Expression of Th2-skewed pathology mediators in monocyte-derived type 2 of dendritic cells (DC2)

Hata, Mieko; Takahara, Seiji; Tsuzaki, Hidetoshi; Ishii, Yoshiki; Nakata, Koh; Akagawa, Kiyoko S.; Satoh, Kaneo

doi:10.1016/j.imlet.2009.07.008

Cited by 20 publications

(18 citation statements)

References 59 publications

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“…Hata et al . [9] succeeded in making mDC1s and mDC2s from freshly isolated circulating monocytes using either GM-CSF with IL-4 or IL-3 with IL-4, respectively. Furthermore, they confirmed the potential of CD1a as a marker for mDC1s through the differences in expression between mDC1 and mDC2 subsets.…”

Section: Discussionmentioning

confidence: 99%

“…In a previous study, Th1-inducible DC subtype differentiated from peripheral monocytes by cultivation with GM-CSF expressed higher levels of CD1a than CD1c [9]. Based on these data, we use CD1a as a better marker of mDC1s.…”

Section: Introductionmentioning

confidence: 91%

“…However, recent studies have led to a new theory that both DC1s and DC2s differentiate from mDCs, and in some reports, the terms mDC1 and mDC2 are used to describe the mDC subsets which promote Th1 response and Th2 response, respectively. In fact, mDC1s can be generated by cultivation of monocytes with granulocyte macrophage colony-stimulating factor (GM-CSF) and IL-4, whereas mDC2s can be generated by cultivation of monocytes with IL-3 and IL-4 [8,9]. …”

Section: Introductionmentioning

confidence: 99%

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Comparative analysis of circulating dendritic cell subsets in patients with atopic diseases and sarcoidosis

et al. 2013

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BackgroundDendritic cells (DCs) are professional antigen-presenting cells that play a crucial role in the initiation and modulation of immune responses. Human circulating blood DCs are divided into two major subsets: myeloid DCs (mDCs); and plasmacytoid DCs (pDCs). Furthermore, mDCs are subdivided into two subsets: Th1-promoting mDCs (mDC1s); and Th2-promoting mDCs (mDC2s). Although CD1a, CD1c, and CD141 are generally used for classifying mDC subsets, their adequacy as a specific marker remains unclear. We performed this study to compare circulating mDC, pDC, mDC1, and mDC2 subsets between Th1- and Th2-mediated diseases using CD1a and CD141, and to analyze the adequacy of CD1a and CD141 as a marker for mDC1s and mDC2s, respectively.MethodsThirty patients with sarcoidosis, 23 patients with atopic diseases, such as atopic bronchial asthma, and 23 healthy subjects as controls were enrolled in this study. Peripheral blood DC subsets were analyzed with flow cytometry according to expressions of CD11c, CD123, CD1a, and CD141. For functional analysis, we measured interleukin (IL) 12p40 levels produced by the sorted mDC subsets.ResultsThe sarcoidosis group showed decreased total DC (P < 0.05) and mDC counts (P < 0.05) compared to controls. The atopy group showed decreased CD1a+mDC count (P < 0.05), and increased CD1a-mDC count (P < 0.05) compared to controls. CD141+mDC count in the atopy group was higher than controls (P < 0.05). Sorted CD1a+mDCs produced higher levels of IL-12p40 than CD1a-mDCs (P = 0.025) and CD141+mDCs (P = 0.018).ConclusionsWe conclude that decreased count of CD1a+mDC and increased count of CD141+mDC may reflect the Th2-skewed immunity in atopic diseases. The results of IL-12 levels produced by the sorted mDC subsets suggested the adequacy of CD1a and CD141 as a marker for mDC1 and mDC2, respectively, in vivo.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 91%

Section: Introductionmentioning

confidence: 99%

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Comparative analysis of circulating dendritic cell subsets in patients with atopic diseases and sarcoidosis

et al. 2013

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“…Viperin knockout mice show an impaired Th2 response and T-cell receptor mediated activation of NF- κB and AP-1 [46]. In another mouse study, it was shown that viperin is expressed at higher levels in Th2 cells compared to Th1 cells [47]. These results suggest that viperin may play an important role in the immune system.…”

Section: Discussionmentioning

confidence: 99%

Viperin mRNA is a novel target for the human RNase MRP/RNase P endoribonuclease

Mattijssen

Hinson

Onnekink

et al. 2010

Cell. Mol. Life Sci.

