Expression of the aphthovirus rna polymerase gene in Escherichia coli and its use together with other bioengineered nonstructural antigens in detection of late persistent infections

Neitzert, E.; Beck, Ewald; Mello, P Augé de; Gomes, I.; Bergmann, I

doi:10.1016/0042-6822(91)90456-l

Cited by 77 publications

(22 citation statements)

References 28 publications

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“…The association of small amounts of 3D with purified FMDV virions has been reported [170] and may contribute to the immunogenicity of 3D in vaccinated animals. The availability of other NSP, expressed in different systems, has recently allowed identification of new serological markers of FMDV infection [142,143,168,187]. In particular, promising results have been reported with ELISA based on the detection of 3AB-3ABC antibodies [64,187,223].…”

Section: Diagnosis and Viral Characterisationmentioning

confidence: 93%

Foot-and-mouth disease virus: a long known virus, but a current threat

et al. 2001

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-Foot-and-mouth disease virus (FMDV) was the first animal virus identified. Since then, FMDV has become a model system in animal virology and a considerable amount of information on its structure, biology and vaccinology has been obtained. However, the disease that this virus produces (FMD) still constitutes one of the main animal health concerns. In this review, we have attempted to summarise the state of the knowledge in different basic and applied areas of FMDV research, with emphasis on those aspects relevant to the control of the disease. FMDV / structure / immunity / vaccine / variability / diagnosisRésumé -Le virus de la fièvre aphteuse : un virus connu de longue date, qui demeure une menace. Le virus de la fièvre aphteuse a été le premier virus animal identifié. Depuis lors, il est devenu un système modèle en virologie animale, et une quantité importante d'informations sur sa structure, sa biologie et sa vaccinologie a été obtenue. Cependant la maladie provoquée par ce virus constitue encore une inquiétude majeure en santé animale. Dans cette revue, nous avons tenté de résumer l'état des connaissances dans différents domaines de recherche, à la fois fondamentaux et appliqués, sur le virus de la fièvre aphteuse, en mettant l'accent sur les aspects relatifs au contrôle de la maladie. virus de la fièvre aphteuse / immunité / vaccin / variabilité / diagnostic

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Section: Diagnosis and Viral Characterisationmentioning

confidence: 93%

Foot-and-mouth disease virus: a long known virus, but a current threat

et al. 2001

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“…This may partially explain the high degree of NS1 conservation among influenza A virus strains (5,19,30) and provides an antigenic marker for influenza virus infections (4,22). Nonstructural protein has also been used as a marker for hepatitis C virus (15) and foot-andmouth disease virus infections (20).…”

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confidence: 99%

Diagnostic Approach for Differentiating Infected from Vaccinated Poultry on the Basis of Antibodies to NS1, the Nonstructural Protein of Influenza A Virus

et al. 2005

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Vaccination programs for the control of avian influenza (AI) in poultry have limitations due to the problem of differentiating between vaccinated and virus-infected birds. We have used NS1, the conserved nonstructural protein of influenza A virus, as a differential diagnostic marker for influenza virus infection. Experimentally infected poultry were evaluated for the ability to induce antibodies reactive to NS1 recombinant protein produced in Escherichia coli or to chemically synthesized NS1 peptides. Immune sera were obtained from chickens and turkeys inoculated with live AI virus, inactivated purified vaccines, or inactivated commercial vaccines. Seroconversion to positivity for antibodies to the NS1 protein was achieved in birds experimentally infected with multiple subtypes of influenza A virus, as determined by enzyme-linked immunosorbent assay (ELISA) and Western blot analysis. In contrast, animals inoculated with inactivated gradient-purified vaccines had no seroconversion to positivity for antibodies to the NS1 protein, and animals vaccinated with commercial vaccines had low, but detectable, levels of NS1 antibodies. The use of a second ELISA with diluted sera identified a diagnostic test that results in seropositivity for antibodies to the NS1 protein only in infected birds. For the field application phase of this study, serum samples were collected from vaccinated and infected poultry, diluted, and screened for anti-NS1 antibodies. Field sera from poultry that received commercial AI vaccines were found to possess antibodies against AI virus, as measured by the standard agar gel precipitin (AGP) test, but they were negative by the NS1 ELISA. Conversely, diluted field sera from AI-infected poultry were positive for both AGP and NS1 antibodies. These results demonstrate the potential benefit of a simple, specific ELISA for anti-NS1 antibodies that may have diagnostic value for the poultry industries.

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“…Also the number of epitopes found on such a long recombinant protein could result in unspecific reactions, because of cross-reactivities with antibodies to other picornaviruses (10).…”

mentioning

confidence: 99%

Identification of Foot-and-Mouth Disease Virus-Specific Linear B-Cell Epitopes To Differentiate between Infected and Vaccinated Cattle

Höhlich¹,

Wiesmüller

Schlapp

et al. 2003

J Virol

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Foot-and-mouth disease (FMD) is a highly contagious viral disease of cloven-hoofed animals. For several years, vaccination of animals, which had proven to be successful for the eradication of the disease, has been forbidden in the United States and the European Community because of the difficulty of differentiating between vaccinated and infected animals. In this study, detailed investigations of the bovine humoral immune response against FMD virus (FMDV) were performed with the aim of identifying viral epitopes recognized specifically by sera derived from FMDV-infected animals. The use of overlapping 15-mer synthetic peptides, covering the whole open reading frame of FMDV strain O 1 K in a peptide enzyme-linked immunosorbent assay, allowed the identification of 12 FMDV strain O 1 K-specific linear B-cell epitopes. Six of these linear B-cell epitopes, located in the nonstructural proteins, were used in further assays to compare the reactivities of sera from vaccinated and infected cattle. Antibodies recognizing these peptides could be detected only in sera derived from infected cattle. In further experiments, the reactivity of the six peptides with sera from animals infected with different strains of FMDV was tested, and strain-independent infection-specific epitopes were identified. Thus, these results clearly demonstrate the ability of a simple peptide-based assay to discriminate between infected and conventionally FMD-vaccinated animals.

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Expression of the aphthovirus rna polymerase gene in Escherichia coli and its use together with other bioengineered nonstructural antigens in detection of late persistent infections

Cited by 77 publications

References 28 publications

Foot-and-mouth disease virus: a long known virus, but a current threat

Foot-and-mouth disease virus: a long known virus, but a current threat

Diagnostic Approach for Differentiating Infected from Vaccinated Poultry on the Basis of Antibodies to NS1, the Nonstructural Protein of Influenza A Virus

Identification of Foot-and-Mouth Disease Virus-Specific Linear B-Cell Epitopes To Differentiate between Infected and Vaccinated Cattle

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