and coronary endothelial cells [12] Mesenchymal stem cells [14] Embryonic stem cells [16] Non-human primate cells COS-7 [21,30] Vero [109] CV-1 [20] Porcine cells CPK [119] FS-L3 [9] coronary smooth muscle cell [12] Bovine cells…”

Section: A Mechanismsmentioning

confidence: 99%

Baculoviral Vectors for Gene Delivery: A Review

2008

CGT

132

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Baculovirus has emerged as a novel vector for in vivo and in vitro gene delivery. In addition, the applications of baculovirus-mediated gene transfer have been explosively expanded to drug screening, eucaryotic gene display, cancer therapy and tissue engineering, etc. The capability of baculovirus viral envelope for protein/peptide display also renders itself a potential vaccine delivery platform. This paper reviews the history, factors influencing baculovirus-mediated gene delivery and emerging in vitro, in vivo and ex vivo applications. Efforts aimed at overcoming current existing bottlenecks and recent progresses in addressing the safety concerns are particularly emphasized.

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“…Yap and colleagues [46] reported the use of a two baculovirus system to recover high-titer polio virus from cDNA. [50][51][52] For example, baculovirus HBV chimeras were used to evaluate the antiviral properties of a nucleoside analog (lamivudine) on HBVreplication in HepG2 cells. [50][51][52] For example, baculovirus HBV chimeras were used to evaluate the antiviral properties of a nucleoside analog (lamivudine) on HBVreplication in HepG2 cells.…”

Section: Studies Of Viral Infectionsmentioning

confidence: 99%