Expression of the Cutaneous Lymphocyte-Associated Antigen in Circulating T Cells in Drug-Allergic Reactions

Abstract

Background: Mechanisms underlying the production of delayed cutaneous reactions to drugs are poorly characterized. The cutaneous lymphocyte-associated antigen (CLA) is a skin-homing T cell receptor that defines T lymphocytes associated with the cutaneous immune response. We studied the percentage and activation phenotype of circulating CLA+ T cells in drug allergic patients and healthy controls. Methods: PBMCs were isolated from heparinized venous blood by Ficoll density gradient. Lymphocytes were stained for flow cytometry with anti-CLA, anti-CD3 and anti-HLA-DR mAbs. Five-parameter analysis was performed on an Ortho Cytoron Absolute flow cytometer. Results: We found increased percentages of circulating CLA+ T cells in drug- allergic patients compared to controls. Moreover, CLA+ T cells from drug-allergic individuals expressed a higher percentage of the T cell activation marker HLA-DR. Conclusions: These results suggest that CLA+ T cells may play a role in the pathology of delayed cutaneous reactions to drugs. Further studies are in progress to elucidate the role of skin-homing T cells in allergic reactions to drugs.

Peripheral Blood CD4 T-Cell and Plasmacytoid Dendritic Cell (pDC) Reactivity to Herpes Simplex Virus 2 and pDC Number Do Not Correlate with the Clinical or Virologic Severity of Recurrent Genital Herpes

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An Extremely Diverse CD4 Response to Vaccinia Virus in Humans Is Revealed by Proteome-Wide T-Cell Profiling

“…Gating selected cells in the lymphocyte forward/ side scatter region. Selected cultures were stimulated with UV-inactivated cellassociated HSV-2 strain 333 as described elsewhere (32). For the IFN-␥ ELISPOT we used our published procedures (47).…”

Dominance and Diversity in the Primary Human CD4 T Cell Response to Replication-Competent Vaccinia Virus

“…Cells were stained with anti-CD4-PE (BD Biosciences) on day 3 and 5 ϫ 10 4 cells in the lymphoblast forward/side scatter gate were analyzed. For PBMC, IFN-␥ responses were measured using 3 ϫ 10 6 PBMC incubated with UV-mock or UV-vaccinia (1/1000) in 1 ml of TCM with anti-CD28 and anti-CD49d (BD Biosciences) (33). Brefeldin A (Sigma-Aldrich) was added after 1 h. After 6 h, cells were stained with anti-CD4-PE-cyanin 5 or FITC, permeabilized, and stained with anti-IFN-␥-PE or isotype-PE control (BD Biosciences) (26).…”