Expression of the common acute lymphoblastic leukemia antigen (CALLA) on the surface of individual cells of human lymphoblastoid lines.

Abstract

Expression of the common acute lymphoblastic leukemia antigen (CALLA) on the surface of individual cells of the human lymphoblastoid lines CW678, Namalwa, and Nalm-6, and the distribution of the antigen epitopes within the cell populations have been determined quantitatively with the murine monoclonal anti-CALLA antibody J5. The distribution of CALLA epitopes in the cell populations was analyzed by indirect immunofluorescence measured by using flow cytometry. The average number of CALLA epitopes per cell were measured by two assays: in a direct assay by binding 125I-labeled antibody J5 to cells, and indirectly by binding 125I-labeled protein A from Staphylococcus aureus to J5-coated cells. On average, CW678, Namalwa, and Nalm-6 cells bore about 1 X 10(4), 6 X 10(4), and 8 X 10(4) CALLA epitopes per cell respectively. Histograms of the absolute number of CALLA epitopes expressed by individual cells in the populations of CW678, Namalwa, and Nalm-6 cultures were generated by a combined analysis of all the binding data. This is the first example of histograms showing quantitative distribution of antigen epitopes. Previously, the expression of antigens by individual cells as obtained by flow cytometry was only presented in terms of relative fluorescence intensity of individual cells in cell populations.

CD14−CD10−CD45+HLA‐DR−SSC+ neutrophils may be granulocytic myeloid‐derived suppressor cell–like cells and relate to disease progression in non‐Hodgkin's lymphoma patients

“…The cell-surface glycoprotein CD10 is mainly expressed on lymphoid progenitors and terminal myeloid cells (neutrophils) in the hematologic system. [6][7][8] Previous studies have suggested that the function of CD10 in neutrophils may be related to various inflammatory responses. 28,29 However, in patients with NHL, whether the change in CD10 expression on the neutrophil surface could indicate a functional transformation, such as translation to G-MDSC-like morphological and functional characteristics, is rarely reported.…”

“…The cell‐surface glycoprotein CD10 is mainly expressed on lymphoid progenitors and terminal myeloid cells (neutrophils) in the hematologic system 6–8 . Previous studies have suggested that the function of CD10 in neutrophils may be related to various inflammatory responses 28,29 .…”

“…Moreover, CD10 was originally identified in acute lymphoblastic leukemia cells or the common acute lymphoblastic leukemia antigen; therefore, the protein is typically used as a cell surface marker to differentiate and identify hematological malignancies. [5][6][7] Later studies discovered that CD10 is widely expressed in normal lymphoid progenitors, neutrophils and various malignant cells. The relationship between CD10 expression in circulating neutrophils and tumors is complex and needs to be clarified.…”

“…Cluster of differentiation 10 (CD10) in humans is a 90–110 kDa type II integral membrane protein. Moreover, CD10 was originally identified in acute lymphoblastic leukemia cells or the common acute lymphoblastic leukemia antigen; therefore, the protein is typically used as a cell surface marker to differentiate and identify hematological malignancies 5–7 . Later studies discovered that CD10 is widely expressed in normal lymphoid progenitors, neutrophils and various malignant cells.…”