Expression of the DMRT gene and its roles in early gonadal development of the Japanese pufferfish Takifugu rubripes

Yamaguchi, Akihiko; Lee, Kyung Hoon; Fujimoto, Hiromi; Kadomura, Kazushi; Yasumoto, Susumu; Matsuyama, Michiya

doi:10.1016/j.cbd.2005.08.003

Cited by 64 publications

(70 citation statements)

References 29 publications

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“…A fragment of dmrt1 which encodes the DM domain of 46 AA (ABF57693) had been reported with testis specific expression in G. rarus detected by RT-PCR (Zhang et al, 2008 expressed in both the ovary and testes in a sexually dimorphic pattern: high expression in the testes, low in the ovary. This result is similar to findings in other fish such as zebrafish (Guo et al, 2005), rainbow trout (Oncorhynchus mykiss) (Marchand et al, 2000), pufferfish (Takifugu rubripes) (Yamaguchi et al, 2006) and southern catfish (Silurus meridionals) (Liu et al, 2007). Strong male-biased expression of dmrt1 may be the general rule (Herpin and Schartl, 2011), although male-restricted expression of dmrt1 has been reported in North African catfish (Clarias gariepinus) (Raghuveer and Senthilkumaran, 2009), Nile tilapia (Oreochromis niloticus) and Japanese ricefish (Oryzias latipes) (Kobayashi et al, 2004).…”

Section: Sexually Dimorphic Expression Of Dmrt1 In G Rarussupporting

confidence: 73%

Sexually dimorphic and ontogenetic expression of dmrt1, cyp19a1a and cyp19a1b in Gobiocypris rarus

Cao

Duan

Cheng

et al. 2012

Comparative Biochemistry and Physiology Part A: Molecular &

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Section: Sexually Dimorphic Expression Of Dmrt1 In G Rarussupporting

confidence: 73%

Sexually dimorphic and ontogenetic expression of dmrt1, cyp19a1a and cyp19a1b in Gobiocypris rarus

Cao

Duan

Cheng

et al. 2012

Comparative Biochemistry and Physiology Part A: Molecular &

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“…DMRT1 is the master sex-determining gene in animals with ZW sex determination systems, including chickens and Cynoglossus semilaevis (53). Studies on DMRT1 genes have been performed in medaka, rainbow trout, tilapia, fugu, zebrafish, black bream, half smooth tongue sole, and grouper (1,35,40), and the expression patterns of these genes are different in male and female gonads (11,33,34,51,65). This gene is considered to affect mainly sex determination and development (55).…”

Section: Discussionmentioning

confidence: 99%

Discovery and identification of candidate sex-related genes based on transcriptome sequencing of Russian sturgeon (Acipenser gueldenstaedtii) gonads

Chen

Xia²,

Shao

et al. 2016

Physiological Genomics

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Discovery and identification of candidate sex-related genes based on transcriptome sequencing of Russian sturgeon (Acipenser gueldenstaedtii) gonads. Physiol Genomics 48: 464 -476, 2016. First published May 6, 2016 doi:10.1152/physiolgenomics.00113.2015.-As the Russian sturgeon (Acipenser gueldenstaedtii) is an important food and is the main source of caviar, it is necessary to discover the genes associated with its sex differentiation. However, the complicated life and maturity cycles of the Russian sturgeon restrict the accurate identification of sex in early development. To generate a first look at specific sex-related genes, we sequenced the transcriptome of gonads in different development stages (1, 2, and 5 yr old stages) with next-generation RNA sequencing. We generated Ͼ60 million raw reads, and the filtered reads were assembled into 263,341 contigs, which produced 38,505 unigenes. Genes involved in signal transduction mechanisms were the most abundant, suggesting that development of sturgeon gonads is under control of signal transduction mechanisms. Differentially expressed gene analysis suggests that more genes for protein synthesis, cytochrome c oxidase subunits, and ribosomal proteins were expressed in female gonads than in male. Meanwhile, male gonads expressed more transposable element transposase, reverse transcriptase, and transposase-related genes than female. In total, 342, 782, and 7,845 genes were detected in intersex, male, and female transcriptomes, respectively. The female gonad expressed more genes than the male gonad, and more genes were involved in female gonadal development. Genes (sox9, foxl2) are differentially expressed in different sexes and may be important sex-related genes in Russian sturgeon. Sox9 genes are responsible for the development of male gonads and foxl2 for female gonads.

