Expression of the cyclin-dependent kinase 2 gene (cdk2) influences ovarian development in the ridgetail white prawn, Exopalaemon carinicauda

Pan, Zuanfeng; Hua, Songsong; Li, Yongchuang; Zhang, Simian; Li, Xue; Shi, Tingting; Wang, Panpan; Yan, Binlun; Li, Jitao; Gao, Huan

doi:10.1016/j.aqrep.2022.101265

Cited by 14 publications

(6 citation statements)

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“…This may be due to the division of many mesodermal and ectodermal cells before nodal larva. The cell division was slow after nauplius (Zhang et al, 2022a). Thus, the present study suggests that Ec-cyclin B and Ec-cdc2 were jointly involved in regulating the embryonic development of E. carinicauda.…”

Section: Discussionmentioning

confidence: 49%

“…In our study, the Ec-cyclin B expression was the highest in the blastocyst stage, significantly higher than in other periods (p < 0.05), gradually decreasing with embryonic development. Maybe due to the enhanced cell cycle regulation activities brought on by the continual division of embryonic cells, the expression of genes involved in cell cycle regulation tends to rise as oosperm develops into nauplius (Zhang et al, 2022a). The expression of Mn-cyclin B mRNA was higher in the embryonic development stage than in the larval and post-larval development stages and the highest in the cleavage stage, suggesting that cyclin B played an essential role in the embryogenesis of M. nipponense (Zhang et al, 2022c).…”

Section: Discussionmentioning

confidence: 99%

“…However, the artificial large-scale breeding technology of E. carinicauda has not been resolved yet, due to the ovaries of different individuals developing asynchronously. The juvenile E. carinicauda are mainly obtained from natural sea areas (Li et al, 2019;Liang et al, 2020;Zhang et al, 2022aZhang et al, , 2022b. A need exists for a clearer understanding of the reproduction processes in this shrimp to provide the necessary background knowledge to support E. carinicauda farming.…”

Section: Introductionmentioning

confidence: 99%

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Profiles of cyclin B and cdc2 during ovarian and embryonic development in Exopalaemon carinicauda

Liang

Sun

et al. 2023

Israeli Journal of Aquaculture - Bamidgeh

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Mitosis-promoting factor (MPF) is a complex formed by cyclin B (cyclin B) and cyclin-dependent kinase (cdc2). To investigate the role of MPF in the reproduction of Exopalaemon carinicauda, we cloned its full-length cDNA of the Ec-cyclin B and Ec-cdc2 genes. We analyzed their molecular characteristics and expression profiles during ovarian and embryonic development. The results showed that the Ec-cyclin B gene was 1194 bp long and encoded a 397 amino acid (aa) long protein. However, Ec-cdc2 was 900 bp long, which encoded 299 aa with a conserved cyclin binding motif PSTAIRE. The phylogenetic tree analysis showed that Ec-cyclin B had the highest homology with the cyclin B of Macrobrachium rosenbergii (81.06%). In comparison, Ec-cdc2 had the highest homology with the cdc2 of E. modestus (96.80%). Ec-cyclin B showed the highest expression in the ovary, whereas Ec-cdc2 was the highest in the hepatopancreas, followed by the ovary. In the five stages of ovarian development, Ec-cyclin B and Ec-cdc2 expression levels reach the highest at stage Ⅴ(p < 0.05). Overall, the expression of these two genes first increased and then decreased at different embryonic developmental stages. Therefore, these findings suggested that cyclin B and cdc2 played an essential role in the ovarian and embryonic development of E. carinicauda.

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Section: Discussionmentioning

confidence: 49%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Profiles of cyclin B and cdc2 during ovarian and embryonic development in Exopalaemon carinicauda

Liang

Sun

et al. 2023

Israeli Journal of Aquaculture - Bamidgeh

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“…The ridgetail white prawn, Exopalaemon carinicauda (Holthuis, 1950) [currently also again referred to as Palaemon carinicauda Holthuis, 1950], is one of the most important economic crustacean species in China because of its fast growth, strong adaptability, delicious taste and rich nutritional value (Wang et al, 2021;Lai et al, 2022b;Zhang et al, 2022). Many studies have been carried out on the shrimp model with regard to its breeding and aquaculture (Wang et al, 2021;Lai et al, 2022a;Pang et al, 2022), and its physiology, biochemistry, and gene functioning (Gao et al, 2017(Gao et al, , 2021Ma et al, 2020;Shen et al, 2021;Lai et al, 2022b).…”

Section: Introductionmentioning

confidence: 99%

Isolation and characterization of the actinomycete, Nocardiopsis, from Haizhou Bay, and its application as a probiotic for Exopalaemon carinicauda (Holthuis, 1950) (Caridea, Palaemonidae)

Shen

Shen³

et al. 2023

Crustac.

