Expression of the developmental markers STRO-1 and alkaline phosphatase in cultures of human marrow stromal cells: regulation by fibroblast growth factor (FGF)-2 and relationship to the expression of FGF receptors 1–4

Walsh, S.; Jefferiss, Carolyn M.; Stewart, Karina; Jordan, Grant; Screen, Joanne; Beresford, J.N.

doi:10.1016/s8756-3282(00)00319-7

Cited by 112 publications

(75 citation statements)

References 45 publications

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“…The only exception was that some of the RS-1 and RS-2 cells were positive for CD4, which is expressed on thymocytes and peripheral blood lymphocytes, including T cells. Also of interest was that both the RS-1 and RS-2 cells were negative for STRO-1, an epitope originally suggested as a marker for MSCs (30,31). However, some of the mMSCs contained the STRO-1 epitope, an observation consistent with their ability to differentiate into osteoblasts (21).…”

Section: Differentiation Of Rs Cells and Mmscsmentioning

confidence: 89%

Identification of a subpopulation of rapidly self-renewing and multipotential adult stem cells in colonies of human marrow stromal cells

Colter

Sekiya

Prockop

2001

Proc. Natl. Acad. Sci. U.S.A.

826

675

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Marrow stromal cells are adult stem cells from bone marrow that can differentiate into multiple nonhematopoietic cell lineages. Previous reports demonstrated that single-cell-derived colonies of marrow stromal cells contained two morphologically distinct cell types: spindle-shaped cells and large flat cells. Here we found that early colonies also contain a third kind of cell: very small round cells that rapidly self-renew. Samples enriched for the small cells had a greater potential for multipotential differentiation than samples enriched for the large cells. Also, the small cells expressed a series of surface epitopes and other proteins that potentially can be used to distinguish the small cells from the large cells. The results suggested it will be important to distinguish the major subpopulations of marrow stromal cells in defining their biology and their potential for cell and gene therapy.B one marrow contains at least two kinds of stem cells, hematopoietic stem cells and stem cells for nonhematopoietic tissues (1-26), variously referred to as mesenchymal stem cells or marrow stromal cells (MSCs). MSCs are of interest because they are easily isolated from a small aspirate of bone marrow and readily generate single-cell-derived colonies (1-5, 25, 27); the single-cell-derived colonies can be expanded through as many as 50 population doublings in about 10 weeks (25), and they can differentiate into osteoblasts, adipocytes, chondrocytes (1-13), myocytes (9), astrocytes, oligodendrocytes, and neurons (17,23,26,27). For these reasons, the cells are currently being tested for their potential use in cell and gene therapy for a number of human diseases (22,24). Previous reports (3,11,12) demonstrated that single-cell-derived colonies of human MSCs are heterogeneous in that they contain at least two morphologically distinct kinds of cells: spindle-shaped cells and large cuboidal or flattened cells. Here we have extended our previous observations (25) to demonstrate that the colonies also contain extremely small cells that are rapidly self-renewing (RS cells). The RS cells appear to be the earliest progenitors in the cultures and have the greatest potential for multilineage differentiation. They differ from more mature cells in the same cultures by a series of surface epitopes and expressed proteins. MethodsIsolation and Growth of MSCs. To isolate human MSCs, bone marrow aspirates of 10-20 ml were taken from the iliac crest of normal donors ranging in age from 19 to 49 years old under an Institutional Review Board approved protocol. Nucleated cells were isolated with a density gradient [Ficoll͞Paque (Pharmacia)] and resuspended in complete culture medium [␣ MEM, GIBCO͞BRL; 20% FBS, lot-selected for rapid growth of MSCs (Atlanta Biologicals); 100 units/ml of penicillin͞100 g/ml of streptomycin͞2 mM L-glutamine (GIBCO͞BRL)]. All of the cells were plated in 25 ml of medium in a 175-cm 2 culture dish (Falcon) and incubated at 37°C with 5% humidified CO 2 . After 24 h, nonadherent cells were discarded, and adherent cells ...

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Section: Differentiation Of Rs Cells and Mmscsmentioning

confidence: 89%

Identification of a subpopulation of rapidly self-renewing and multipotential adult stem cells in colonies of human marrow stromal cells

Colter

Sekiya

Prockop

2001

Proc. Natl. Acad. Sci. U.S.A.

