Expression of the CTCF gene in bovine oocytes and preimplantation embryos

Rios, Álvaro Fabrício Lopes; Lemos, Daniela Cristina; Fernandes, Mauricio Barros; Andréa, Maria Carolina da Silva; Gomes, M.V.; Lôbo, Raysildo Barbosa; Mazucato, Mendelson; Ramos, Ester Silveira

doi:10.1590/s1415-47572007000600029

Cited by 4 publications

(1 citation statement)

References 18 publications

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“…The amplification products (328 bp for the first set of primers, and 153 bp for the second) were analyzed by electrophoresis in 8% polyacrylamide gels stained with 2% silver nitrate. As a DNA positive control, we used a bovine β-actin gene sequence previously described (Rios et al, 2007).…”

Section: Molecular Analysismentioning

confidence: 99%

Sexing single bovine blastomeres using TSPY gene amplification

Carneiro

Takeuchi²,

Araújo³

et al. 2011

Genet. Mol. Res.

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ABSTRACT. The testis-specific protein Y-encoded gene (TSPY) is a Y-specific gene present in variable copy number in many mammalian species, including cattle. We tested the applicability of the TSPY gene as a Y-specific marker to predict preimplantation embryo sex in Nelore (Bos indicus) cattle. Two blastomeres were removed from each embryo. A total of 36 single blastomeres and the remaining cells of their 18 matched in vitro conceived embryos were screened for TSPY amplification by nested-PCR. The results obtained from a single blastomere and the remaining cells of the same embryo were concordant in all cases. All blastomeres (16/16) from eight embryos produced with sexed sperm (specific for production of male embryos) were TSPY-positive. We conclude that TSPY is a good male-specific marker, the usefulness of which is probably enhanced by the high copy number. Other methods that are less time-consuming, such as real-time PCR, could be improved with the use of the TSPY gene sequences to generate primers and/or probes. This is the first report to demonstrate the applicability of the TSPY gene for sexing single cells in cattle.

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Section: Molecular Analysismentioning

confidence: 99%