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“…However, by performing genomewide linkage analyses, Kikuchi et al [2007] revealed that the sex of fugu is determined by a single region on linkage group 19 in an XX/XY system. As a part of understanding the mechanism of gonadal sex determination, differentiation and development of fugu, histological investigation of the process of gonadal sex differentiation has been carried out previously in our laboratory and the course of ovarian and testicular development in this species was identified [Matsuura et al, 1994;Yamaguchi et al, 2006]. In a recent study with fugu Dmrt ( Doublesex/ mab3 related transcription factor 1), a transcription factor that regulates early differentiation of Sertoli cells in the testes of vertebrates, we have found that it is involved in gonadal development rather than sex differentiation and that its expression is correlated with the proliferation of spermatogonia [Yamaguchi et al, 2006].…”

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confidence: 99%

Fugu <i>(Takifugu rubripes)</i> Sexual Differentiation: CYP19 Regulation and Aromatase Inhibitor Induced Testicular Development

Rashid¹,

Kitano²,

Lee³

et al. 2007

Sex Dev

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In order to assess the involvement of aromatase CYP19 isoforms and endogenous sex steroids in gonadal sex differentiation and development of the Japanese fugu (Takifugu rubripes), an aromatase inhibitor (AI, fadrozole) was administered to developing fishes from the ‘first feeding’ till the 100th day after hatching. It was observed that ovarian cavity formation was inhibited by fadrozole at doses of 500 and 1000 µg/g diet, which was followed by testicular differentiation in all treated fugu. In the non-treated fugu, CYP19A was predominantly expressed in the ovary and CYP19B in the brain (in both sexes), although both were expressed interchangeably at low levels. An exceptionally high expression of CYP19B was also evident in testis throughout the study period. Both forms of CYP19 mRNA showed low levels of expression in brain and gonad with no significant differences between the two AI treatments. AI treatment inhibited CYP19A mRNA in trunk during the crucial period of ovarian cavity formation and CYP19B in gonad and brain by the end of gonadal sex differentiation. An elevation of testosterone and 11-ketotestosterone was observed which can be associated with the down-regulation of the circulating 17β-estradiol production during the AI treatment period. After stopping AI treatment, both circulating estrogen and androgen were normalized. The current results suggest that suppression of CYP19A before and during morphological sex differentiation inhibits ovarian cavity formation in fugu. Furthermore, non-detectable limits of 17β-estradiol and high testosterone levels by the end of the gonadal differentiation period can be ascribed to inhibition of CYP19B, suggesting that conversion of 17β-estradiol from testosterone is plausibly regulated by CYP19B, and that this factor (CYP19B) may play an important role in AI-induced testicular development after gonadal sex differentiation through regulation of the testosterone–17β-estradiol balance in fugu.

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Expression of the DMRT gene and its roles in early gonadal development of the Japanese pufferfish Takifugu rubripes

Cited by 64 publications

References 29 publications

Sexually dimorphic and ontogenetic expression of dmrt1, cyp19a1a and cyp19a1b in Gobiocypris rarus

Sexually dimorphic and ontogenetic expression of dmrt1, cyp19a1a and cyp19a1b in Gobiocypris rarus

Discovery and identification of candidate sex-related genes based on transcriptome sequencing of Russian sturgeon (Acipenser gueldenstaedtii) gonads

Fugu <i>(Takifugu rubripes)</i> Sexual Differentiation: CYP19 Regulation and Aromatase Inhibitor Induced Testicular Development

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