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Actinomycetes, as the main producers of a variety of active metabolites and enzymes, have received extensive attention in aquaculture. An actinomycete had been isolated from Haizhou Bay and been identified by morphological analysis and 16S rRNA sequencing. Results showed that the actinomycete had the highest similarity (99.65%) with Nocardiopsis sp. and was named strain E10. Strain E10 produced an exo-enzyme with proteinase effect that showed strong antibacterial activity against Vibrio anguillarum. This strain E10 was tested as a probiotic for the culture of Exopalaemon carinicauda. After having been fed with a diet supplemented with strain E10 for 30 days, the final body weight (Wt), weight gain rate (WGR), special growth rate (SGR), survival rate (SR) and digestive enzymes (chymotrypsin, α-amylase and lipase) of E. carinicauda in all experimental groups showed no significant differences (). However, the antioxidant enzymes (total superoxide dismutase, catalase and peroxidase) and the non-specific immune-related enzymes (acid phosphatase, alkaline phosphatase and lysozyme) in the treatment groups increased significantly () compared with the control group. The cumulative mortality of the treatment groups (35.56% in 5 × 109 CFU/100 g) was significantly lower than that of the control treatment (73.33%) () post challenge with V. anguillarum for 14 days. The results indicated that strain E10 had no significant effect on the growth, but a significant improvement on the immunity of E. carinicauda. This study provides the data for ecological aquaculture of E. carinicauda.

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“…In the ovarian development of shrimp, members of the Cdk gene family are involved in the regulation of oocyte maturation and ovulation. For example, Cyclin-dependent kinase 2 gene (cdk2) plays an important role in the proliferation of oocytes and the synthesis and transportation of exogenous yolk material [7]. The cyclin gene family is also involved in the ovarian development of shrimp.…”

Section: Introductionmentioning

confidence: 99%

Molecular Markers of Ovarian Germ Cells of Banana Prawn (Fenneropenaeus merguiensis)

Sengseng,

Okutsu,

Songnui

et al. 2023

CIMB

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The banana prawn (Fenneropenaeus merguiensis) is a valuable prawn in the worldwide market. However, cultivation of this species is limited owing to the difficulty in culture management and limited knowledge of reproduction. Therefore, we studied the gene expression and molecular mechanisms involved in oogenesis for elucidating ovarian germ cell development in banana prawns. The tissue-specific distribution of certain genes identified from previous transcriptome data showed that FmCyclinB, FmNanos, and nuclear autoantigenic sperm protein (FmNASP) were only expressed in gonads. The in situ hybridization (ISH) of these three genes showed different expression patterns throughout oogenesis. FmCyclinB was highly expressed in pre-vitellogenic oocytes. FmNanos was expressed at almost the same level during oogenesis but showed the most expression in late pre-vitellogenic stages. Based on the highest expression of FmCyclinB and FmNanos in mid pre-vitellogenic and late pre-vitellogenic oocytes, respectively, we suggested that FmNanos may suppress FmCyclinB expression before initiation of vitellogenesis. Meanwhile, FmNASP expression was detected only in pre-vitellogenesis. Moreover, quantitative real-time polymerase chain reaction (qRT-PCR) analysis of FmNASP expression was supported by FmNASP ISH analysis based on high expression of FmNASP in sub-adult ovaries, which contain most of pre-vitellogenic oocytes. In this study, we found three reliable ovarian markers for banana prawns and also found a dynamic change of molecular mechanism during the sub-stage of pre-vitellogenesis. We determined the expression levels of these genes involved in oogenesis. Our findings provide information for further studies on banana prawn reproduction which may assist in their cultivation.

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Expression of the cyclin-dependent kinase 2 gene (cdk2) influences ovarian development in the ridgetail white prawn, Exopalaemon carinicauda

Cited by 14 publications

References 30 publications

Profiles of cyclin B and cdc2 during ovarian and embryonic development in Exopalaemon carinicauda

Profiles of cyclin B and cdc2 during ovarian and embryonic development in Exopalaemon carinicauda

Isolation and characterization of the actinomycete, Nocardiopsis, from Haizhou Bay, and its application as a probiotic for Exopalaemon carinicauda (Holthuis, 1950) (Caridea, Palaemonidae)

Molecular Markers of Ovarian Germ Cells of Banana Prawn (Fenneropenaeus merguiensis)

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