Expression of the drosophila gene <i>disconnected</i> using the UAS/GAL4 system

Robertson, Laura; Dey, Bijan K.; Campos, Ana Regina Nascimento; Mahaffey, James W.

doi:10.1002/gene.10123

Cited by 9 publications

(6 citation statements)

References 17 publications

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“…Ectopic disco expression disrupted other aspects of trunk development. Previously, we showed that DISCO repressed denticle formation (Robertson et al, 2002), and now we find that other aspects of trunk development are also disrupted. The dorsal trachea and oenocytes were absent (Fig.…”

Section: Ectopic Disco Alters Trunk Developmentsupporting

confidence: 60%

“…Considering this, we re-examined the effects of ectopic DISCO to see if this would override the trunk specification system, transforming the trunk to a head identity. In embryos ubiquitously expressing disco, germband contraction fails and a hole appears in the dorsal epidermis, indicating that DISCO may interfere with dorsal closure (Robertson et al, 2002). We stained prd→disco embryos with anti-Engrailed/Invected (EN) antibodies (DiNardo et al, 1985) to monitor the fate of cells in the posterior compartments of the trunk segments, as these cells, and a few cells anterior to these, would be expressing disco.…”

Section: Ectopic Disco Alters Trunk Developmentmentioning

confidence: 99%

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An interactive network of zinc-finger proteins contributes to regionalization of the Drosophila embryo and establishes the domains of HOM-C protein function

et al. 2004

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During animal development, the HOM-C/HOX proteins direct axial patterning by regulating region-specific expression of downstream target genes. Though much is known about these pathways, significant questions remain regarding the mechanisms of specific target gene recognition and regulation, and the role of co-factors. From our studies of the gnathal and trunk-specification proteins Disconnected (DISCO) and Teashirt (TSH), respectively, we present evidence for a network of zinc-finger transcription factors that regionalize the Drosophila embryo. Not only do these proteins establish specific regions within the embryo, but their distribution also establishes where specific HOM-C proteins can function. In this manner, these factors function in parallel to the HOM-C proteins during axial specification. We also show that in tsh mutants, disco is expressed in the trunk segments, probably explaining the partial trunk to head transformation reported in these mutants, but more importantly demonstrating interactions between members of this regionalization network. We conclude that a combination of regionalizing factors, in concert with the HOM-C proteins,promotes the specification of individual segment identity.

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Section: Ectopic Disco Alters Trunk Developmentsupporting

confidence: 60%

Section: Ectopic Disco Alters Trunk Developmentmentioning

confidence: 99%

An interactive network of zinc-finger proteins contributes to regionalization of the Drosophila embryo and establishes the domains of HOM-C protein function

et al. 2004

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“…Most studies -with the exception of those on birds -did not quantify dispersal distance or propensity per se but used proxies related to movement ability during the transfer stage of dispersal, such as flight capacity in insects, and speed or locomotion performance in fish and reptiles (e.g. Garland, 1990;Robertson et al, 2002;Zera & Brisson, 2012;Drangsholt, Damsgard & Olesen, 2014;Mattila & Hanski, 2014; see online Table S1). Morphological traits are also often used as proxies of dispersal.…”

Section: (1) Heritability Of Dispersal and Dispersal-related Traitsmentioning

confidence: 99%

Genetics of dispersal

et al. 2017

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Dispersal is a process of central importance for the ecological and evolutionary dynamics of populations and communities, because of its diverse consequences for gene flow and demography. It is subject to evolutionary change, which begs the question, what is the genetic basis of this potentially complex trait? To address this question, we (i) review the empirical literature on the genetic basis of dispersal, (ii) explore how theoretical investigations of the evolution of dispersal have represented the genetics of dispersal, and (iii) discuss how the genetic basis of dispersal influences theoretical predictions of the evolution of dispersal and potential consequences.Dispersal has a detectable genetic basis in many organisms, from bacteria to plants and animals. Generally, there is evidence for significant genetic variation for dispersal or dispersal‐related phenotypes or evidence for the micro‐evolution of dispersal in natural populations. Dispersal is typically the outcome of several interacting traits, and this complexity is reflected in its genetic architecture: while some genes of moderate to large effect can influence certain aspects of dispersal, dispersal traits are typically polygenic. Correlations among dispersal traits as well as between dispersal traits and other traits under selection are common, and the genetic basis of dispersal can be highly environment‐dependent.By contrast, models have historically considered a highly simplified genetic architecture of dispersal. It is only recently that models have started to consider multiple loci influencing dispersal, as well as non‐additive effects such as dominance and epistasis, showing that the genetic basis of dispersal can influence evolutionary rates and outcomes, especially under non‐equilibrium conditions. For example, the number of loci controlling dispersal can influence projected rates of dispersal evolution during range shifts and corresponding demographic impacts. Incorporating more realism in the genetic architecture of dispersal is thus necessary to enable models to move beyond the purely theoretical towards making more useful predictions of evolutionary and ecological dynamics under current and future environmental conditions. To inform these advances, empirical studies need to answer outstanding questions concerning whether specific genes underlie dispersal variation, the genetic architecture of context‐dependent dispersal phenotypes and behaviours, and correlations among dispersal and other traits.

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“…If so, the inability to reduce free Met- R -SO may be the Achilles Heel of fruit flies. To address this possibility, we generated transgenic flies that express yeast fRMsr 24 , 25 . These flies efficiently reduced free Met- R -SO, and we further employed this model to examine the role of this process in lifespan control and other processes.…”

Section: Introductionmentioning

confidence: 99%

Expression of the methionine sulfoxide reductase lost during evolution extends Drosophila lifespan in a methionine-dependent manner

Lee

Kim

et al. 2018

Sci Rep

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Accumulation of oxidized amino acids, including methionine, has been implicated in aging. The ability to reduce one of the products of methionine oxidation, free methionine-R-sulfoxide (Met-R-SO), is widespread in microorganisms, but during evolution this function, conferred by the enzyme fRMsr, was lost in metazoa. We examined whether restoration of the fRMsr function in an animal can alleviate the consequences of methionine oxidation. Ectopic expression of yeast fRMsr supported the ability of Drosophila to catalyze free Met-R-SO reduction without affecting fecundity, food consumption, and response to starvation. fRMsr expression also increased resistance to oxidative stress. Moreover, it extended lifespan of flies in a methionine-dependent manner. Thus, expression of an oxidoreductase lost during evolution can enhance metabolic and redox functions and lead to an increase in lifespan in an animal model. More broadly, our study exposes the potential of a combination of genetic and nutritional strategies in lifespan control.

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Expression of the drosophila gene disconnected using the UAS/GAL4 system

Cited by 9 publications

References 17 publications

An interactive network of zinc-finger proteins contributes to regionalization of the Drosophila embryo and establishes the domains of HOM-C protein function

An interactive network of zinc-finger proteins contributes to regionalization of the Drosophila embryo and establishes the domains of HOM-C protein function

Genetics of dispersal

Expression of the methionine sulfoxide reductase lost during evolution extends Drosophila lifespan in a methionine-dependent manner

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