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RNase MRP is a conserved endoribonuclease, in humans consisting of a 267-nucleotide RNA associated with 7–10 proteins. Mutations in its RNA component lead to several autosomal recessive skeletal dysplasias, including cartilage-hair hypoplasia (CHH). Because the known substrates of mammalian RNase MRP, pre-ribosomal RNA, and RNA involved in mitochondrial DNA replication are not likely involved in CHH, we analyzed the effects of RNase MRP (and the structurally related RNase P) depletion on mRNAs using DNA microarrays. We confirmed the upregulation of the interferon-inducible viperin mRNA by RNAi experiments and this appeared to be independent of the interferon response. We detected two cleavage sites for RNase MRP/RNase P in the coding sequence of viperin mRNA. This is the first study providing direct evidence for the cleavage of a mRNA by RNase MRP/RNase P in human cells. Implications for the involvement in the pathophysiology of CHH are discussed.Electronic supplementary materialThe online version of this article (doi:10.1007/s00018-010-0568-3) contains supplementary material, which is available to authorized users.

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“…In addition, difficulty in maintaining differentiated primary intestinal M cells in culture, as well as the lack of in vitro models, has delayed progress in the understanding of M cell physiology and function (Niedergang and Kraehenbuhl, 2000). Dendritic cells (DCs) and cytotoxic T lymphoCorrespondence to: Won-Bong Park, College of Natural Sciences, Seoul Women's University, Seoul 139-774, Korea Tel: 82-2-970-5655, Fax: 82-2-975-3159 E-mail: wbpark@swu.ac.kr cytes (CTLs) are the first protecting barrier against different pathogens (viruses, bacteria and neoplasms cells) (Krawczyk et al, 2006;Hata et al, 2009). Mature DCs synthesize high levels of IL-12 (Inaba et al, 1990;Steinman and Banchereau, 1998;Fernandez et al, 2002) that enhance both innate (natural killer cells) and acquired (B and T cells) immunity.…”

Section: Introductionmentioning

confidence: 99%

Mistletoe lectin transport by M-cells in follicle-associated epithelium (FAE) and IL-12 secretion in dendritic cells situated below FAE In Vitro

Lyu

Park

2010

Arch. Pharm. Res.

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A galactose- and N-acetyl-D-galactosamine-specific lectin (Viscum album L. var. coloratum agglutinin, VCA), which is known for its anti-tumor activity, was isolated from Korean mistletoe. Mistletoe preparations have been given subcutaneously because of the unstability and poor absorption in the GI tract. In this study, we investigated the effect of incubation time and glucose on the VCA transport across the in vitro model of human FAE (follicle-associated epithelium) by two different cell models: Caco-2 cell monolayers mimicking human enterocytes and a model of the human FAE which is mainly composed of M-cells and enterocytes. As a result, the VCA transport across the FAE monolayer cells was higher and faster than the transport across the Caco-2 monolayer cells, and glucose increased VCA transport across both monolayer cells. In addition, IL-12 was secreted from myeloid DC1 and lymphoid DC2.4 cells which were co-treated simultaneously with LPS and VCA. Furthermore, the FAE model associated with underlying immune cells was established and VCA was added to the inserts apically. There was a greater IL-12 secretion in dendritic cells situated below FAE monolayer than Caco-2 monolayer. The results from this study provide important insight into the possible oral application of mistletoe on anti-tumor therapeutics.

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Expression of Th2-skewed pathology mediators in monocyte-derived type 2 of dendritic cells (DC2)

Cited by 20 publications

References 59 publications

Comparative analysis of circulating dendritic cell subsets in patients with atopic diseases and sarcoidosis

Comparative analysis of circulating dendritic cell subsets in patients with atopic diseases and sarcoidosis

Viperin mRNA is a novel target for the human RNase MRP/RNase P endoribonuclease

Mistletoe lectin transport by M-cells in follicle-associated epithelium (FAE) and IL-12 secretion in dendritic cells situated below FAE In Vitro

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