Expression of tetraspanins in human lung cancer cells: frequent downregulation of CD9 and its contribution to cell motility in small cell lung cancer

Funakoshi, Tadanao; Tachibana, Isao; Hoshida, Yoshihiko; Kimura, Hiromi; Takeda, Yoshito; Kijima, Takashi; Nishino, Kazumi; Goto, Hiroyuki; Yoneda, Takunari; Kumagai, Toru; Osaki, Tadashi; Hayashi, Seiji; Aozasa, Katsuyuki; Kawase, Ichiro

doi:10.1038/sj.onc.1206106

Cited by 82 publications

(85 citation statements)

References 42 publications

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“…TM4SF1) previously reported by our laboratory (11) and the plasmolipin gene previously reported by Pass et al (12). In other studies, low expression of CD9 is thought to contribute to a more aggressive (metastatic) phenotype in small cell lung cancer (19), gastric cancer (20), and breast cancer (21). These observations are generally consistent with our finding of CD9 expressed at significantly higher levels in tumors from patients with relatively good prognosis.…”

Section: Discussionsupporting

confidence: 83%

Validation of Genomics-Based Prognostic Tests in Malignant Pleural Mesothelioma

Gordon¹,

Rockwell

Godfrey³

et al. 2005

Clinical Cancer Research

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Purpose: Malignant pleural mesothelioma (MPM) is a highly lethal neoplasm with limited pretreatment prognostication strategies. In this report, we examine the accuracy of a previously proposed prognostic test in an independent cohort of MPM patients. This test uses simple ratios of gene expression levels to provide a novel prognostication scheme. Experimental Design: Gene expression data using high-density oligonucleotide microarrays (f22,000 genes) were obtained for a new cohort of human MPM tumors from patients undergoing similar treatments (n = 39). The relative expression levels for specific genes were also determined using real-time quantitative reverse transcription-PCR. We also used a subset of these tumors associated with widely divergent patient survival (n = 23) as a training set to identify new treatment-specific candidate prognostic molecular markers and gene ratio^based prognostic tests. The predictive nature of these newly discovered markers and gene ratiob ased prognostic tests were then examined in an independent group of tumors (n = 52) using microarray data and quantitative reverse transcription-PCR. Results: Previously described MPM prognostic genes and gene ratio^based prognostic tests predicted clinical outcome in 39 independent MPM tumor specimens in a statistically significant manner. Newly discovered treatment-specific prognostic genes and gene ratio^based prognostic tests were highly accurate and statistically significant when examined in an independent group of 52 tumors from patients undergoing similar treatment. Conclusions:The data support the use of gene ratios in translating gene expression data into easily reproducible, statistically validated clinical tests for the prediction of outcome in MPM.

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Section: Discussionsupporting

confidence: 83%

Validation of Genomics-Based Prognostic Tests in Malignant Pleural Mesothelioma

Gordon¹,

Rockwell

Godfrey³

et al. 2005

Clinical Cancer Research

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“…Deregulation of these receptors has been implicated in various types of cancers Hendrix et al, 2003), but has never been identified as having a possible role in NF1. Other genes already known to be involved in the progression of other cancer types, such as CD9 (Funakoshi et al, 2003), are also deregulated significantly in T265 cells (Table 1). CD9 has been implicated in lymph node metastasis (Kusukawa et al, 2001) and in the invasive and metastatic phenotype of small lung cell cancer (Miyake et al, 1999).…”

Section: Cell Adhesion Moleculesmentioning

confidence: 95%

Transcriptional profiling in an MPNST-derived cell line and normal human Schwann cells

et al. 2004

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AbstractcDNA microarrays were utilized to identify abnormally expressed genes in a malignant peripheral nerve sheath tumor (MPNST)-derived cell line, T265, by comparing the mRNA abundance profiles with that of normal human Schwann cells (nhSCs). The findings characterize the molecular phenotype of this important cell-line model of MPNSTs, and elucidate the contribution of Schwann cells in MPNSTs. In total, 4608 cDNA sequences were screened and hybridizations replicated on custom cDNA microarrays. In order to verify the microarray data, a large selection of differentially expressed mRNA transcripts were subjected to semi-quantitative reverse transcription PCR (LightCycler). Western blotting was performed to investigate a selection of genes and signal transduction pathways, as a further validation of the microarray data. The data generated from multiple microarray screens, semi-quantitative RT-PCR and Western blotting are in broad agreement. This study represents a comprehensive gene-expression analysis of an MPNST-derived cell line and the first comprehensive global mRNA profile of nhSCs in culture. This study has identified ~900 genes that are expressed abnormally in the T265 cell line and detected many genes not previously reported to be expressed in nhSCs. The results provide crucial information on the T265 cells that is essential for investigation using this cell line in experimental studies in neurofibromatosis type I (NF1), and important information on normal human Schwann cells that is applicable to a wide range of studies on Schwann cells in cell culture.

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“…Assessment of VDR and DBP in lung cancer cell lines and normal lung tissue Cancer cell lines were described in our previous work and cultured as indicated therein [19]. NCI-231 was originally a gift to I. Tachibana from Y. Shimosata (National Cancer Research Institute, Tokyo, Japan) in 2003.…”

Section: Prognostic Effect Of Serum Markers Of the Vitamin D Axismentioning

confidence: 99%

“…HARA was purchased from the Health Sciences Research Resource Center (Tokyo, Japan), again in 2003. All cells were tested prior to the experiments herein for neural cell adhesion molecular expression by flow cytometry (either positive or negative; data not shown) and mycoplasma infection (all negative), as described in our previous work [19].…”

Section: Prognostic Effect Of Serum Markers Of the Vitamin D Axismentioning

confidence: 99%

Circulating DBP level and prognosis in operated lung cancer: an exploration of pathophysiology

Turner¹,

McGowan²,

Millen³

et al. 2012

Eur Respir J

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Vitamin D stimulates transcription of antiangiogenic and apoptotic factors that may suppress tumours, while vitamin D binding protein (DBP) may be a biomarker in murine lung cancer models. We sought to ascertain whether the vitamin D axis is altered in lung cancer or influences prognosis.148 lung cancer patients, 68 other intrathoracic cancer patients and 33 noncancer controls were studied for up to 5 yrs. Circulating DBP and vitamin D levels were compared between groups and their effect on survival assessed by Cox regression analysis. Expression of DBP and vitamin D receptor (VDR) was examined in lung cancer cell lines and in normal and tumour lung tissue by Western blot and immunohistochemistry.Low serum DBP levels predicted lung cancer-specific death (p50.04), and DBP was poorly expressed in lung cancer cells on Western blot and immunohistochemistry. Vitamin D did not predict cancer survival and VDR expression was variable in tumours.Preservation of serum DBP is a significant independent factor associated with better cancer outcome in operated lung cancer patients. Given the established role of DBP in macrophage activation and clearance of abnormal cells, further study on its involvement in lung cancer is merited.

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Expression of tetraspanins in human lung cancer cells: frequent downregulation of CD9 and its contribution to cell motility in small cell lung cancer

Cited by 82 publications

References 42 publications

Validation of Genomics-Based Prognostic Tests in Malignant Pleural Mesothelioma

Validation of Genomics-Based Prognostic Tests in Malignant Pleural Mesothelioma

Transcriptional profiling in an MPNST-derived cell line and normal human Schwann cells

Circulating DBP level and prognosis in operated lung cancer: an exploration of pathophysiology

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