826

675

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“…(Kawaguchi et al, 1994;Nakamura et al, 1997;Inui et al, 1998;Kawaguchi et al, 2001, Chen et al, 2004. Although the mechanism has not been fully elucidated, it has the ability to stimulate angiogenesis (Montesano et al, 1986;Hayek et al, 1987;Collin-Osdoby et al, 2002) and, the proliferation and differentiation of osteoblasts (Globus et al, 1988;Nakamura et al, 1995;Walsh et al, 2000;Pun et al, 2001) to possibly aid the repair of bone fractures.…”

Section: Angiogenesis and Bone Fracture Repairmentioning

confidence: 99%

“…Disruption of the normal bone vasculature can result in skeletal problems such as osteopetrosis (Aharinejad et al, 1995;Aharinejad et al, 1999), metastatic bone disease (Taichman et al, 2002) and inflammatory bone loss in rheumatoid arthritis and periodontal disease Walsh and Mapp, 1998;Schwartz et al, 2000). Thus, we are now beginning to understand the intimate relationship between the vasculature and bone, and the important role played in maintaining bone homeostasis, offering the possibility of novel new approaches to solving the problems of fracture healing, particularly in delayed and non-union pathologies.…”

Section: Angiogenesis and Bone Fracture Repairmentioning

confidence: 99%

Osteogenesis and angiogenesis: The potential for engineering bone

Kanczler

Oreffo²

2008

eCM

895

637

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The repair of large bone defects remains a major clinical orthopaedic challenge. Bone is a highly vascularised tissue reliant on the close spatial and temporal connection between blood vessels and bone cells to maintain skeletal integrity. Angiogenesis thus plays a pivotal role in skeletal development and bone fracture repair. Current procedures to repair bone defects and to provide structural and mechanical support include the use of grafts (autologous, allogeneic) or implants (polymeric or metallic). These approaches face significant limitations due to insufficient supply, potential disease transmission, rejection, cost and the inability to integrate with the surrounding host tissue.

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“…Alp and Ocn play crucial roles as phenotypic markers for early and later stage bone formation, and increased Alp and Ocn expression correlates with increased bone formation. 36 As well as this, RunX2 has been identified as the primary transcription factor for osteoblastic differentiation. 37 In vitro studies have shown that RunX2 positively controls Ocn expression.…”

mentioning

confidence: 88%

Antimicrobial and osteogenic effect of Ag-implanted titanium with a nanostructured surface

Zheng

Li²,

Liu³

et al. 2012

IJN

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Ag-implanted titanium with a nanostructured surface was prepared by hydrothermal treatment with H 2 O 2 followed by Ag plasma immersion ion implantation. Streptococcus mutans, Porphyromonas gingivalis and Candida albicans were chosen for antimicrobial tests. Genes related to microbial structure or adhesion, namely glucan-binding proteins B (GbpB), fimbria protein A (FimA), and agglutinin-like sequence4 (Als4), were examined. The osteoblast's attachment, viability, and quantitative analysis of osteogenic gene expression (Alp, Ocn, RunX2) on titanium surfaces were evaluated. Scanning electron microscopy (SEM) revealed that Ag nanoparticles of approximately 10 nm were incorporated on the nanostructured surface of titanium after Ag plasma immersion ion implantation. Trials showed that 93.99% of S. mutans, 93.57% of P. g, and 89.78% of C. albicans were killed on the Ag-implanted titanium with a nanostructured surface. Gene expressions from the three microorganisms confirmed the antimicrobial activities of the Ag-implanted titanium with a nanostructured surface. Furthermore, the adhesive images and viability assays indicated that the Ag-implanted titanium with a nanostructured surface did not impair osteoblasts. The expressions of osteoblast phenotype genes in cells grown on the Ag-implanted titanium surface were significantly increased. The results of this study suggest that the Ag-implanted titanium with a nanostructured surface displays good antimicrobial properties, reducing gene expressions of microorganisms, and excellent cell adhesion and osteogenic effects.

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Expression of the developmental markers STRO-1 and alkaline phosphatase in cultures of human marrow stromal cells: regulation by fibroblast growth factor (FGF)-2 and relationship to the expression of FGF receptors 1–4

Cited by 112 publications

References 45 publications

Identification of a subpopulation of rapidly self-renewing and multipotential adult stem cells in colonies of human marrow stromal cells

Identification of a subpopulation of rapidly self-renewing and multipotential adult stem cells in colonies of human marrow stromal cells

Osteogenesis and angiogenesis: The potential for engineering bone

Antimicrobial and osteogenic effect of Ag-implanted titanium with a nanostructured surface

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